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The C.elegans COE transcription factor UNC-3 activates lineage-specific apoptosis and affects neurite growth in the RID lineage
Jinbo Wang,Jyothsna Chitturi,Qinglan Ge,Valeriya Laskova,Wei Wang,Xia Li,Mei Ding,Mei Zhen,and Xun Huang
Development
Abstract
Mechanisms that regulate apoptosis in a temporal and lineage-specific manner remain poorly understood. The COE (Collier/Olf/EBF) transcription factors have been implicated in the development of many cell types, including neurons. Here, we show that the soleCaenorhabditis elegans COE protein, UNC-3, together with a histone acetyltransferase, CBP-1/P300, specifies lineage-specific apoptosis and certain aspects of neurite trajectory. During embryogenesis, the RID progenitor cell gives rise to the RID neuron and RID sister cell; the latter undergoes apoptosis shortly after cell division upon expression of the pro-apoptotic gene egl-1. We observe UNC-3 expression in the RID progenitor, and the absence of UNC-3 results in the failure of the RID lineage to express a Pegl-1::GFP reporter and in the survival of the RID sister cell. Lastly, UNC-3 interacts with CBP-1, and cbp-1 mutants exhibit a similar RID phenotype to unc-3. Thus, in addition to playing a role in neuronal terminal differentiation, UNC-3 is a cell lineage-specific regulator of apoptosis.
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DOI:10.1242/dev.119479 |
论文题目: |
The C.elegans COE transcription factor UNC-3 activates lineage-specific apoptosis and affects neurite growth in the RID lineage |
英文论文题目: |
The C.elegans COE transcription factor UNC-3 activates lineage-specific apoptosis and affects neurite growth in the RID lineage |
第一作者: |
Jinbo Wang,Jyothsna Chitturi,Qinglan Ge,Valeriya Laskova,Wei Wang,Xia Li,Mei Ding,Mei Zhen,and Xun Huang |
英文第一作者: |
Jinbo Wang,Jyothsna Chitturi,Qinglan Ge,Valeriya Laskova,Wei Wang,Xia Li,Mei Ding,Mei Zhen,and Xun Huang |
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2015-03-27 |
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摘要: |
Mechanisms that regulate apoptosis in a temporal and lineage-specific manner remain poorly understood. The COE (Collier/Olf/EBF) transcription factors have been implicated in the development of many cell types, including neurons. Here, we show that the soleCaenorhabditis elegans COE protein, UNC-3, together with a histone acetyltransferase, CBP-1/P300, specifies lineage-specific apoptosis and certain aspects of neurite trajectory. During embryogenesis, the RID progenitor cell gives rise to the RID neuron and RID sister cell; the latter undergoes apoptosis shortly after cell division upon expression of the pro-apoptotic gene egl-1. We observe UNC-3 expression in the RID progenitor, and the absence of UNC-3 results in the failure of the RID lineage to express a Pegl-1::GFP reporter and in the survival of the RID sister cell. Lastly, UNC-3 interacts with CBP-1, and cbp-1 mutants exhibit a similar RID phenotype to unc-3. Thus, in addition to playing a role in neuronal terminal differentiation, UNC-3 is a cell lineage-specific regulator of apoptosis. |
英文摘要: |
Mechanisms that regulate apoptosis in a temporal and lineage-specific manner remain poorly understood. The COE (Collier/Olf/EBF) transcription factors have been implicated in the development of many cell types, including neurons. Here, we show that the soleCaenorhabditis elegans COE protein, UNC-3, together with a histone acetyltransferase, CBP-1/P300, specifies lineage-specific apoptosis and certain aspects of neurite trajectory. During embryogenesis, the RID progenitor cell gives rise to the RID neuron and RID sister cell; the latter undergoes apoptosis shortly after cell division upon expression of the pro-apoptotic gene egl-1. We observe UNC-3 expression in the RID progenitor, and the absence of UNC-3 results in the failure of the RID lineage to express a Pegl-1::GFP reporter and in the survival of the RID sister cell. Lastly, UNC-3 interacts with CBP-1, and cbp-1 mutants exhibit a similar RID phenotype to unc-3. Thus, in addition to playing a role in neuronal terminal differentiation, UNC-3 is a cell lineage-specific regulator of apoptosis. |
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Development |
英文刊物名称: |
Development |
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其它备注: |
Jinbo Wang,Jyothsna Chitturi,Qinglan Ge,Valeriya Laskova,Wei Wang,Xia Li,Mei Ding,Mei Zhen,and Xun Huang. The C.elegans COE transcription factor UNC-3 activates lineage-specific apoptosis and affects neurite growth in the RID lineage. Development. DOI:10.1242/dev.119479 |
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