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Perlecan Antagonizes Collagen IV and ADAMTS9/GON-1 in Restricting the Growth of Presynaptic Boutons
Jianzhen Qin,Jingjing Liang,and Mei Ding
The Journal of Neuroscience
Abstract
In the mature nervous system, a significant fraction of synapses are structurally stable over a long time scale. However, the mechanisms that restrict synaptic growth within a confined region are poorly understood. Here, we identified that in the C. elegans neuromuscular junction, collagens Type IV and XVIII, and the secreted metalloprotease ADAMTS/GON-1 are critical for growth restriction of presynaptic boutons. Without these components, ectopic boutons progressively invade into the nonsynaptic region. Perlecan/UNC-52 promotes the growth of ectopic boutons and functions antagonistically to collagen Type IV and GON-1 but not to collagen XVIII. The growth constraint of presynaptic boutons correlates with the integrity of the extracellular matrix basal lamina or basement membrane (BM), which surrounds chemical synapses. Fragmented BM appears in the region where ectopic boutons emerge. Further removal of UNC-52 improves the BM integrity and the tight association between BM and presynaptic boutons. Together, our results unravel the complex role of the BM in restricting the growth of presynaptic boutons and reveal the antagonistic function of perlecan on Type IV collagen and ADAMTS protein.
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论文编号: |
DOI:10.1523/JNEUROSCI.5128-13.2014 |
论文题目: |
Perlecan Antagonizes Collagen IV and ADAMTS9/GON-1 in Restricting the Growth of Presynaptic Boutons |
英文论文题目: |
Perlecan Antagonizes Collagen IV and ADAMTS9/GON-1 in Restricting the Growth of Presynaptic Boutons |
第一作者: |
Jianzhen Qin,Jingjing Liang,and Mei Ding |
英文第一作者: |
Jianzhen Qin,Jingjing Liang,and Mei Ding |
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2014-08-13 |
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摘要: |
In the mature nervous system, a significant fraction of synapses are structurally stable over a long time scale. However, the mechanisms that restrict synaptic growth within a confined region are poorly understood. Here, we identified that in the C. elegans neuromuscular junction, collagens Type IV and XVIII, and the secreted metalloprotease ADAMTS/GON-1 are critical for growth restriction of presynaptic boutons. Without these components, ectopic boutons progressively invade into the nonsynaptic region. Perlecan/UNC-52 promotes the growth of ectopic boutons and functions antagonistically to collagen Type IV and GON-1 but not to collagen XVIII. The growth constraint of presynaptic boutons correlates with the integrity of the extracellular matrix basal lamina or basement membrane (BM), which surrounds chemical synapses. Fragmented BM appears in the region where ectopic boutons emerge. Further removal of UNC-52 improves the BM integrity and the tight association between BM and presynaptic boutons. Together, our results unravel the complex role of the BM in restricting the growth of presynaptic boutons and reveal the antagonistic function of perlecan on Type IV collagen and ADAMTS protein. |
英文摘要: |
In the mature nervous system, a significant fraction of synapses are structurally stable over a long time scale. However, the mechanisms that restrict synaptic growth within a confined region are poorly understood. Here, we identified that in the C. elegans neuromuscular junction, collagens Type IV and XVIII, and the secreted metalloprotease ADAMTS/GON-1 are critical for growth restriction of presynaptic boutons. Without these components, ectopic boutons progressively invade into the nonsynaptic region. Perlecan/UNC-52 promotes the growth of ectopic boutons and functions antagonistically to collagen Type IV and GON-1 but not to collagen XVIII. The growth constraint of presynaptic boutons correlates with the integrity of the extracellular matrix basal lamina or basement membrane (BM), which surrounds chemical synapses. Fragmented BM appears in the region where ectopic boutons emerge. Further removal of UNC-52 improves the BM integrity and the tight association between BM and presynaptic boutons. Together, our results unravel the complex role of the BM in restricting the growth of presynaptic boutons and reveal the antagonistic function of perlecan on Type IV collagen and ADAMTS protein. |
刊物名称: |
The Journal of Neuroscience |
英文刊物名称: |
The Journal of Neuroscience |
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其它备注: |
Jianzhen Qin,Jingjing Liang,and Mei Ding. Perlecan Antagonizes Collagen IV and ADAMTS9/GON-1 in Restricting the Growth of Presynaptic Boutons. The Journal of Neuroscience. DOI:10.1523/JNEUROSCI.5128-13.2014 |
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