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Soybean GmPHD-Type Transcription Regulators Improve Stress Tolerance in Transgenic Arabidopsis Plants
Wei Wei,Jian Huang,Yu-Jun Hao,Hong-Feng Zou,Hui-Wen Wang,Jing-Yun Zhao,Xue-Yi Liu,Wan-Ke Zhang,Biao Ma,Jin-Song Zhang,Shou-Yi Chen
PLOS ONE
Abstract
Background
Soybean [Glycine max (L.) Merr.] is one of the most important crops for oil and protein resource. Improvement of stress tolerance will be beneficial for soybean seed production.
Principal Findings
Six GmPHD genes encoding Alfin1-type PHD finger protein were identified and their expressions differentially responded to drought, salt, cold and ABA treatments. The six GmPHDs were nuclear proteins and showed ability to bind the cis-element “GTGGAG”. The N-terminal domain of GmPHD played a major role in DNA binding. Using a protoplast assay system, we find that GmPHD1 to GmPHD5 had transcriptional suppression activity whereas GmPHD6 did not have. In yeast assay, the GmPHD6 can form homodimer and heterodimer with the other GmPHDs except GmPHD2. The N-terminal plus the variable regions but not the PHD-finger is required for the dimerization. Transgenic Arabidopsis plants overexpressing the GmPHD2 showed salt tolerance when compared with the wild type plants. This tolerance was likely achieved by diminishing the oxidative stress through regulation of downstream genes.
Significance
These results provide important clues for soybean stress tolerance through manipulation of PHD-type transcription regulator.
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| 论文编号: |
DOI:10.1371/journal.pone.0007209 |
| 论文题目: |
Soybean GmPHD-Type Transcription Regulators Improve Stress Tolerance in Transgenic Arabidopsis Plants |
| 英文论文题目: |
Soybean GmPHD-Type Transcription Regulators Improve Stress Tolerance in Transgenic Arabidopsis Plants |
| 第一作者: |
Wei Wei,Jian Huang,Yu-Jun Hao,Hong-Feng Zou,Hui-Wen Wang,Jing-Yun Zhao,Xue-Yi Liu,Wan-Ke Zhang,Biao Ma,Jin-Song Zhang,Shou-Yi Chen |
| 英文第一作者: |
Wei Wei,Jian Huang,Yu-Jun Hao,Hong-Feng Zou,Hui-Wen Wang,Jing-Yun Zhao,Xue-Yi Liu,Wan-Ke Zhang,Biao Ma,Jin-Song Zhang,Shou-Yi Chen |
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2009-10-2 |
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| 摘要: |
Background
Soybean [Glycine max (L.) Merr.] is one of the most important crops for oil and protein resource. Improvement of stress tolerance will be beneficial for soybean seed production.
Principal Findings
Six GmPHD genes encoding Alfin1-type PHD finger protein were identified and their expressions differentially responded to drought, salt, cold and ABA treatments. The six GmPHDs were nuclear proteins and showed ability to bind the cis-element “GTGGAG”. The N-terminal domain of GmPHD played a major role in DNA binding. Using a protoplast assay system, we find that GmPHD1 to GmPHD5 had transcriptional suppression activity whereas GmPHD6 did not have. In yeast assay, the GmPHD6 can form homodimer and heterodimer with the other GmPHDs except GmPHD2. The N-terminal plus the variable regions but not the PHD-finger is required for the dimerization. Transgenic Arabidopsis plants overexpressing the GmPHD2 showed salt tolerance when compared with the wild type plants. This tolerance was likely achieved by diminishing the oxidative stress through regulation of downstream genes.
Significance
These results provide important clues for soybean stress tolerance through manipulation of PHD-type transcription regulator.
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| 英文摘要: |
Background
Soybean [Glycine max (L.) Merr.] is one of the most important crops for oil and protein resource. Improvement of stress tolerance will be beneficial for soybean seed production.
Principal Findings
Six GmPHD genes encoding Alfin1-type PHD finger protein were identified and their expressions differentially responded to drought, salt, cold and ABA treatments. The six GmPHDs were nuclear proteins and showed ability to bind the cis-element “GTGGAG”. The N-terminal domain of GmPHD played a major role in DNA binding. Using a protoplast assay system, we find that GmPHD1 to GmPHD5 had transcriptional suppression activity whereas GmPHD6 did not have. In yeast assay, the GmPHD6 can form homodimer and heterodimer with the other GmPHDs except GmPHD2. The N-terminal plus the variable regions but not the PHD-finger is required for the dimerization. Transgenic Arabidopsis plants overexpressing the GmPHD2 showed salt tolerance when compared with the wild type plants. This tolerance was likely achieved by diminishing the oxidative stress through regulation of downstream genes.
Significance
These results provide important clues for soybean stress tolerance through manipulation of PHD-type transcription regulator.
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| 刊物名称: |
PLOS ONE |
| 英文刊物名称: |
PLOS ONE |
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| 其它备注: |
Wei Wei,Jian Huang,Yu-Jun Hao,Hong-Feng Zou,Hui-Wen Wang,Jing-Yun Zhao,Xue-Yi Liu,Wan-Ke Zhang,Biao Ma,Jin-Song Zhang,Shou-Yi Chen. Soybean GmPHD-Type Transcription Regulators Improve Stress Tolerance in Transgenic Arabidopsis Plants. PLOS ONE.DOI:10.1371/journal.pone.0007209 |
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