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Auxin depletion from leaf primordia contributes to organ patterning
Jiyan Qi,Ying Wang,Ting Yu,Alexandre Cunha,Binbin Wu,Teva Vernoux,Elliot Meyerowitz,and Yuling Jiao
PNAS
Abstract
Stem cells are responsible for organogenesis, but it is largely unknown whether and how information from stem cells acts to direct organ patterning after organ primordia are formed. It has long been proposed that the stem cells at the plant shoot apex produce a signal, which promotes leaf adaxial-abaxial (dorsoventral) patterning. Here we show the existence of a transient low auxin zone in the adaxial domain of early leaf primordia. We also demonstrate that this adaxial low auxin domain contributes to leaf adaxial-abaxial patterning. The auxin signal is mediated by the auxin-responsive transcription factor MONOPTEROS (MP), whose constitutive activation in the adaxial domain promotes abaxial cell fate. Furthermore, we show that auxin flow from emerging leaf primordia to the shoot apical meristem establishes the low auxin zone, and that this auxin flow contributes to leaf polarity. Our results provide an explanation for the hypothetical meristem-derived leaf polarity signal. Opposite to the original proposal, instead of a signal derived from the meristem, we show that a signaling molecule is departing from the primordium to the meristem to promote robustness in leaf patterning.
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论文编号: |
DOI:10.1073/pnas.1421878112 |
论文题目: |
Auxin depletion from leaf primordia contributes to organ patterning |
英文论文题目: |
Auxin depletion from leaf primordia contributes to organ patterning |
第一作者: |
Jiyan Qi,Ying Wang,Ting Yu,Alexandre Cunha,Binbin Wu,Teva Vernoux,Elliot Meyerowitz,and Yuling Jiao |
英文第一作者: |
Jiyan Qi,Ying Wang,Ting Yu,Alexandre Cunha,Binbin Wu,Teva Vernoux,Elliot Meyerowitz,and Yuling Jiao |
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2014-12-18 |
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摘要: |
Stem cells are responsible for organogenesis, but it is largely unknown whether and how information from stem cells acts to direct organ patterning after organ primordia are formed. It has long been proposed that the stem cells at the plant shoot apex produce a signal, which promotes leaf adaxial-abaxial (dorsoventral) patterning. Here we show the existence of a transient low auxin zone in the adaxial domain of early leaf primordia. We also demonstrate that this adaxial low auxin domain contributes to leaf adaxial-abaxial patterning. The auxin signal is mediated by the auxin-responsive transcription factor MONOPTEROS (MP), whose constitutive activation in the adaxial domain promotes abaxial cell fate. Furthermore, we show that auxin flow from emerging leaf primordia to the shoot apical meristem establishes the low auxin zone, and that this auxin flow contributes to leaf polarity. Our results provide an explanation for the hypothetical meristem-derived leaf polarity signal. Opposite to the original proposal, instead of a signal derived from the meristem, we show that a signaling molecule is departing from the primordium to the meristem to promote robustness in leaf patterning. |
英文摘要: |
Stem cells are responsible for organogenesis, but it is largely unknown whether and how information from stem cells acts to direct organ patterning after organ primordia are formed. It has long been proposed that the stem cells at the plant shoot apex produce a signal, which promotes leaf adaxial-abaxial (dorsoventral) patterning. Here we show the existence of a transient low auxin zone in the adaxial domain of early leaf primordia. We also demonstrate that this adaxial low auxin domain contributes to leaf adaxial-abaxial patterning. The auxin signal is mediated by the auxin-responsive transcription factor MONOPTEROS (MP), whose constitutive activation in the adaxial domain promotes abaxial cell fate. Furthermore, we show that auxin flow from emerging leaf primordia to the shoot apical meristem establishes the low auxin zone, and that this auxin flow contributes to leaf polarity. Our results provide an explanation for the hypothetical meristem-derived leaf polarity signal. Opposite to the original proposal, instead of a signal derived from the meristem, we show that a signaling molecule is departing from the primordium to the meristem to promote robustness in leaf patterning. |
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PNAS |
英文刊物名称: |
PNAS |
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其它备注: |
Jiyan Qi,Ying Wang,Ting Yu,Alexandre Cunha,Binbin Wu,Teva Vernoux,Elliot Meyerowitz,and Yuling Jiao. Auxin depletion from leaf primordia contributes to organ patterning. PNAS. DOI:10.1073/pnas.1421878112 |
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