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An organ boundary-enriched gene regulatory network uncovers regulatory hierarchies underlying axillary meristem initiation
Caihuan Tian,Xiaoni Zhang,Jun He,Haopeng Yu,Ying Wang,Bihai Shi,Yingying Han,Guoxun Wang,Xiaoming Feng,Cui Zhang,Jin Wang,Jiyan Qi,Rong Yu, and Yuling Jiao
Mol.Syst.Biol
Abstract
Gene regulatory networks (GRNs) control development via cell type‐specific gene expression and interactions between transcription factors (TFs) and regulatory promoter regions. Plant organ boundaries separate lateral organs from the apical meristem and harbor axillary meristems (AMs). AMs, as stem cell niches, make the shoot a ramifying system. Although AMs have important functions in plant development, our knowledge of organ boundary and AM formation remains rudimentary. Here, we generated a cellular‐resolution genomewide gene expression map for low‐abundance Arabidopsis thaliana organ boundary cells and constructed a genomewide protein–DNA interaction map focusing on genes affecting boundary and AM formation. The resulting GRN uncovers transcriptional signatures, predicts cellular functions, and identifies promoter hub regions that are bound by many TFs. Importantly, further experimental studies determined the regulatory effects of many TFs on their targets, identifying regulators and regulatory relationships in AM initiation. This systems biology approach thus enhances our understanding of a key developmental process.
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论文编号: |
DOI:10.15252/msb.20145470 |
论文题目: |
An organ boundary-enriched gene regulatory network uncovers regulatory hierarchies underlying axillary meristem initiation |
英文论文题目: |
An organ boundary-enriched gene regulatory network uncovers regulatory hierarchies underlying axillary meristem initiation |
第一作者: |
Caihuan Tian,Xiaoni Zhang,Jun He,Haopeng Yu,Ying Wang,Bihai Shi,Yingying Han,Guoxun Wang,Xiaoming Feng,Cui Zhang,Jin Wang,Jiyan Qi,Rong Yu, and Yuling Jiao |
英文第一作者: |
Caihuan Tian,Xiaoni Zhang,Jun He,Haopeng Yu,Ying Wang,Bihai Shi,Yingying Han,Guoxun Wang,Xiaoming Feng,Cui Zhang,Jin Wang,Jiyan Qi,Rong Yu, and Yuling Jiao |
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2014-11-02 |
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摘要: |
Gene regulatory networks (GRNs) control development via cell type‐specific gene expression and interactions between transcription factors (TFs) and regulatory promoter regions. Plant organ boundaries separate lateral organs from the apical meristem and harbor axillary meristems (AMs). AMs, as stem cell niches, make the shoot a ramifying system. Although AMs have important functions in plant development, our knowledge of organ boundary and AM formation remains rudimentary. Here, we generated a cellular‐resolution genomewide gene expression map for low‐abundance Arabidopsis thaliana organ boundary cells and constructed a genomewide protein–DNA interaction map focusing on genes affecting boundary and AM formation. The resulting GRN uncovers transcriptional signatures, predicts cellular functions, and identifies promoter hub regions that are bound by many TFs. Importantly, further experimental studies determined the regulatory effects of many TFs on their targets, identifying regulators and regulatory relationships in AM initiation. This systems biology approach thus enhances our understanding of a key developmental process. |
英文摘要: |
Gene regulatory networks (GRNs) control development via cell type‐specific gene expression and interactions between transcription factors (TFs) and regulatory promoter regions. Plant organ boundaries separate lateral organs from the apical meristem and harbor axillary meristems (AMs). AMs, as stem cell niches, make the shoot a ramifying system. Although AMs have important functions in plant development, our knowledge of organ boundary and AM formation remains rudimentary. Here, we generated a cellular‐resolution genomewide gene expression map for low‐abundance Arabidopsis thaliana organ boundary cells and constructed a genomewide protein–DNA interaction map focusing on genes affecting boundary and AM formation. The resulting GRN uncovers transcriptional signatures, predicts cellular functions, and identifies promoter hub regions that are bound by many TFs. Importantly, further experimental studies determined the regulatory effects of many TFs on their targets, identifying regulators and regulatory relationships in AM initiation. This systems biology approach thus enhances our understanding of a key developmental process. |
刊物名称: |
Mol.Syst.Biol |
英文刊物名称: |
Mol.Syst.Biol |
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其它备注: |
Caihuan Tian,Xiaoni Zhang,Jun He,Haopeng Yu,Ying Wang,Bihai Shi,Yingying Han,Guoxun Wang,Xiaoming Feng,Cui Zhang,Jin Wang,Jiyan Qi,Rong Yu, and Yuling Jiao. An organ boundary-enriched gene regulatory network uncovers regulatory hierarchies underlying axillary meristem initiation. Mol.Syst.Biol. DOI:10.15252/msb.20145470 |
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