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Functional Evolution of Phosphatidylethanolamine Binding Proteins in Soybean and Arabidopsis
Zheng Wang,Zhengkui Zhou,Yunfeng Liu,Tengfei Liu,Qing Li,Yuanyuan Ji,Congcong Li,Chao Fang,Min Wang,Mian Wu,Yanting Shen,Tian Tang,Jianxin Ma, and Zhixi Tian
The Plant Cell
Abstract
Gene duplication provides resources for novel gene functions. Identification of the amino acids responsible for functional conservation and divergence of duplicated genes will strengthen our understanding of their evolutionary course. Here, we conducted a systemic functional investigation of phosphatidylethanolamine binding proteins (PEBPs) in soybean (Glycine max) andArabidopsis thaliana. Our results demonstrated that after the ancestral duplication, the lineage of the common ancestor of the FLOWERING LOCUS T (FT) and TERMINAL FLOWER1 (TFL1) subfamilies functionally diverged from theMOTHER OF FT AND TFL1 (MFT) subfamily to activate flowering and repress flowering, respectively. They also underwent further specialization after subsequent duplications. Although the functional divergence increased with duplication age, we observed rapid functional divergence for a few pairs of young duplicates in soybean. Association analysis between amino acids and functional variations identified critical amino acid residues that led to functional differences in PEBP members. Using transgenic analysis, we validated a subset of these differences. We report clear experimental evidence for the functional evolution of the PEBPs in the MFT, FT, and TFL1 subfamilies, which predate the origin of angiosperms. Our results highlight the role of amino acid divergence in driving evolutionary novelty after duplication.
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DOI:10.1105/tpc.114.135103 |
论文题目: |
Functional Evolution of Phosphatidylethanolamine Binding Proteins in Soybean and Arabidopsis |
英文论文题目: |
Functional Evolution of Phosphatidylethanolamine Binding Proteins in Soybean and Arabidopsis |
第一作者: |
Zheng Wang,Zhengkui Zhou,Yunfeng Liu,Tengfei Liu,Qing Li,Yuanyuan Ji,Congcong Li,Chao Fang,Min Wang,Mian Wu,Yanting Shen,Tian Tang,Jianxin Ma, and Zhixi Tian |
英文第一作者: |
Zheng Wang,Zhengkui Zhou,Yunfeng Liu,Tengfei Liu,Qing Li,Yuanyuan Ji,Congcong Li,Chao Fang,Min Wang,Mian Wu,Yanting Shen,Tian Tang,Jianxin Ma, and Zhixi Tian |
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2015-02-09 |
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摘要: |
Gene duplication provides resources for novel gene functions. Identification of the amino acids responsible for functional conservation and divergence of duplicated genes will strengthen our understanding of their evolutionary course. Here, we conducted a systemic functional investigation of phosphatidylethanolamine binding proteins (PEBPs) in soybean (Glycine max) andArabidopsis thaliana. Our results demonstrated that after the ancestral duplication, the lineage of the common ancestor of the FLOWERING LOCUS T (FT) and TERMINAL FLOWER1 (TFL1) subfamilies functionally diverged from theMOTHER OF FT AND TFL1 (MFT) subfamily to activate flowering and repress flowering, respectively. They also underwent further specialization after subsequent duplications. Although the functional divergence increased with duplication age, we observed rapid functional divergence for a few pairs of young duplicates in soybean. Association analysis between amino acids and functional variations identified critical amino acid residues that led to functional differences in PEBP members. Using transgenic analysis, we validated a subset of these differences. We report clear experimental evidence for the functional evolution of the PEBPs in the MFT, FT, and TFL1 subfamilies, which predate the origin of angiosperms. Our results highlight the role of amino acid divergence in driving evolutionary novelty after duplication. |
英文摘要: |
Gene duplication provides resources for novel gene functions. Identification of the amino acids responsible for functional conservation and divergence of duplicated genes will strengthen our understanding of their evolutionary course. Here, we conducted a systemic functional investigation of phosphatidylethanolamine binding proteins (PEBPs) in soybean (Glycine max) andArabidopsis thaliana. Our results demonstrated that after the ancestral duplication, the lineage of the common ancestor of the FLOWERING LOCUS T (FT) and TERMINAL FLOWER1 (TFL1) subfamilies functionally diverged from theMOTHER OF FT AND TFL1 (MFT) subfamily to activate flowering and repress flowering, respectively. They also underwent further specialization after subsequent duplications. Although the functional divergence increased with duplication age, we observed rapid functional divergence for a few pairs of young duplicates in soybean. Association analysis between amino acids and functional variations identified critical amino acid residues that led to functional differences in PEBP members. Using transgenic analysis, we validated a subset of these differences. We report clear experimental evidence for the functional evolution of the PEBPs in the MFT, FT, and TFL1 subfamilies, which predate the origin of angiosperms. Our results highlight the role of amino acid divergence in driving evolutionary novelty after duplication. |
刊物名称: |
The Plant Cell |
英文刊物名称: |
The Plant Cell |
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其它备注: |
Zheng Wang,Zhengkui Zhou,Yunfeng Liu,Tengfei Liu,Qing Li,Yuanyuan Ji,Congcong Li,Chao Fang,Min Wang,Mian Wu,Yanting Shen,Tian Tang,Jianxin Ma, and Zhixi Tian. Functional Evolution of Phosphatidylethanolamine Binding Proteins in Soybean and Arabidopsis. The Plant Cell. DOI:10.1105/tpc.114.135103 |
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