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  Location: Home >> Faculty >> Molecular Agrobiology
  Molecular Agrobiology

Caixia Gao


1994-1997: Ph.D. China Agricultural University

1991-1994: M.S. Gansu Agricultural University 

1987-1991: B.S. Gansu Agricultural University   



2009-present: Principal investigator, Molecular AgroBiology Center, Institute of Genetics & Developmental Biology, CAS, China 

1998-2009: Research Scientist, Research Division, DLF-Trifolium Group, Denmark 

1997-1998: Postdoc, Research Division, DLF-Trifolium Group, Denmark 


Research Interests: 

Developing novel technologies to achieve efficient and specific genome engineering, and applying them to study the function of genes and modify plant traits for high-quality, disease resistance and stress tolerance in crop species.  

PUBLICATIONS(* Corresponding author):
1. Zhang R1, Liu J1, Chai Z, Chen S, Bai Y, Zong Y, Chen K, Li J, Jiang L, and Gao C* (2019) Generation of herbicide tolerance traits and a new selectable marker in wheat using base editing. Nature Plants, doi: 10.1038/s41477-019-0405-0. (1 co-first authors).
2. Jin S1, Zong Y1, Gao Q1, Zhu Z, Wang Y, Qin P, Liang C, Wang D, Qiu J, Zhang F, Gao C* (2019) Cytosine, but not adenine, base editors induce genome-wide off-target mutations in rice. Science, 364, 292-295. (1 co-first authors).
3. Chen K1, Wang Y1, Zhang R, Zhang H, Gao C* (2019) CRISPR/Cas Genome editing and precision plant breeding in agriculture. Annual Review of Plant Biology, doi: 10.1146/annurev-arplant-050718-100049. (1 co-first authors).
4. Ji X1, Si X1, Zhang Y, Zhang H, Zhang F, Gao C* (2018) Conferring DNA virus resistance with high specificity in plants using virus-inducible genome editing system. Genome Biology, 19, 197.
5. Zhang Y, Massel K, Godwin I, Gao C* (2018) Applications and potential of genome editing in crop improvement. Genome Biology, 19, 210.
6. Li T1, Yang X1, Yu Y1, Si X, Zhai X, Zhang H, Dong W, Gao C*, Xu C* (2018) Domestication of wild tomato is accelerated by genome editing. Nature Biotechnology, 36, 1160-1163. (1 co-first authors).
7. Zong Y1, Song Q1, Li C, Jin S, Zhang D, Wang Y, Qiu JL, Gao C* (2018) Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A. Nature Biotechnology, 36, 950-953. (1 co-first authors).
8. Xue C1, Zhang H1, Lin Q, Fan R, Gao C* (2018) Manipulating mRNA splicing by base editing in plants. Science China Life Sciences. doi: 10.1007/s11427-018-9392-7. (1 co-first authors).
9. Zhang H1, Si X1, Ji X1, Fan R, Liu J, Chen K, Wang Dao, Gao C* (2018) Genome editing of upstream open reading frames (uORFs) enables translational control in plants. Nature Biotechnology, 36, 894-898. (1 co-first authors).
10. Guo L1, Cao X1, Liu Y1, Li J, Li Y, Li D, Zhang K, Gao C, Dong A, Liu X* (2018) A chromatin loop represses WUSCHEL expression in Arabidopsis. Plant Journal, 94, 1083-1097. (1 co-first authors).
11. Li C1, Zong Y1, Wang Y1, Jin S, Zhang D, Song Q, Zhang R, Gao C* (2018) Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biology, 19, 59. (1 co-first authors).
12. Liang Z, Chen K, Zhang Y, Yan Y, and Gao C* (2018) Genotyping genome-edited mutations in plants using CRISPR ribonucleoprotein complexes. Plant Biotechnology Journal, 16, 2053-2062.
13. Bhowmik P*, Ellison E, Polley B, Bollina V, Kulkarni M, Ghanbarnia K, Song H, Gao C, Voytas DF, Kagale S* (2018) Targeted mutagenesis in wheat microspores using CRISPR/Cas9. Scientific Reports, 8, 6502. (1 co-first authors).
14. Zhang Y1, Li D1, Zhang D, Zhao X, Cao X, Dong L, Liu J, Chen K, Zhang H, Gao C*, Wang D* (2018) Analysis of the functions of TaGW2 homoeologs in wheat grain weight and protein content traits. Plant Journal, 94, 857-866. (1 co-first authors).
15. Liang Z1, Chen K1, Zhang Y, Liu J, Yin K, Qiu J, Gao C* (2018) Genome editing of bread wheat using biolistic delivery of CRISPR/Cas9 in vitro transcripts or ribonucleoproteins. Nature Protocols, 13, 413-430. (1 co-first authors).
16. Gao C* (2018) The future of CRISPR technologies in agriculture. Nature Reviews Molecular Cell Biology, 19, 1-2.
17. Zhang D, Xu Z, Cao S, Chen K, Li S, Liu X, Gao C, Zhang B*, Zhou Y* (2018) An uncanonical CCCH-tandem zinc finger protein represses secondary wall synthesis and controls mechanical strength in rice, Molecular Plant, 11, 163-174.
18. Wang M1, Wang S1, Liang Z, Shi W, Gao C*, Xia G* (2018) From Genetic Stock to Genome Editing: Gene Exploitation in Wheat, Trends in Biotechnology, 36, 160-172. (1 co-first authors).
19. Zhang Y, Gao C* (2017) Recent advances in DNA-free editing and precise base editing in plants. Emerging Topics in Life Sciences, 1, 161-168.
20. Wang Y1, Yu H1, Tian C, Sajjad M, Gao C, Tong Y, Wang X*, Jiao Y* (2017) Transcriptome association identifies regulators of wheat spike architecture. Plant Physiology, 175, 746-757.
21. Tu M, Lin L, Cheng Y, He X, Sun H, Xie H, Fu J, Liu C, Li J, Chen D, Xi H, Xue D, Liu Q, Zhao J, Gao C, Song Z, Qu J and Gu F* (2017) A ’new lease of life’: FnCpf1 possesses DNA cleavage activity for genome editing in human cells. Nucleic Acids Research, 45, 11295-11304.
22. Zhang D1, Zhang H1, Li T, Chen K, Qiu J, Gao C* (2017) Perfectly Matched 20-nucleotide Guide RNA Sequences Enable Robust Genome Editing Using High-fidelity SpCas9 Nucleases, Genome Biology, 18, 191. (1 co-first authors).
23. Wang C1, Liu W1, Wang G1, Li J, Dong L, Han L, Wang Q, Tian J, Yu Y, Gao C and Kong Z* (2017) KTN80 confers precision to microtubule severing by specific targeting of katanin complexes in plant cells, The EMBO Journal, e201796823. (1 co-first authors).
24. Meng X1, Yu H1, Zhang Y, Zhuang F, Song X, Gao S, Gao C*, Li J* (2017) Construction of a Genome-Wide Mutant Library in Rice Using CRISPR/Cas9. Molecular Plant, 10, 1238-1241. (1 co-first authors).
25. Yin K, Gao C*, Qiu J* (2017) Progress and prospects in plant genome editing, Nature Plants, 3, 17107.
26. Ran Y*, Liang Z, Gao C* (2017) Current and future editing reagent delivery systems for plant genome editing, Science China Life Science, 60, 490-505.
27. Zhang Y1, Bai Y1, Wu G, Zou S, Chen Y, Gao C*, Tang D* (2017) Simultaneous modification of three homoeologs of TaEDR1 by genome editing enhances powdery mildew resistance in wheat, Plant Journal, 91, 714. (1 co-first authors).
28. Li J1, Zhang H1, Si X, Tian Y, Chen K, Liu J, Chen H, Gao C* (2017) Generation of thermosensitive male-sterile maize by targeted knockout of the ZmTMS5 gene. Journal of Genetics and Genomics, 44, 465-468. (1 co-first authors).
29. Zong Y1, Wang Y1, Li C, Zhang R, Chen K, Ran Y, Qiu J, Wang D, Gao C* (2017) Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion, Nature Biotechnology, 35, 438. (1 co-first authors).
30. Liang Z1, Chen K1, Li T, Zhang Y, Wang Y, Zhao Q, Liu J, Zhang H, Liu C, Ran Y, Gao C* (2017) Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes, Nature Communications, 8, 14261. (1 co-first authors) (Recommended in F1000Prime).
31. Gil-Humanes J, Wang Y, Liang Z, Shan Q, Ozuna C, Sanchez-Leon S, Baltes N, Starker C, Francisco Barro F, Gao C and Voytas DF* (2017) High-efficiency gene targeting in hexaploid wheat using DNA replicons and CRISPR/Cas9. Plant Journal, 89, 1251-1262.
32. Li J1, Meng X1, Zong Y, Chen K, Zhang H, Liu J, Li J*, Gao C* (2016) Gene replacements and insertions in rice by intron targeting using CRISPR/Cas9. Nature Plants, 2 (10), 16139. (1 co-first authors) (Recommended in F1000Prime).
33. Zhang Y1, Liang Z1, Zong Y1, Wang Y, Liu J, Chen K, Qiu J, Gao C* (2016) Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA. Nature Communications, 7, 12617. (1 co-first authors).
34. Jiao, R.*, Gao C* (2016) The CRISPR/Cas9 genome editing revolution. J. Genet. Genomics, 43, 227-228. [Editorial review]
35. Li, X. H., Xia, K., Liang, Z., Chen, K., Gao, C., & Zhang, M* (2016) MicroRNA393 is involved in nitrogen-promoted rice tillering through regulation of auxin signal transduction in axillary buds. Scientific Reports, 6, 32158.
36. Ji X1, Zhang H1, Zhang Y, Wang Y, Gao C* (2015) Establishing a CRISPR-Cas-like immue system conferring DNA virus resistance in plants. Nature Plants, 1, 15144. (1 co-first authors).
37. Wang S, Li S, Liu Q, Wu K, Zhang J, Wang S, Wang Y, Chen X, Zhang Y, Gao C, Wang F, Huang H, Fu X* (2015) The OsSPL16-GW7 regulatory module determines grain shape and simultaneously improves rice yield and grain quality. Nature Genetics, 47, 949.
38. Shan Q, Zhang Y, Chen K, Zhang K & Gao C* (2015) Creation of fragrant rice by targeted knockout of the OsBADH2 gene using TALEN technology. Plant Biotechnology Journal, 13, 791-800.
39. Zhang K, Liu J, Zhang Y, Yang Z & Gao C* (2015) Biolistic genetic transformation of a wide range of Chinese elite wheat (Triticum aestivum L.) varieties. Journal of Genetics and Genomics, 42, 39-42.
40. Shan Q, Wang Y, Li J, Gao C*(2014) Genome editing in rice and wheat using the CRISPR/Cas system. Nature Protocols, 9, 2395-2410.
41. Gao C* (2015) Genome editing in crops: from bench to field. National Science Review, 2, 13-15.
42. Wang Y1, Cheng X1, Shan Q, Zhang Y, Liu J, Gao C*, Qiu J * (2014) Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew. Nature Biotechnology, 32, 947-951. (1 co-first authors) (Recommended in F1000Prime).
43. Xiao J, Xu S, Li C, Xu Y, Xing L, Niu Y, Huan Q, Tang Y, Zhao C, Wagner D, Gao C, Chong K* (2014) O-GlcNAc-mediated interaction between VER2 and TaGRP2 elicits TaVRN1 mRNA accumulation during vernalization in winter wheat. Nature Communications, 5, 4572.
44. Voytas D F*, Gao, C* (2014) Precision genome engineering and agriculture: opportunities and regulatory challenges. PLoS Biology, 12, e1001877.
45. Liang Z, Zhang K, Chen K, Gao C* (2014) Targeted Mutagenesis in Zea mays Using TALENs and the CRISPR/Cas System. Journal of Genetics and Genomics, 41, 63-68.
46. Chen K, Shan Q, Gao C* (2014) An efficient TALEN mutagenesis system in rice. Methods, 69, 2-8.
47. Chen K, Gao C* (2014) Targeted genome modification technologies and their applications in crop improvements. Plant Cell Reports, 33, 575-583.
48. Shan Q1, Wang Y1, Li J1, Zhang Y, Chen K, Liang Z, Zhang K, Liu J, Qiu J*, Gao C* (2013) Targeted genome modification of crop plants using a CRISPR-Cas system. Nature Biotechnology, 31, 686-688. (1 co-first authors).
49. Chen K, Gao C* (2013) TALENs: Customizable Molecular DNA Scissors for Genome Engineering of Plants. Journal of Genetics and Genomics, 40, 271-279.
50. Shan Q1, Wang Y1, Chen K1, Liang Z, Li J, Zhang Y, Zhang K, Liu J, Voytas D F, Zheng X, Zhang Y*, Gao C* (2013) Rapid and efficient gene modification in rice and Brachypodium using TALENs. Molecular Plant, 6, 1365-1368. (1 co-first authors).
Book Chapters
1. Yanpeng Wang, Yuan Zong, Caixia Gao* (2017) Targeted Mutagenesis in Hexaploid Bread Wheat Using the TALEN and CRISPR/Cas Systems. In: Bhalla P., Singh M. (eds) Wheat Biotechnology. Methods in Molecular Biology, vol 1679, 169-185
2. Zhen Liang, Yuan Zong, Caixia Gao* (2016) An Efficient Targeted Mutagenesis System Using CRISPR/Cas in Monocotyledons. Current Protocols in Plant Biology 1, 329-344.
3. Yi Zhang, Jun Li, Caixia Gao* (2016) Generation of Stable Transgenic Rice (Oryza sativa L.) by Agrobacterium-Mediated Transformation. Current Protocols in Plant Biology 1, 235-246.
4. Chen K, Gao C* (2015) Develping CRISPR technology in major crop plants. F. Zhang et al. (eds.), Advances in New Technology for Targeted Modification of Planty Genomes. P145-161. DOI 10.1007/978-1-4939-2556-8_8.
5. Chen K, Gao C* (2015) Targeted gene mutation in plants. X.-Q. Li et al. (eds.), Somatic Genome Manipulation. p253-272. DOI 10.1007/978-1-4939-2389-2_12.
6. Li J., Meng X., Li J., Gao C* (2019) Gene Replacement by Intron Targeting with CRISPR-Cas9. In: Qi Y. (eds) Plant Genome Editing with CRISPR Systems. Methods in Molecular Biology, vol 1917. 285-296
7. Liang Z., Chen K., Gao C* (2019) Biolistic Delivery of CRISPR/Cas9 with Ribonucleoprotein Complex in Wheat. In: Qi Y. (eds) Plant Genome Editing with CRISPR Systems. Methods in Molecular Biology, vol 1917, 311-326