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The effect of collagen-binding NGF-β on the promotion of sciatic nerve regeneration in a rat sciatic nerve crush injury model
Wenjie Sun,Changkai Sun,Hang Lin,Hui Zhao,Jingyu Wang,Hui Ma,Bing Chen,Zhifeng Xiao and Jianwu Dai
Biomaterials
Abstract
Nerve growth factor plays a critical role in peripheral nerve regeneration. However, the lack of efficient NGF delivery approach limits its clinical application. It has demonstrated in our previous work that the native human NGF-β (NAT-NGF) fused with a collagen-binding domain (CBD) could bind to collagen specifically. Since collagen is the major component of nerve extracellular matrix, we speculated that the collagen-binding NGF would target to nerve cells and improve their regeneration. In this report, we found that the fusion protein could specifically bind to endogenous collagen of the rat sciatic nerves and maintain NGF activity both in vitro and in vivo. In the rat sciatic nerve crush injury model, we found that collagen-binding NGF could be retained and concentrated at the nerve injured site to promote nerve repair and enhance function recovery following nerve damage. Thus, the collagen-binding NGF could improve the repair of peripheral nerve injury.
Keywords: Collagen; Nerve growth factor; Nerve; Recombinant protein; Wound healing
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| 论文编号: |
DOI:10.1016/j.biomaterials.2009.05.037 |
| 论文题目: |
The effect of collagen-binding NGF-β on the promotion of sciatic nerve regeneration in a rat sciatic nerve crush injury model |
| 英文论文题目: |
The effect of collagen-binding NGF-β on the promotion of sciatic nerve regeneration in a rat sciatic nerve crush injury model |
| 第一作者: |
Wenjie Sun,Changkai Sun,Hang Lin,Hui Zhao,Jingyu Wang,Hui Ma,Bing Chen,Zhifeng Xiao and Jianwu Dai |
| 英文第一作者: |
Wenjie Sun,Changkai Sun,Hang Lin,Hui Zhao,Jingyu Wang,Hui Ma,Bing Chen,Zhifeng Xiao and Jianwu Dai |
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2009-7-2 |
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| 摘要: |
Nerve growth factor plays a critical role in peripheral nerve regeneration. However, the lack of efficient NGF delivery approach limits its clinical application. It has demonstrated in our previous work that the native human NGF-β (NAT-NGF) fused with a collagen-binding domain (CBD) could bind to collagen specifically. Since collagen is the major component of nerve extracellular matrix, we speculated that the collagen-binding NGF would target to nerve cells and improve their regeneration. In this report, we found that the fusion protein could specifically bind to endogenous collagen of the rat sciatic nerves and maintain NGF activity both in vitro and in vivo. In the rat sciatic nerve crush injury model, we found that collagen-binding NGF could be retained and concentrated at the nerve injured site to promote nerve repair and enhance function recovery following nerve damage. Thus, the collagen-binding NGF could improve the repair of peripheral nerve injury.
Keywords: Collagen; Nerve growth factor; Nerve; Recombinant protein; Wound healing |
| 英文摘要: |
Nerve growth factor plays a critical role in peripheral nerve regeneration. However, the lack of efficient NGF delivery approach limits its clinical application. It has demonstrated in our previous work that the native human NGF-β (NAT-NGF) fused with a collagen-binding domain (CBD) could bind to collagen specifically. Since collagen is the major component of nerve extracellular matrix, we speculated that the collagen-binding NGF would target to nerve cells and improve their regeneration. In this report, we found that the fusion protein could specifically bind to endogenous collagen of the rat sciatic nerves and maintain NGF activity both in vitro and in vivo. In the rat sciatic nerve crush injury model, we found that collagen-binding NGF could be retained and concentrated at the nerve injured site to promote nerve repair and enhance function recovery following nerve damage. Thus, the collagen-binding NGF could improve the repair of peripheral nerve injury.
Keywords: Collagen; Nerve growth factor; Nerve; Recombinant protein; Wound healing |
| 刊物名称: |
Biomaterials |
| 英文刊物名称: |
Biomaterials |
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Wenjie Sun,Changkai Sun,Hang Lin,Hui Zhao,Jingyu Wang,Hui Ma,Bing Chen,Zhifeng Xiao and Jianwu Dai. The effect of collagen-binding NGF-β on the promotion of sciatic nerve regeneration in a rat sciatic nerve crush injury model. Biomaterials. DOI:10.1016/j.biomaterials.2009.05.037 |
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