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Rare allele of OsPPKL1 associated with grain length causes extra-large grain and a significant yield increase in rice
Xiaojun Zhang,Jianfei Wang,Ji Huang,Hongxia Lan,Cailin Wang,Congfei Yin, Yunyu Wu,Haijuan Tang,Qian Qian,Jiayang Li,and Hongsheng Zhang
PNAS
Abstract
Grain size and shape are important components determining rice grain yield, and they are controlled by quantitative trait loci (QTLs). Here, we report the cloning and functional characterization of a major grain length QTL, qGL3, which encodes a putative protein phosphatase with Kelch-like repeat domain (OsPPKL1). We found a rare allele qgl3 that leads to a long grain phenotype by an aspartate-to-glutamate transition in a conserved AVLDT motif of the second Kelch domain in OsPPKL1. The rice genome has other two OsPPKL1 homologs, OsPPKL2 and OsPPKL3. Transgenic studies showed that OsPPKL1 and OsPPKL3 function as negative regulators of grain length, whereas OsPPKL2 as a positive regulator. The Kelch domains are essential for the OsPPKL1 biological function. Field trials showed that the application of the qgl3 allele could significantly increase grain yield in both inbred and hybrid rice varieties, due to its favorable effect on grain length, filling, and weight.
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论文编号: |
DOI:10.1073/pnas.1219776110 |
论文题目: |
Rare allele of OsPPKL1 associated with grain length causes extra-large grain and a significant yield increase in rice |
英文论文题目: |
Rare allele of OsPPKL1 associated with grain length causes extra-large grain and a significant yield increase in rice |
第一作者: |
Xiaojun Zhang,Jianfei Wang,Ji Huang,Hongxia Lan,Cailin Wang,Congfei Yin, Yunyu Wu,Haijuan Tang,Qian Qian,Jiayang Li,and Hongsheng Zhang |
英文第一作者: |
Xiaojun Zhang,Jianfei Wang,Ji Huang,Hongxia Lan,Cailin Wang,Congfei Yin, Yunyu Wu,Haijuan Tang,Qian Qian,Jiayang Li,and Hongsheng Zhang |
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2012-12-16 |
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摘要: |
Grain size and shape are important components determining rice grain yield, and they are controlled by quantitative trait loci (QTLs). Here, we report the cloning and functional characterization of a major grain length QTL, qGL3, which encodes a putative protein phosphatase with Kelch-like repeat domain (OsPPKL1). We found a rare allele qgl3 that leads to a long grain phenotype by an aspartate-to-glutamate transition in a conserved AVLDT motif of the second Kelch domain in OsPPKL1. The rice genome has other two OsPPKL1 homologs, OsPPKL2 and OsPPKL3. Transgenic studies showed that OsPPKL1 and OsPPKL3 function as negative regulators of grain length, whereas OsPPKL2 as a positive regulator. The Kelch domains are essential for the OsPPKL1 biological function. Field trials showed that the application of the qgl3 allele could significantly increase grain yield in both inbred and hybrid rice varieties, due to its favorable effect on grain length, filling, and weight. |
英文摘要: |
Grain size and shape are important components determining rice grain yield, and they are controlled by quantitative trait loci (QTLs). Here, we report the cloning and functional characterization of a major grain length QTL, qGL3, which encodes a putative protein phosphatase with Kelch-like repeat domain (OsPPKL1). We found a rare allele qgl3 that leads to a long grain phenotype by an aspartate-to-glutamate transition in a conserved AVLDT motif of the second Kelch domain in OsPPKL1. The rice genome has other two OsPPKL1 homologs, OsPPKL2 and OsPPKL3. Transgenic studies showed that OsPPKL1 and OsPPKL3 function as negative regulators of grain length, whereas OsPPKL2 as a positive regulator. The Kelch domains are essential for the OsPPKL1 biological function. Field trials showed that the application of the qgl3 allele could significantly increase grain yield in both inbred and hybrid rice varieties, due to its favorable effect on grain length, filling, and weight. |
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PNAS |
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PNAS |
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Xiaojun Zhang,Jianfei Wang,Ji Huang,Hongxia Lan,Cailin Wang,Congfei Yin, Yunyu Wu,Haijuan Tang,Qian Qian,Jiayang Li,and Hongsheng Zhang.Rare allele of OsPPKL1 associated with grain length causes extra-large grain and a significant yield increase in rice.PNAS.DOI:10.1073/pnas.1219776110 |
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