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Induction of rat facial nerve regeneration by functional collagen scaffolds
Jiani Cao,Zhifeng Xiao,Wei Jin,Bing Chen,Danqing Meng,Wenyong Ding,Sufang Han,Xiaoshan Hou,Tiansheng Zhu,Baoyu Yuan,Jing Wang,Weibang Liang,Jianwu Dai
Biomaterials
Abstract
Nerve conduit provides a promising strategy for nerve regeneration, and the proper microenvironment in the lumen could improve the regeneration. Our previous work had demonstrated that linear ordered collagen scaffold (LOCS) could effectively guide the oriented growth of axons. Laminin is known as an important nerve growth promoting factor and can facilitate the growth cone formation. In addition, ciliary neurotrophic factor (CNTF) and brain-derived neurotrophic factor (BDNF) can effectively improve the nerve regeneration after nerve injuries. However, in practice, diffusion caused by the body fluids is the major obstacle in their applications. To retain CNTF or BDNF on the scaffolds, we produced collagen binding CNTF (CBD-CNTF), collagen binding BDNF (CBD-BDNF) and laminin binding CNTF (LBD-CNTF), laminin binding BDNF (LBD-BDNF) respectively. In this work, we developed laminin modified LOCS fibers (L × LOCS) by chemical cross-linking LOCS fibers with laminin. Collagen binding or laminin binding neurotrophic factors were combined with LOCS or L × LOCS, and then filled them into the collagen nerve conduit. They were found to guide the ordered growth of axons, and improve the nerve functional recovery in the rat facial nerve transection model. The combination of CNTF and BDNF greatly enhanced the facial nerve regeneration and functional recovery.
Keywords:Neurotrophic factors; Targeting repair; Laminin; Collagen; Peripheral nerve injury
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论文编号: |
DOI:10.1016/j.biomaterials.2012.10.031 |
论文题目: |
Induction of rat facial nerve regeneration by functional collagen scaffolds |
英文论文题目: |
Induction of rat facial nerve regeneration by functional collagen scaffolds |
第一作者: |
Jiani Cao,Zhifeng Xiao,Wei Jin,Bing Chen,Danqing Meng,Wenyong Ding,Sufang Han,Xiaoshan Hou,Tiansheng Zhu,Baoyu Yuan,Jing Wang,Weibang Liang,Jianwu Dai |
英文第一作者: |
Jiani Cao,Zhifeng Xiao,Wei Jin,Bing Chen,Danqing Meng,Wenyong Ding,Sufang Han,Xiaoshan Hou,Tiansheng Zhu,Baoyu Yuan,Jing Wang,Weibang Liang,Jianwu Dai |
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2012-11-07 |
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摘要: |
Nerve conduit provides a promising strategy for nerve regeneration, and the proper microenvironment in the lumen could improve the regeneration. Our previous work had demonstrated that linear ordered collagen scaffold (LOCS) could effectively guide the oriented growth of axons. Laminin is known as an important nerve growth promoting factor and can facilitate the growth cone formation. In addition, ciliary neurotrophic factor (CNTF) and brain-derived neurotrophic factor (BDNF) can effectively improve the nerve regeneration after nerve injuries. However, in practice, diffusion caused by the body fluids is the major obstacle in their applications. To retain CNTF or BDNF on the scaffolds, we produced collagen binding CNTF (CBD-CNTF), collagen binding BDNF (CBD-BDNF) and laminin binding CNTF (LBD-CNTF), laminin binding BDNF (LBD-BDNF) respectively. In this work, we developed laminin modified LOCS fibers (L × LOCS) by chemical cross-linking LOCS fibers with laminin. Collagen binding or laminin binding neurotrophic factors were combined with LOCS or L × LOCS, and then filled them into the collagen nerve conduit. They were found to guide the ordered growth of axons, and improve the nerve functional recovery in the rat facial nerve transection model. The combination of CNTF and BDNF greatly enhanced the facial nerve regeneration and functional recovery.
Keywords:Neurotrophic factors; Targeting repair; Laminin; Collagen; Peripheral nerve injury |
英文摘要: |
Nerve conduit provides a promising strategy for nerve regeneration, and the proper microenvironment in the lumen could improve the regeneration. Our previous work had demonstrated that linear ordered collagen scaffold (LOCS) could effectively guide the oriented growth of axons. Laminin is known as an important nerve growth promoting factor and can facilitate the growth cone formation. In addition, ciliary neurotrophic factor (CNTF) and brain-derived neurotrophic factor (BDNF) can effectively improve the nerve regeneration after nerve injuries. However, in practice, diffusion caused by the body fluids is the major obstacle in their applications. To retain CNTF or BDNF on the scaffolds, we produced collagen binding CNTF (CBD-CNTF), collagen binding BDNF (CBD-BDNF) and laminin binding CNTF (LBD-CNTF), laminin binding BDNF (LBD-BDNF) respectively. In this work, we developed laminin modified LOCS fibers (L × LOCS) by chemical cross-linking LOCS fibers with laminin. Collagen binding or laminin binding neurotrophic factors were combined with LOCS or L × LOCS, and then filled them into the collagen nerve conduit. They were found to guide the ordered growth of axons, and improve the nerve functional recovery in the rat facial nerve transection model. The combination of CNTF and BDNF greatly enhanced the facial nerve regeneration and functional recovery.
Keywords:Neurotrophic factors; Targeting repair; Laminin; Collagen; Peripheral nerve injury |
刊物名称: |
Biomaterials |
英文刊物名称: |
Biomaterials |
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其它备注: |
Jiani Cao,Zhifeng Xiao,Wei Jin,Bing Chen,Danqing Meng,Wenyong Ding,Sufang Han,Xiaoshan Hou,Tiansheng Zhu,Baoyu Yuan,Jing Wang,Weibang Liang,Jianwu Dai. Induction of rat facial nerve regeneration by functional collagen scaffolds. Biomaterials. DOI:10.1016/j.biomaterials.2012.10.031 |
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