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The RING finger E3 ligase STRF1 is involved in membrane trafficking and modulates salt stress response in Arabidopsis thaliana
Miaomiao Tian,Lijuan Lou,Lijing Liu,Feifei Yu,Qingzhen Zhao,Huawei Zhang,Yaorong Wu,Sanyuan Tang,Ran Xia,Baoge Zhu,Giovanna Serino and Qi Xie
The Plant Journal
Abstract
Keywords: STRF1;membrane trafficking;salt stress;ubiquitination Summary Salt stress is a detrimental factor for plant growth and development. The response to salt stress has been shown to involve components in the intracellular trafficking system, as well as components of the ubiquitin-proteasome system (UPS). In this article, we have identified in Arabidopsis thaliana a little reported ubiquitin ligase involved in salt stress response, which we named STRF1 (Salt Tolerance RING Finger 1). STRF1 is a member of RING-H2 finger proteins and we demonstrate that it has ubiquitin ligase activity in vitro. We also show that STRF1 localizes mainly at the plasma membrane and at the intracellular endosomes. strf1-1 loss of function mutant seedlings exhibit accelerated endocytosis in roots, and have altered expression of several genes involved in the membrane trafficking system. Moreover, protein trafficking inhibitor, BFA treatment has increased BFA bodies in strf1-1 mutant. This mutant also showed increased tolerance to salt, ionic and osmotic stresses, reduced accumulation of reactive oxygen species during salt stress, and increased expression of AtRbohD, which encodes a NADPH oxidase involved in H2O2 production. We conclude that STRF1 is a membrane trafficking-related ubiquitin ligase, which helps the plant to respond to salt stress by monitoring intracellular membrane trafficking and ROS production. This article is protected by copyright. All rights reserved.
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DOI:10.1111/tpj.12797 |
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The RING finger E3 ligase STRF1 is involved in membrane trafficking and modulates salt stress response in Arabidopsis thaliana |
英文论文题目: |
The RING finger E3 ligase STRF1 is involved in membrane trafficking and modulates salt stress response in Arabidopsis thaliana |
第一作者: |
Miaomiao Tian,Lijuan Lou,Lijing Liu,Feifei Yu,Qingzhen Zhao,Huawei Zhang,Yaorong Wu,Sanyuan Tang,Ran Xia,Baoge Zhu,Giovanna Serino and Qi Xie |
英文第一作者: |
Miaomiao Tian,Lijuan Lou,Lijing Liu,Feifei Yu,Qingzhen Zhao,Huawei Zhang,Yaorong Wu,Sanyuan Tang,Ran Xia,Baoge Zhu,Giovanna Serino and Qi Xie |
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2015-03-01 |
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Keywords: STRF1;membrane trafficking;salt stress;ubiquitination Summary Salt stress is a detrimental factor for plant growth and development. The response to salt stress has been shown to involve components in the intracellular trafficking system, as well as components of the ubiquitin-proteasome system (UPS). In this article, we have identified in Arabidopsis thaliana a little reported ubiquitin ligase involved in salt stress response, which we named STRF1 (Salt Tolerance RING Finger 1). STRF1 is a member of RING-H2 finger proteins and we demonstrate that it has ubiquitin ligase activity in vitro. We also show that STRF1 localizes mainly at the plasma membrane and at the intracellular endosomes. strf1-1 loss of function mutant seedlings exhibit accelerated endocytosis in roots, and have altered expression of several genes involved in the membrane trafficking system. Moreover, protein trafficking inhibitor, BFA treatment has increased BFA bodies in strf1-1 mutant. This mutant also showed increased tolerance to salt, ionic and osmotic stresses, reduced accumulation of reactive oxygen species during salt stress, and increased expression of AtRbohD, which encodes a NADPH oxidase involved in H2O2 production. We conclude that STRF1 is a membrane trafficking-related ubiquitin ligase, which helps the plant to respond to salt stress by monitoring intracellular membrane trafficking and ROS production. This article is protected by copyright. All rights reserved. |
英文摘要: |
Keywords: STRF1;membrane trafficking;salt stress;ubiquitination Summary Salt stress is a detrimental factor for plant growth and development. The response to salt stress has been shown to involve components in the intracellular trafficking system, as well as components of the ubiquitin-proteasome system (UPS). In this article, we have identified in Arabidopsis thaliana a little reported ubiquitin ligase involved in salt stress response, which we named STRF1 (Salt Tolerance RING Finger 1). STRF1 is a member of RING-H2 finger proteins and we demonstrate that it has ubiquitin ligase activity in vitro. We also show that STRF1 localizes mainly at the plasma membrane and at the intracellular endosomes. strf1-1 loss of function mutant seedlings exhibit accelerated endocytosis in roots, and have altered expression of several genes involved in the membrane trafficking system. Moreover, protein trafficking inhibitor, BFA treatment has increased BFA bodies in strf1-1 mutant. This mutant also showed increased tolerance to salt, ionic and osmotic stresses, reduced accumulation of reactive oxygen species during salt stress, and increased expression of AtRbohD, which encodes a NADPH oxidase involved in H2O2 production. We conclude that STRF1 is a membrane trafficking-related ubiquitin ligase, which helps the plant to respond to salt stress by monitoring intracellular membrane trafficking and ROS production. This article is protected by copyright. All rights reserved. |
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The Plant Journal |
英文刊物名称: |
The Plant Journal |
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其它备注: |
Miaomiao Tian,Lijuan Lou,Lijing Liu,Feifei Yu,Qingzhen Zhao,Huawei Zhang,Yaorong Wu,Sanyuan Tang,Ran Xia,Baoge Zhu,Giovanna Serino and Qi Xie. The RING finger E3 ligase STRF1 is involved in membrane trafficking and modulates salt stress response in Arabidopsis thaliana. The Plant Journal. DOI:10.1111/tpj.12797 |
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