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Biogenesis of lysosome-related organelles complex-1 subunit 1 (BLOS1) interacts with sorting nexin 2 and the ESCRT-I component TSG101 to mediate the sorting of EGFR into endosomal compartments
Aili Zhang,Xin He,Ling Zhang,Lin Yang,Philip Woodman and Wei Li
THE JOURNAL OF BIOLOGICAL CHEMISTRY
Abstract
Biogenesis of lysosome-related organelles complex-1 (BLOC-1) is a component of the molecular machinery required for the biogenesis of specialized organelles and lysosomal targeting of cargoes via the endosomal to lysosomal trafficking pathway. BLOS1, one subunit of BLOC-1, is implicated in lysosomal trafficking of membrane proteins. We found that the degradation and trafficking of EGFR were delayed in BLOS1 knockdown cells, which were rescued through BLOS1 overexpression. A key feature to the delayed EGFR degradation is the accumulation of endolysosomes in BLOS1 knockdown cells or BLOS1 knockout mouse embryonic fibroblasts. BLOS1 interacted with SNX2 (a retromer subunit) and TSG101 (an ESCRT-I subunit) to mediate EGFR lysosomal trafficking. These results suggest that coordination of the endo-lysosomal trafficking proteins is important for proper targeting of EGFR to lysosomes.
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论文编号: |
DOI:10.1074/jbc.M114.576561 |
论文题目: |
Biogenesis of lysosome-related organelles complex-1 subunit 1 (BLOS1) interacts with sorting nexin 2 and the ESCRT-I component TSG101 to mediate the sorting of EGFR into endosomal compartments |
英文论文题目: |
Biogenesis of lysosome-related organelles complex-1 subunit 1 (BLOS1) interacts with sorting nexin 2 and the ESCRT-I component TSG101 to mediate the sorting of EGFR into endosomal compartments |
第一作者: |
Aili Zhang,Xin He,Ling Zhang,Lin Yang,Philip Woodman and Wei Li |
英文第一作者: |
Aili Zhang,Xin He,Ling Zhang,Lin Yang,Philip Woodman and Wei Li |
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2014-09-03 |
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摘要: |
Biogenesis of lysosome-related organelles complex-1 (BLOC-1) is a component of the molecular machinery required for the biogenesis of specialized organelles and lysosomal targeting of cargoes via the endosomal to lysosomal trafficking pathway. BLOS1, one subunit of BLOC-1, is implicated in lysosomal trafficking of membrane proteins. We found that the degradation and trafficking of EGFR were delayed in BLOS1 knockdown cells, which were rescued through BLOS1 overexpression. A key feature to the delayed EGFR degradation is the accumulation of endolysosomes in BLOS1 knockdown cells or BLOS1 knockout mouse embryonic fibroblasts. BLOS1 interacted with SNX2 (a retromer subunit) and TSG101 (an ESCRT-I subunit) to mediate EGFR lysosomal trafficking. These results suggest that coordination of the endo-lysosomal trafficking proteins is important for proper targeting of EGFR to lysosomes. |
英文摘要: |
Biogenesis of lysosome-related organelles complex-1 (BLOC-1) is a component of the molecular machinery required for the biogenesis of specialized organelles and lysosomal targeting of cargoes via the endosomal to lysosomal trafficking pathway. BLOS1, one subunit of BLOC-1, is implicated in lysosomal trafficking of membrane proteins. We found that the degradation and trafficking of EGFR were delayed in BLOS1 knockdown cells, which were rescued through BLOS1 overexpression. A key feature to the delayed EGFR degradation is the accumulation of endolysosomes in BLOS1 knockdown cells or BLOS1 knockout mouse embryonic fibroblasts. BLOS1 interacted with SNX2 (a retromer subunit) and TSG101 (an ESCRT-I subunit) to mediate EGFR lysosomal trafficking. These results suggest that coordination of the endo-lysosomal trafficking proteins is important for proper targeting of EGFR to lysosomes. |
刊物名称: |
THE JOURNAL OF BIOLOGICAL CHEMISTRY |
英文刊物名称: |
THE JOURNAL OF BIOLOGICAL CHEMISTRY |
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其它备注: |
Aili Zhang,Xin He,Ling Zhang,Lin Yang,Philip Woodman and Wei Li. Biogenesis of lysosome-related organelles complex-1 subunit 1 (BLOS1) interacts with sorting nexin 2 and the ESCRT-I component TSG101 to mediate the sorting of EGFR into endosomal compartments. THE JOURNAL OF BIOLOGICAL CHEMISTRY. DOI:10.1074/jbc.M114.576561 |
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