|
Systematically Ranking the Tightness of Membrane Association for Peripheral Membrane Proteins
Liyan Gao,Haitao Ge,Xiahe Huang,Kehui Liu,Yuanya Zhang,Wu Xu,and Yingchun Wang
Molecular and Cellular Proteomics
Abstract
Large scale quantitative evaluation of the tightness of membrane association for non-transmembrane proteins is important for identifying true peripheral membrane proteins with functional significance. Herein, we simultaneously ranked more than 1,000 proteins of the photosynthetic model organism Synechocystis sp. PCC 6803 for their relative tightness of membrane association using a proteomic approach. Using multiple precisely ranked and experimentally verified peripheral subunits of photosynthetic protein complexes as the landmarks, we found that proteins involved in two-component signal transduction systems and transporters are overall tightly associated with the membranes, whereas the associations of ribosomal proteins are much weaker. Moreover, we found that hypothetical proteins containing the same domains generally have similar tightness. This work provided a global view of the structural organization of the membrane proteome with respect to divergent functions, and built the foundation for future investigation of the dynamic membrane proteome reorganization in response to different environmental or internal stimuli.
|
论文编号: |
DOI:10.1074/mcp.M114.044800 |
论文题目: |
Systematically Ranking the Tightness of Membrane Association for Peripheral Membrane Proteins |
英文论文题目: |
Systematically Ranking the Tightness of Membrane Association for Peripheral Membrane Proteins |
第一作者: |
Liyan Gao,Haitao Ge,Xiahe Huang,Kehui Liu,Yuanya Zhang,Wu Xu,and Yingchun Wang |
英文第一作者: |
Liyan Gao,Haitao Ge,Xiahe Huang,Kehui Liu,Yuanya Zhang,Wu Xu,and Yingchun Wang |
联系作者: |
|
英文联系作者: |
|
外单位作者单位: |
|
英文外单位作者单位: |
|
发表年度: |
2014-12-16 |
卷: |
|
期: |
|
页码: |
|
摘要: |
Large scale quantitative evaluation of the tightness of membrane association for non-transmembrane proteins is important for identifying true peripheral membrane proteins with functional significance. Herein, we simultaneously ranked more than 1,000 proteins of the photosynthetic model organism Synechocystis sp. PCC 6803 for their relative tightness of membrane association using a proteomic approach. Using multiple precisely ranked and experimentally verified peripheral subunits of photosynthetic protein complexes as the landmarks, we found that proteins involved in two-component signal transduction systems and transporters are overall tightly associated with the membranes, whereas the associations of ribosomal proteins are much weaker. Moreover, we found that hypothetical proteins containing the same domains generally have similar tightness. This work provided a global view of the structural organization of the membrane proteome with respect to divergent functions, and built the foundation for future investigation of the dynamic membrane proteome reorganization in response to different environmental or internal stimuli. |
英文摘要: |
Large scale quantitative evaluation of the tightness of membrane association for non-transmembrane proteins is important for identifying true peripheral membrane proteins with functional significance. Herein, we simultaneously ranked more than 1,000 proteins of the photosynthetic model organism Synechocystis sp. PCC 6803 for their relative tightness of membrane association using a proteomic approach. Using multiple precisely ranked and experimentally verified peripheral subunits of photosynthetic protein complexes as the landmarks, we found that proteins involved in two-component signal transduction systems and transporters are overall tightly associated with the membranes, whereas the associations of ribosomal proteins are much weaker. Moreover, we found that hypothetical proteins containing the same domains generally have similar tightness. This work provided a global view of the structural organization of the membrane proteome with respect to divergent functions, and built the foundation for future investigation of the dynamic membrane proteome reorganization in response to different environmental or internal stimuli. |
刊物名称: |
Molecular and Cellular Proteomics |
英文刊物名称: |
Molecular and Cellular Proteomics |
论文全文: |
|
英文论文全文: |
|
全文链接: |
|
其它备注: |
Liyan Gao,Haitao Ge,Xiahe Huang,Kehui Liu,Yuanya Zhang,Wu Xu,and Yingchun Wang. Systematically Ranking the Tightness of Membrane Association for Peripheral Membrane Proteins. Molecular and Cellular Proteomics. DOI:10.1074/mcp.M114.044800 |
英文其它备注: |
|
学科: |
|
英文学科: |
|
影响因子: |
|
第一作者所在部门: |
|
英文第一作者所在部门: |
|
论文出处: |
|
英文论文出处: |
|
论文类别: |
|
英文论文类别: |
|
参与作者: |
|
英文参与作者: |
|
|