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RPN1a, a 26S proteasome subunit, is required for innate immunity in Arabidopsis
Chunpeng Yao,Yingying Wu,Haozhen Nie,Dingzhong Tang
The Plant Journal
Abstract
Accumulating evidence shows that proper degradation of proteins affecting defense responses in a positive or negative manner is critical in plant immunity. However, the role of plant degradation systems such as the 26S proteasome in plant immunity is not well understood. Loss-of-function mutations in EDR2 (ENHANCED DISEASE RESISTANCE 2) lead to increased resistance to the adapted biotrophic powdery mildew pathogen Golovinomyces cichoracearum. To study the molecular interactions between powdery mildew pathogen and Arabidopsis, we performed a screen for suppressors of edr2 and found that mutation in the gene encoding RPN1a, a subunit of the 26S proteasome, suppressed edr2-associated disease resistance phenotypes. In addition, RPN1a is required for edr1 and pmr4-mediated powdery mildew resistance and mildew-induced cell death. Furthermore, we show that rpn1a displayed enhanced susceptibility to the fungal pathogen G. cichoracearum and to virulent and avirulent bacterial Pto DC3000 strains, indicating that rpn1a has defects in basal defense and Resistance (R) protein mediated defense. RPN1a-GFP localizes to both the nucleus and cytoplasm. Accumulation of RPN1a is affected by salicylic acid (SA) and the rpn1a mutant has defects in SA accumulation upon Pto DC3000 infection. Further analysis revealed that two other subunits of the 26S proteasome, RPT2a and RPN8a are also involved in edr2-mediated disease resistance. Based on these results, we conclude that RPN1a is required for basal defense and R protein mediated defense. Our data provide evidence that some subunits of the 26S proteasome are involved in innate immunity in Arabidopsis.
Keywords: RPN1a;powdery mildew;disease resistance;cell death;edr2
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论文编号: |
DOI:10.1111/j.1365-313X.2012.05048.x |
论文题目: |
RPN1a, a 26S proteasome subunit, is required for innate immunity in Arabidopsis |
英文论文题目: |
RPN1a, a 26S proteasome subunit, is required for innate immunity in Arabidopsis |
第一作者: |
Chunpeng Yao,Yingying Wu,Haozhen Nie,Dingzhong Tang |
英文第一作者: |
Chunpeng Yao,Yingying Wu,Haozhen Nie,Dingzhong Tang |
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2012-05-14 |
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摘要: |
Accumulating evidence shows that proper degradation of proteins affecting defense responses in a positive or negative manner is critical in plant immunity. However, the role of plant degradation systems such as the 26S proteasome in plant immunity is not well understood. Loss-of-function mutations in EDR2 (ENHANCED DISEASE RESISTANCE 2) lead to increased resistance to the adapted biotrophic powdery mildew pathogen Golovinomyces cichoracearum. To study the molecular interactions between powdery mildew pathogen and Arabidopsis, we performed a screen for suppressors of edr2 and found that mutation in the gene encoding RPN1a, a subunit of the 26S proteasome, suppressed edr2-associated disease resistance phenotypes. In addition, RPN1a is required for edr1 and pmr4-mediated powdery mildew resistance and mildew-induced cell death. Furthermore, we show that rpn1a displayed enhanced susceptibility to the fungal pathogen G. cichoracearum and to virulent and avirulent bacterial Pto DC3000 strains, indicating that rpn1a has defects in basal defense and Resistance (R) protein mediated defense. RPN1a-GFP localizes to both the nucleus and cytoplasm. Accumulation of RPN1a is affected by salicylic acid (SA) and the rpn1a mutant has defects in SA accumulation upon Pto DC3000 infection. Further analysis revealed that two other subunits of the 26S proteasome, RPT2a and RPN8a are also involved in edr2-mediated disease resistance. Based on these results, we conclude that RPN1a is required for basal defense and R protein mediated defense. Our data provide evidence that some subunits of the 26S proteasome are involved in innate immunity in Arabidopsis.
Keywords: RPN1a;powdery mildew;disease resistance;cell death;edr2
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英文摘要: |
Accumulating evidence shows that proper degradation of proteins affecting defense responses in a positive or negative manner is critical in plant immunity. However, the role of plant degradation systems such as the 26S proteasome in plant immunity is not well understood. Loss-of-function mutations in EDR2 (ENHANCED DISEASE RESISTANCE 2) lead to increased resistance to the adapted biotrophic powdery mildew pathogen Golovinomyces cichoracearum. To study the molecular interactions between powdery mildew pathogen and Arabidopsis, we performed a screen for suppressors of edr2 and found that mutation in the gene encoding RPN1a, a subunit of the 26S proteasome, suppressed edr2-associated disease resistance phenotypes. In addition, RPN1a is required for edr1 and pmr4-mediated powdery mildew resistance and mildew-induced cell death. Furthermore, we show that rpn1a displayed enhanced susceptibility to the fungal pathogen G. cichoracearum and to virulent and avirulent bacterial Pto DC3000 strains, indicating that rpn1a has defects in basal defense and Resistance (R) protein mediated defense. RPN1a-GFP localizes to both the nucleus and cytoplasm. Accumulation of RPN1a is affected by salicylic acid (SA) and the rpn1a mutant has defects in SA accumulation upon Pto DC3000 infection. Further analysis revealed that two other subunits of the 26S proteasome, RPT2a and RPN8a are also involved in edr2-mediated disease resistance. Based on these results, we conclude that RPN1a is required for basal defense and R protein mediated defense. Our data provide evidence that some subunits of the 26S proteasome are involved in innate immunity in Arabidopsis.
Keywords: RPN1a;powdery mildew;disease resistance;cell death;edr2
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刊物名称: |
The Plant Journal |
英文刊物名称: |
The Plant Journal |
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Chunpeng Yao,Yingying Wu,Haozhen Nie,Dingzhong Tang. RPN1a, a 26S proteasome subunit, is required for innate immunity in Arabidopsis. The Plant Journal. DOI:10.1111/j.1365-313X.2012.05048.x |
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