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The Alfin-like homeodomain finger protein AL5 suppresses multiple negative factors to confer abiotic stress tolerance in Arabidopsis
Wei Wei,Yu-Qin Zhang,Jian-Jun Tao,Hao-Wei Chen,Qing-Tian Li,Wan-Ke Zhang,Biao Ma,Qing Lin,Jin-Song Zhang and Shou-Yi Chen
The Plant Journal
Abstract
Plant homeodomain (PHD) finger proteins affect processes of growth and development by changing transcription and reading epigenetic modifications of histones, but their functions in abiotic stress responses remain largely unclear. Here we characterized seven Arabidopsis thaliana Alfin1-like PHD finger proteins (ALs) in the response to abiotic stresses. ALs localized to the nucleus and repressed transcription. Except AL6, all the ALs bound to G-rich element. Natural alterations of the amino acids at positions 34 and 35 in AL6 caused loss of G-rich element binding ability. ALs expressions responded differentially to osmotic stress, salt, cold and abscisic acid treatments. AL5-overexpressing plants showed higher tolerance to salt, drought and freezing stress than Col-0. Consistently, al5 mutants showed reduced stress tolerance. We used ChIP-Seq assays to identify eight direct targets of AL5 and found that AL5 can bind to the promoter regions of these genes. Knock-out mutants of five of these target genes exhibited different tolerances to stresses. These results indicate that AL5 inhibits multiple signaling pathways to confer stress tolerance. Our study sheds light on mechanisms of AL5-mediated signaling in abiotic stress responses and provides tools for improvement of stress tolerance in crop plants. This article is protected by copyright. All rights reserved. Keywords:Alfin1;Plant homeodomain;Arabidopsis;abiotic stress;ChIP-Seq
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论文编号: |
DOI:10.1111/tpj.12773 |
论文题目: |
The Alfin-like homeodomain finger protein AL5 suppresses multiple negative factors to confer abiotic stress tolerance in Arabidopsis |
英文论文题目: |
The Alfin-like homeodomain finger protein AL5 suppresses multiple negative factors to confer abiotic stress tolerance in Arabidopsis |
第一作者: |
Wei Wei,Yu-Qin Zhang,Jian-Jun Tao,Hao-Wei Chen,Qing-Tian Li,Wan-Ke Zhang,Biao Ma,Qing Lin,Jin-Song Zhang and Shou-Yi Chen |
英文第一作者: |
Wei Wei,Yu-Qin Zhang,Jian-Jun Tao,Hao-Wei Chen,Qing-Tian Li,Wan-Ke Zhang,Biao Ma,Qing Lin,Jin-Song Zhang and Shou-Yi Chen |
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2015-02-04 |
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摘要: |
Plant homeodomain (PHD) finger proteins affect processes of growth and development by changing transcription and reading epigenetic modifications of histones, but their functions in abiotic stress responses remain largely unclear. Here we characterized seven Arabidopsis thaliana Alfin1-like PHD finger proteins (ALs) in the response to abiotic stresses. ALs localized to the nucleus and repressed transcription. Except AL6, all the ALs bound to G-rich element. Natural alterations of the amino acids at positions 34 and 35 in AL6 caused loss of G-rich element binding ability. ALs expressions responded differentially to osmotic stress, salt, cold and abscisic acid treatments. AL5-overexpressing plants showed higher tolerance to salt, drought and freezing stress than Col-0. Consistently, al5 mutants showed reduced stress tolerance. We used ChIP-Seq assays to identify eight direct targets of AL5 and found that AL5 can bind to the promoter regions of these genes. Knock-out mutants of five of these target genes exhibited different tolerances to stresses. These results indicate that AL5 inhibits multiple signaling pathways to confer stress tolerance. Our study sheds light on mechanisms of AL5-mediated signaling in abiotic stress responses and provides tools for improvement of stress tolerance in crop plants. This article is protected by copyright. All rights reserved. Keywords:Alfin1;Plant homeodomain;Arabidopsis;abiotic stress;ChIP-Seq |
英文摘要: |
Plant homeodomain (PHD) finger proteins affect processes of growth and development by changing transcription and reading epigenetic modifications of histones, but their functions in abiotic stress responses remain largely unclear. Here we characterized seven Arabidopsis thaliana Alfin1-like PHD finger proteins (ALs) in the response to abiotic stresses. ALs localized to the nucleus and repressed transcription. Except AL6, all the ALs bound to G-rich element. Natural alterations of the amino acids at positions 34 and 35 in AL6 caused loss of G-rich element binding ability. ALs expressions responded differentially to osmotic stress, salt, cold and abscisic acid treatments. AL5-overexpressing plants showed higher tolerance to salt, drought and freezing stress than Col-0. Consistently, al5 mutants showed reduced stress tolerance. We used ChIP-Seq assays to identify eight direct targets of AL5 and found that AL5 can bind to the promoter regions of these genes. Knock-out mutants of five of these target genes exhibited different tolerances to stresses. These results indicate that AL5 inhibits multiple signaling pathways to confer stress tolerance. Our study sheds light on mechanisms of AL5-mediated signaling in abiotic stress responses and provides tools for improvement of stress tolerance in crop plants. This article is protected by copyright. All rights reserved. Keywords:Alfin1;Plant homeodomain;Arabidopsis;abiotic stress;ChIP-Seq |
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The Plant Journal |
英文刊物名称: |
The Plant Journal |
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其它备注: |
Wei Wei,Yu-Qin Zhang,Jian-Jun Tao,Hao-Wei Chen,Qing-Tian Li,Wan-Ke Zhang,Biao Ma,Qing Lin,Jin-Song Zhang and Shou-Yi Chen. The Alfin-like homeodomain finger protein AL5 suppresses multiple negative factors to confer abiotic stress tolerance in Arabidopsis. The Plant Journal. DOI:10.1111/tpj.12773 |
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