|
The FLS2-Associated Kinase BIK1 Directly Phosphorylates the NADPH Oxidase RbohD to Control Plant Immunity
Lei Li,Meng Li,Liping Yu,Zhaoyang Zhou,Xiangxiu Liang,Zixu Liu,Gaihong Cai,Liyan Gao,Xiaojuan Zhang,Yingchun Wang,She Chen,and Jian-Min Zhou
Cell Host & Microbe
Abstract
The Arabidopsis immune receptor FLS2 senses the bacterial flagellin epitope flg22 to activate transient elevation of cytosolic calcium ions, production of reactive oxygen species (ROS), and other signaling events to coordinate antimicrobial defenses, such as stomatal closure that limits bacterial invasion. However, how FLS2 regulates these signaling events remains largely unknown. Here we show that the receptor-like cytoplasmic kinase BIK1, a component of the FLS2 immune receptor complex, not only positively regulates flg22-triggered calcium influx but also directly phosphorylates the NADPH oxidase RbohD at specific sites in a calcium-independent manner to enhance ROS generation. Furthermore, BIK1 and RbohD form a pathway that controls stomatal movement in response to flg22, thereby restricting bacterial entry into leaf tissues. These findings highlight a direct role of the FLS2 complex in the regulation of RbohD-mediated ROS production and stomatal defense.
|
论文编号: |
DOI:10.1016/j.chom.2014.02.009 |
论文题目: |
The FLS2-Associated Kinase BIK1 Directly Phosphorylates the NADPH Oxidase RbohD to Control Plant Immunity |
英文论文题目: |
The FLS2-Associated Kinase BIK1 Directly Phosphorylates the NADPH Oxidase RbohD to Control Plant Immunity |
第一作者: |
Lei Li,Meng Li,Liping Yu,Zhaoyang Zhou,Xiangxiu Liang,Zixu Liu,Gaihong Cai,Liyan Gao,Xiaojuan Zhang,Yingchun Wang,She Chen,and Jian-Min Zhou |
英文第一作者: |
Lei Li,Meng Li,Liping Yu,Zhaoyang Zhou,Xiangxiu Liang,Zixu Liu,Gaihong Cai,Liyan Gao,Xiaojuan Zhang,Yingchun Wang,She Chen,and Jian-Min Zhou |
联系作者: |
|
英文联系作者: |
|
外单位作者单位: |
|
英文外单位作者单位: |
|
发表年度: |
2014-03-14 |
卷: |
|
期: |
|
页码: |
|
摘要: |
The Arabidopsis immune receptor FLS2 senses the bacterial flagellin epitope flg22 to activate transient elevation of cytosolic calcium ions, production of reactive oxygen species (ROS), and other signaling events to coordinate antimicrobial defenses, such as stomatal closure that limits bacterial invasion. However, how FLS2 regulates these signaling events remains largely unknown. Here we show that the receptor-like cytoplasmic kinase BIK1, a component of the FLS2 immune receptor complex, not only positively regulates flg22-triggered calcium influx but also directly phosphorylates the NADPH oxidase RbohD at specific sites in a calcium-independent manner to enhance ROS generation. Furthermore, BIK1 and RbohD form a pathway that controls stomatal movement in response to flg22, thereby restricting bacterial entry into leaf tissues. These findings highlight a direct role of the FLS2 complex in the regulation of RbohD-mediated ROS production and stomatal defense. |
英文摘要: |
The Arabidopsis immune receptor FLS2 senses the bacterial flagellin epitope flg22 to activate transient elevation of cytosolic calcium ions, production of reactive oxygen species (ROS), and other signaling events to coordinate antimicrobial defenses, such as stomatal closure that limits bacterial invasion. However, how FLS2 regulates these signaling events remains largely unknown. Here we show that the receptor-like cytoplasmic kinase BIK1, a component of the FLS2 immune receptor complex, not only positively regulates flg22-triggered calcium influx but also directly phosphorylates the NADPH oxidase RbohD at specific sites in a calcium-independent manner to enhance ROS generation. Furthermore, BIK1 and RbohD form a pathway that controls stomatal movement in response to flg22, thereby restricting bacterial entry into leaf tissues. These findings highlight a direct role of the FLS2 complex in the regulation of RbohD-mediated ROS production and stomatal defense. |
刊物名称: |
Cell Host & Microbe |
英文刊物名称: |
Cell Host & Microbe |
论文全文: |
|
英文论文全文: |
|
全文链接: |
|
其它备注: |
Lei Li,Meng Li,Liping Yu,Zhaoyang Zhou,Xiangxiu Liang,Zixu Liu,Gaihong Cai,Liyan Gao,Xiaojuan Zhang,Yingchun Wang,She Chen,and Jian-Min Zhou. The FLS2-Associated Kinase BIK1 Directly Phosphorylates the NADPH Oxidase RbohD to Control Plant Immunity. Cell Host & Microbe. DOI:10.1016/j.chom.2014.02.009 |
英文其它备注: |
|
学科: |
|
英文学科: |
|
影响因子: |
|
第一作者所在部门: |
|
英文第一作者所在部门: |
|
论文出处: |
|
英文论文出处: |
|
论文类别: |
|
英文论文类别: |
|
参与作者: |
|
英文参与作者: |
|
|