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  Location: Home >> Faculty >> Molecular Agrobiology
  Molecular Agrobiology


Wenxue Zhai


Dr. Wenxue Zhai, Principle Investigator, PhD (1999, Institute of Genetics, CAS). The laboratory mainly focuses on molecular cloning and molecular breeding of rice functional genes, particularly those with important agronomic traits. The present work includes positional cloning of rice bacterial blight resistance genes, breeding bacterial blight resistant hybrid rice with the cloned Xa21 gene, rice and maize molecular breeding.



KEY PUBLICATIONS
1.Jiang G, Liu D, Yin D, Zhou Z, Shi Y, Li C, Zhu L, Zhai W. A rice NBS-ARC gene conferring quantitative resistance to bacterial blight is regulated by a pathogen effector-inducible miRNA. Mol. Plant., 2020, 13, 1752–1767..
 
2.Jiang G, Yin D, Shi Y, Zhou Z, Li C, Liu P, Jia Y, Wang Y, Liu Z, Yu M, Wu X, Zhai W, Zhu L. OsNPR3.3-dependent salicylic acid signaling is involved in recessive gene xa5-mediated immunity to rice bacterial blight. Scientific Reports, 2020, 10: 6313.
 
3.Han J, Xia Z, Liu, P, Li, C, Wang, Y, Guo L, Jiang, G, and Zhai, W. TALEN-based editing of TFIIAy5 changes rice response to Xanthomonas oryzae pv. Oryzae. Scientific Reports, 2020, 10, 2036.
 
4.Jia Y, Li C, Li Q, Liu P, Liu D, Liu Z, Wang Y, Jiang G, Zhai W. Characteristic dissection of Xanthomonas oryzae pv. oryzae responsive microRNAs in rice. Int. J. Mol. Sci., 2020, 21, 785.
 
5.Gao L, Fang Z, Zhou J, Li L, Lu L, Li L, Li T, Chen L, Zhang W, Zhai W, Peng H. Transcriptional insights into the pyramided resistance to rice bacterial blight. Scientific Reports, 2018, 8, 12358.
 
6.Li T, Zhang Z, Peng H, Fang Z, Zhou J, Liu P, Wang Y, Zhu W, Li L, Zhang Q, Chen L, Li L, Liu Z, Zhang W, Zhai W, Lu L, Gao L, 2019. Application of high-throughput amplicon sequencing-based SSR genotyping in genetic background screening. BMC Genomics, 20:444.
 
7.Li H, Ji G, Wang Y, Qian Q, Xu J, Sodmergen, Liu G, Zhao X, Chen M, Zhai W, Li D and Zhu L (2018) WHITE PANICLE3, a Novel Nucleus-Encoded Mitochondrial Protein, Is Essential for  Proper Development and Maintenance of Chloroplasts and Mitochondria in Rice. Front. Plant Sci., 2018, 9:762.
 
8.Zhao J, Liu P, Li C, Chen H, Han J, Guo L, Jiang G, Zhai W. LMM5.1 and LMM5.4, two eukaryotic translation elongation factor 1A-like gene family members, negatively affect cell death and disease resistance in rice. Journal of Genetics and Genomics, 2017, 44: 107-118.
 
9.Jiang G, Yin D, Zhao J, Chen H, Guo L, Zhu L, Zhai W. The rice thylakoid membrane-bound ascorbate peroxidase OsAPX8 functions in tolerance to bacterial blight. Scientific Reports, 2016, 6, 26104.
 
10.  Chen H, Li C, Liu L, Zhao J, Cheng X, Jiang G, Zhai W. The Fd-GOGAT1 mutant gene lc7 confers resistance to Xanthomonas oryzae pv. Oryzae in rice. Scientific Reports, 2016, 6, 26411; doi: 10.1038/srep26411.
 
11.  Jiang G, Xiang Y, Zhao J, Yin D, Zhao X, Zhu L, and Zhai W. Regulation of Inflorescence Branch Development in Rice Through a Novel Pathway Involving the Pentatricopeptide Repeat Protein sped1-D. Genetics, 2014, 197:1395-1407.
 
 
13.Wu L, Liu D, Wu J, Zhang R, Qin Z, Liu D, Li A, Fu D, Zhai W and Mao L Regulation of FLOWERING LOCUS T by a MicroRNA in Brachypodium distachyon. Plant Cell 2013, 25: 4363-4377.
 
14.Peng H, Jiang G, Zhang J, Zhang W, Zhai W. DNA methylation polymorphism and stability in Chinese indica hybrid rice. Sci China Life Sci, 2013, 56 (12): 1097-1106.
 
15.Geng S, Li A, Tang L, Yin L, Wu L, Lei L, Guo X, Zhang X, Jiang G, Zhai W, Wei Y, Zheng Y, Lan X, and Mao L. TaCPK2-A, a calcium-dependent protein kinase gene that is required for wheat powdery mildew resistance enhances bacterial blight resistance in transgenic rice. J. Exp. Bot., 2013, 64 (11): 3125-3136.
 
16.Chen X, Hao L, Pan J, Zheng X, Jiang G, Jin Y, Gu Z, Qian Q, Zhai W, Ma B. SPL5, a cell death and defense-related gene, encodes a putative splicing factor 3b subunit 3(SF3b3) in rice. Molecular Breeding, 2012, 30: 939-949.
 
17.Gao L, Xia Z, Jiang G, Peng H, Zhao X, Zhai W. Generation of marker-free, bacterial blight-resistant transgenic sterile line and hybrid rice with Xa21. Plant Breeding, 2011, 130: 438-443.
 
18.Zhao F, Cai Z, Hu T, Yao H, Wang L, Dong N, Wang B, Ru Z, Zhai W. Genetic analysis and molecular mapping of a novel gene conferring resistance to rice stripe virus. Plant Mol Biol Rep, 2010, 28: 512–518.
 
19.Chen X, Pan J, Cheng J, Jiang G, Jin Y, Gu Z, Qian Q, Zhai W, Ma B. Fine genetic mapping and physical delimitation of the lesion mimic gene spotted leaf 5 (spl5) in rice (Oryza sativa). Molecular Breeding, 2009, 24: 387-395.
 
20.Jiang L, Zhang W, Xia Z, Jiang G, Qian Q, Li A, Cheng Z, Zhu L, Mao L, Zhai W. A paracentric inversion suppresses genetic recombination at the FON3 locus with breakpoints corresponding to sequence gaps on rice chromosome 11L. Mol Gen Genomics, 2007, 277: 263-272.
 
21.Yu B, Lin Z, Li H, Li X, Li J, Wang Y, Zhang X, Zhu Z, Zhai W, Wang X, Xie D, Sun C. TAC1, a major quantitative trait locus controlling tiller angle in rice. The Plant Journal, 2007, 52: 891-898.
 
22.Zou J, Chen Z, Zhang S, Zhang W, Li G, Chen Z, Zhai W, Zhao X, Pan X, Xie Q, Zhu L. The rice HIGH-TILLERING DWARF1 encoding an ortholog of Arabidopsis MAX3 is required for negative regulation of the outgrowth of axillary buds. Plant J., 2006, 48: 687-698.
 
23.Jiang G, Xia Z, Zhou Y, Wan J,Li D, Chen R, Zhai W, Zhu L. Testifying the rice bacterial blight resistance gene xa5 in its function and further analyzing xa5 (Xa5) by comparing with it’s homolog TFIIAγ1. Mol Gen Genomics. 2006, 275: 354-366.
 
24.Chen X, Shang J, Chen D, Lei C, Zou Y, Zhai W,Liu G, Xu J, Ling Z, Cao G, Ma B, Wang Y, Zhao X, Li S, Zhu L. A B-lectin receptor kinase gene conferring rice blast resistance. Plant J., 2006, 46: 794-804.
 
25.Chen C, Xiao H, Zhang W, Wang A, Xia Z, Li X, Zhai W, Cheng Z, Zhu L. Adapting rice anther culture to gene transformation and RNA interference. Science in China Series C: Life Sciences, 2006, 49(5):414-428.
 
26.Zou J, Chen Z, Zhang S, Zhang W, Jiang G, Zhao X, Zhai W, Pan X, Zhu L. Characterizations and fine mapping of a mutant gene for high tillering and dwarf in rice ( Oryza sativaL.). Planta.2005, 222: 604-612.
 
27.Luo Q, Zhou K, Zhao X, Zeng Q, Xia H, Zhai W, Xu J, Wu X, Yang H, Zhu L. Identification and fine mapping of a mutant gene for palealess spikelet in rice. Planta, 2005, 221: 222-230.
 
28.Guddeti G, Zhang D, Leseberg C, Kang H, Zhai W, Johns M, Mao L. Molecular evolution of the rice miR395 gene family. Cell Research, 2005, 15(8): 631-638.
 
29.Jiang L, Qian Q, Mao L, Zhou Q, Zhai W. Characterization of the rice floral organ number mutant fon3. Journal of Integrative Plant Biology, 2005, 47(1): 100-106.