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A Truncated NLR Protein,TIR-NBS2,Is Required for Activated Defense Responses in the exo70B1 Mutant
Ting Zhao,Lu Rui,Juan Li,Marc T. Nishimura,John P. Vogel,Na Liu,Simu Liu,Yaofei Zhao,Jeffery L. Dangl and Dingzhong Tang
PLOS GENETICS
Abstract
During exocytosis, the evolutionarily conserved exocyst complex tethers Golgi-derived vesicles to the target plasma membrane, a critical function for secretory pathways. Here we show thatexo70B1 loss-of-function mutants express activated defense responses upon infection and express enhanced resistance to fungal, oomycete and bacterial pathogens. In a screen for mutants that suppress exo70B1 resistance, we identified nine alleles of TIR-NBS2 (TN2), suggesting that loss-of-function of EXO70B1 leads to activation of this nucleotide binding domain and leucine-rich repeat-containing (NLR)-like disease resistance protein. This NLR-like protein is atypical because it lacks the LRR domain common in typical NLR receptors. In addition, we show that TN2 interacts with EXO70B1 in yeast and in planta. Our study thus provides a link between the exocyst complex and the function of a ‘TIR-NBS only’ immune receptor like protein. Our data are consistent with a speculative model wherein pathogen effectors could evolve to target EXO70B1 to manipulate plant secretion machinery. TN2 could monitor EXO70B1 integrity as part of an immune receptor complex.
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论文编号: |
DOI:10.1371/journal.pgen.1004945 |
论文题目: |
A Truncated NLR Protein,TIR-NBS2,Is Required for Activated Defense Responses in the exo70B1 Mutant |
英文论文题目: |
A Truncated NLR Protein,TIR-NBS2,Is Required for Activated Defense Responses in the exo70B1 Mutant |
第一作者: |
Ting Zhao,Lu Rui,Juan Li,Marc T. Nishimura,John P. Vogel,Na Liu,Simu Liu,Yaofei Zhao,Jeffery L. Dangl and Dingzhong Tang |
英文第一作者: |
Ting Zhao,Lu Rui,Juan Li,Marc T. Nishimura,John P. Vogel,Na Liu,Simu Liu,Yaofei Zhao,Jeffery L. Dangl and Dingzhong Tang |
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2015-01-27 |
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摘要: |
During exocytosis, the evolutionarily conserved exocyst complex tethers Golgi-derived vesicles to the target plasma membrane, a critical function for secretory pathways. Here we show thatexo70B1 loss-of-function mutants express activated defense responses upon infection and express enhanced resistance to fungal, oomycete and bacterial pathogens. In a screen for mutants that suppress exo70B1 resistance, we identified nine alleles of TIR-NBS2 (TN2), suggesting that loss-of-function of EXO70B1 leads to activation of this nucleotide binding domain and leucine-rich repeat-containing (NLR)-like disease resistance protein. This NLR-like protein is atypical because it lacks the LRR domain common in typical NLR receptors. In addition, we show that TN2 interacts with EXO70B1 in yeast and in planta. Our study thus provides a link between the exocyst complex and the function of a ‘TIR-NBS only’ immune receptor like protein. Our data are consistent with a speculative model wherein pathogen effectors could evolve to target EXO70B1 to manipulate plant secretion machinery. TN2 could monitor EXO70B1 integrity as part of an immune receptor complex. |
英文摘要: |
During exocytosis, the evolutionarily conserved exocyst complex tethers Golgi-derived vesicles to the target plasma membrane, a critical function for secretory pathways. Here we show thatexo70B1 loss-of-function mutants express activated defense responses upon infection and express enhanced resistance to fungal, oomycete and bacterial pathogens. In a screen for mutants that suppress exo70B1 resistance, we identified nine alleles of TIR-NBS2 (TN2), suggesting that loss-of-function of EXO70B1 leads to activation of this nucleotide binding domain and leucine-rich repeat-containing (NLR)-like disease resistance protein. This NLR-like protein is atypical because it lacks the LRR domain common in typical NLR receptors. In addition, we show that TN2 interacts with EXO70B1 in yeast and in planta. Our study thus provides a link between the exocyst complex and the function of a ‘TIR-NBS only’ immune receptor like protein. Our data are consistent with a speculative model wherein pathogen effectors could evolve to target EXO70B1 to manipulate plant secretion machinery. TN2 could monitor EXO70B1 integrity as part of an immune receptor complex. |
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PLOS GENETICS |
英文刊物名称: |
PLOS GENETICS |
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其它备注: |
Ting Zhao,Lu Rui,Juan Li,Marc T. Nishimura,John P. Vogel,Na Liu,Simu Liu,Yaofei Zhao,Jeffery L. Dangl and Dingzhong Tang. A Truncated NLR Protein,TIR-NBS2,Is Required for Activated Defense Responses in the exo70B1 Mutant. PLOS GENETICS. DOI:10.1371/journal.pgen.1004945 |
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