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RNase ZS1 processesUbL40mRNAs and controls thermosensitive genic male sterility in rice
Hai Zhou,Ming Zhou,Yuanzhu Yang,Jing Li,Liya Zhu,Dagang Jiang,Jingfang Dong,Qinjian Liu,Lianfeng Gu,Lingyan Zhou,Mingji Feng,Peng Qin,Xiaochun Hu,Chengli Song,Jinfeng Shi,Xianwei Song,Erdong Ni,Xiaojin Wu,Qiyun Deng,Zhenlan Liu,Mingsheng Chen,Yao-Guang Liu,Xiaofeng Cao & Chuxiong Zhuang
Nature Communications
Abstract
Thermosensitive genic male-sterile (TGMS) lines, which are male-sterile at restrictive (high) temperatures but male-fertile at permissive (low) temperatures, have been widely used in breeding two-line hybrid rice (Oryza sativa L.). Here we find that mutation of thermosensitive genic male sterile 5 (tms5) in rice causes the TGMS trait through a loss of RNase ZS1 function. We show that RNase ZS1 processes the mRNAs of three ubiquitin fusion ribosomal protein L40 (UbL40) genes into multiple fragments in vitro and in vivo. In tms5 mutants, high temperature results in increased levels of UbL40 mRNAs. Overaccumulation of UbL40 mRNAs causes defective pollen production and male sterility. Our results uncover a novel mechanism of RNase ZS1-mediated UbL40 mRNA regulation and shows that loss of this regulation produces TGMS in rice, a finding with potential applications in hybrid crop breeding.
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DOI:10.1038/ncomms5884 |
论文题目: |
RNase ZS1 processesUbL40mRNAs and controls thermosensitive genic male sterility in rice |
英文论文题目: |
RNase ZS1 processesUbL40mRNAs and controls thermosensitive genic male sterility in rice |
第一作者: |
Hai Zhou,Ming Zhou,Yuanzhu Yang,Jing Li,Liya Zhu,Dagang Jiang,Jingfang Dong,Qinjian Liu,Lianfeng Gu,Lingyan Zhou,Mingji Feng,Peng Qin,Xiaochun Hu,Chengli Song,Jinfeng Shi,Xianwei Song,Erdong Ni,Xiaojin Wu,Qiyun Deng,Zhenlan Liu,Mingsheng Chen,Yao-Guang Liu,Xiaofeng Cao & Chuxiong Zhuang |
英文第一作者: |
Hai Zhou,Ming Zhou,Yuanzhu Yang,Jing Li,Liya Zhu,Dagang Jiang,Jingfang Dong,Qinjian Liu,Lianfeng Gu,Lingyan Zhou,Mingji Feng,Peng Qin,Xiaochun Hu,Chengli Song,Jinfeng Shi,Xianwei Song,Erdong Ni,Xiaojin Wu,Qiyun Deng,Zhenlan Liu,Mingsheng Chen,Yao-Guang Liu,Xiaofeng Cao & Chuxiong Zhuang |
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2014-09-13 |
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摘要: |
Thermosensitive genic male-sterile (TGMS) lines, which are male-sterile at restrictive (high) temperatures but male-fertile at permissive (low) temperatures, have been widely used in breeding two-line hybrid rice (Oryza sativa L.). Here we find that mutation of thermosensitive genic male sterile 5 (tms5) in rice causes the TGMS trait through a loss of RNase ZS1 function. We show that RNase ZS1 processes the mRNAs of three ubiquitin fusion ribosomal protein L40 (UbL40) genes into multiple fragments in vitro and in vivo. In tms5 mutants, high temperature results in increased levels of UbL40 mRNAs. Overaccumulation of UbL40 mRNAs causes defective pollen production and male sterility. Our results uncover a novel mechanism of RNase ZS1-mediated UbL40 mRNA regulation and shows that loss of this regulation produces TGMS in rice, a finding with potential applications in hybrid crop breeding. |
英文摘要: |
Thermosensitive genic male-sterile (TGMS) lines, which are male-sterile at restrictive (high) temperatures but male-fertile at permissive (low) temperatures, have been widely used in breeding two-line hybrid rice (Oryza sativa L.). Here we find that mutation of thermosensitive genic male sterile 5 (tms5) in rice causes the TGMS trait through a loss of RNase ZS1 function. We show that RNase ZS1 processes the mRNAs of three ubiquitin fusion ribosomal protein L40 (UbL40) genes into multiple fragments in vitro and in vivo. In tms5 mutants, high temperature results in increased levels of UbL40 mRNAs. Overaccumulation of UbL40 mRNAs causes defective pollen production and male sterility. Our results uncover a novel mechanism of RNase ZS1-mediated UbL40 mRNA regulation and shows that loss of this regulation produces TGMS in rice, a finding with potential applications in hybrid crop breeding. |
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Nature Communications |
英文刊物名称: |
Nature Communications |
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其它备注: |
Hai Zhou,Ming Zhou,Yuanzhu Yang,Jing Li,Liya Zhu,Dagang Jiang,Jingfang Dong,Qinjian Liu,Lianfeng Gu,Lingyan Zhou,Mingji Feng,Peng Qin,Xiaochun Hu,Chengli Song,Jinfeng Shi,Xianwei Song,Erdong Ni,Xiaojin Wu,Qiyun Deng,Zhenlan Liu,Mingsheng Chen,Yao-Guang Liu,Xiaofeng Cao & Chuxiong Zhuang. RNase ZS1 processesUbL40mRNAs and controls thermosensitive genic male sterility in rice. Nature Communications. DOI:10.1038/ncomms5884 |
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