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High-Efficiency Prime Editing with Optimized, Paired PegRNAs in Plants
Qiupeng Lin, Shuai Jin, Yuan Zong, Hong Yu, Zixu Zhu, Guanwen Liu, Liquan Kou, Yanpeng Wang, Jin-Long Qiu, Jiayang Li & Caixia Gao
Nature Biotechnology
Abstract
Prime editing (PE) applications are limited by low editing efficiency. Here we show that designing prime binding sites with a melting temperature of 30?°C leads to optimal performance in rice and that using two prime editing guide (peg) RNAs in trans encoding the same edits substantially enhances PE efficiency. Together, these approaches boost PE efficiency from 2.9-fold to 17.4-fold. Optimal pegRNAs or pegRNA pairs can be designed with our web application, PlantPegDesigner.
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DOI:10.1038/s41587-021-00868-w |
Title |
High-Efficiency Prime Editing with Optimized, Paired PegRNAs in Plants |
Authors |
Qiupeng Lin, Shuai Jin, Yuan Zong, Hong Yu, Zixu Zhu, Guanwen Liu, Liquan Kou, Yanpeng Wang, Jin-Long Qiu, Jiayang Li & Caixia Gao |
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2021-03-26 |
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Abstract |
Prime editing (PE) applications are limited by low editing efficiency. Here we show that designing prime binding sites with a melting temperature of 30?°C leads to optimal performance in rice and that using two prime editing guide (peg) RNAs in trans encoding the same edits substantially enhances PE efficiency. Together, these approaches boost PE efficiency from 2.9-fold to 17.4-fold. Optimal pegRNAs or pegRNA pairs can be designed with our web application, PlantPegDesigner. |
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Prime editing (PE) applications are limited by low editing efficiency. Here we show that designing prime binding sites with a melting temperature of 30?°C leads to optimal performance in rice and that using two prime editing guide (peg) RNAs in trans encoding the same edits substantially enhances PE efficiency. Together, these approaches boost PE efficiency from 2.9-fold to 17.4-fold. Optimal pegRNAs or pegRNA pairs can be designed with our web application, PlantPegDesigner. |
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