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Impaired maturation of large dense core vesicles in muted-deficient adrenal chromaffin cells
Zhenhua Hao,Lisi Wei,Yaqin Feng,Xiaowei Chen,Wen Du,Jing Ma,Zhuan Zhou,Liangyi Chen and Wei Li
Jouranl of Cell Science
Abstract
The large dense-core vesicle (LDCV), a type of lysosome-related organelle, is involved in the secretion of hormones and neuropeptides in specialized secretory cells. The granin family is a driving force in LDCV biogenesis, but the machinery for granin sorting to this biogenesis pathway is largely unknown. The mu mutant mouse, which carries a spontaneous null mutation on the Muted gene (also known as Bloc1s5) that encodes a subunit of lysosome-related organelles complex-1 (BLOC-1), is a mouse model of Hermansky-Pudlak syndrome. We here found that LDCVs were enlarged in muadrenal chromaffin cells. Chromogranin A (CgA) was increased in mu adrenals and muted-knockdown cells. The increased CgA in mu mice was likely due to the failure of its sorting-out, which impairs LDCV maturation and docking. Inmu chromaffin cells, the size of readily releasable pool and the vesicle release frequency were reduced. Our studies suggest that the muted protein is involved in the sorting-out of CgA during the biogenesis of LDCVs.
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论文编号: |
DOI:10.1242/jcs.161414 |
论文题目: |
Impaired maturation of large dense core vesicles in muted-deficient adrenal chromaffin cells |
英文论文题目: |
Impaired maturation of large dense core vesicles in muted-deficient adrenal chromaffin cells |
第一作者: |
Zhenhua Hao,Lisi Wei,Yaqin Feng,Xiaowei Chen,Wen Du,Jing Ma,Zhuan Zhou,Liangyi Chen and Wei Li |
英文第一作者: |
Zhenhua Hao,Lisi Wei,Yaqin Feng,Xiaowei Chen,Wen Du,Jing Ma,Zhuan Zhou,Liangyi Chen and Wei Li |
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2015-02-13 |
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摘要: |
The large dense-core vesicle (LDCV), a type of lysosome-related organelle, is involved in the secretion of hormones and neuropeptides in specialized secretory cells. The granin family is a driving force in LDCV biogenesis, but the machinery for granin sorting to this biogenesis pathway is largely unknown. The mu mutant mouse, which carries a spontaneous null mutation on the Muted gene (also known as Bloc1s5) that encodes a subunit of lysosome-related organelles complex-1 (BLOC-1), is a mouse model of Hermansky-Pudlak syndrome. We here found that LDCVs were enlarged in muadrenal chromaffin cells. Chromogranin A (CgA) was increased in mu adrenals and muted-knockdown cells. The increased CgA in mu mice was likely due to the failure of its sorting-out, which impairs LDCV maturation and docking. Inmu chromaffin cells, the size of readily releasable pool and the vesicle release frequency were reduced. Our studies suggest that the muted protein is involved in the sorting-out of CgA during the biogenesis of LDCVs. |
英文摘要: |
The large dense-core vesicle (LDCV), a type of lysosome-related organelle, is involved in the secretion of hormones and neuropeptides in specialized secretory cells. The granin family is a driving force in LDCV biogenesis, but the machinery for granin sorting to this biogenesis pathway is largely unknown. The mu mutant mouse, which carries a spontaneous null mutation on the Muted gene (also known as Bloc1s5) that encodes a subunit of lysosome-related organelles complex-1 (BLOC-1), is a mouse model of Hermansky-Pudlak syndrome. We here found that LDCVs were enlarged in muadrenal chromaffin cells. Chromogranin A (CgA) was increased in mu adrenals and muted-knockdown cells. The increased CgA in mu mice was likely due to the failure of its sorting-out, which impairs LDCV maturation and docking. Inmu chromaffin cells, the size of readily releasable pool and the vesicle release frequency were reduced. Our studies suggest that the muted protein is involved in the sorting-out of CgA during the biogenesis of LDCVs. |
刊物名称: |
Jouranl of Cell Science |
英文刊物名称: |
Jouranl of Cell Science |
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其它备注: |
Zhenhua Hao,Lisi Wei,Yaqin Feng,Xiaowei Chen,Wen Du,Jing Ma,Zhuan Zhou,Liangyi Chen and Wei Li. Impaired maturation of large dense core vesicles in muted-deficient adrenal chromaffin cells. Jouranl of Cell Science. DOI:10.1242/jcs.161414 |
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