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Functional disruption of the dystrophin gene in Rhesus Monkey Using CRISPR/Cas9
Yongchang Chen,Yinghui Zheng,Yu Kang,Weili Yang,Yuyu Niu,Xiangyu Guo,Zhuchi Tu,Chenyang Si,Hong Wang,Ruxiao Xing,Xiuqiong Pu,Shang-Hsun Yang,Shihua Li,Weizhi Ji and Xiao-Jiang Li
Human Molecular Genetics
Abstract
CRISPR/Cas9 has been used to genetically modify genomes in a variety of species, including non-human primates. Unfortunately, this new technology does cause mosaic mutations, and we do not yet know whether such mutations can functionally disrupt the targeted gene, or cause the pathology seen in human disease. Addressing these issues is necessary if we are to generate large animal models of human diseases using CRISPR/Cas9. Here we used CRISPR/Cas9 to target the monkey dystrophin gene to create mutations that lead to Duchenne muscular dystrophy (DMD), a recessive X-linked form of muscular dystrophy. Examination of the relative targeting rate revealed that Crispr/Cas9 targeting could lead to mosaic mutations in up to 87% of the dystrophin alleles in monkey muscle. Moreover, CRISPR/Cas9 induced mutations in both male and female monkeys, with the markedly depleted dystrophin and muscle degeneration seen in early DMD. Our findings indicate that CRISPR/Cas9 can efficiently generate monkey models of human diseases, regardless of inheritance patterns. The presence of degenerated muscle cells in newborn Cas9-targeted monkeys suggests that therapeutic interventions at the early disease stage may be effective at alleviating the myopathy.
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DOI:10.1093/hmg/ddv120 |
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Functional disruption of the dystrophin gene in Rhesus Monkey Using CRISPR/Cas9 |
英文论文题目: |
Functional disruption of the dystrophin gene in Rhesus Monkey Using CRISPR/Cas9 |
第一作者: |
Yongchang Chen,Yinghui Zheng,Yu Kang,Weili Yang,Yuyu Niu,Xiangyu Guo,Zhuchi Tu,Chenyang Si,Hong Wang,Ruxiao Xing,Xiuqiong Pu,Shang-Hsun Yang,Shihua Li,Weizhi Ji and Xiao-Jiang Li |
英文第一作者: |
Yongchang Chen,Yinghui Zheng,Yu Kang,Weili Yang,Yuyu Niu,Xiangyu Guo,Zhuchi Tu,Chenyang Si,Hong Wang,Ruxiao Xing,Xiuqiong Pu,Shang-Hsun Yang,Shihua Li,Weizhi Ji and Xiao-Jiang Li |
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2015-04-15 |
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摘要: |
CRISPR/Cas9 has been used to genetically modify genomes in a variety of species, including non-human primates. Unfortunately, this new technology does cause mosaic mutations, and we do not yet know whether such mutations can functionally disrupt the targeted gene, or cause the pathology seen in human disease. Addressing these issues is necessary if we are to generate large animal models of human diseases using CRISPR/Cas9. Here we used CRISPR/Cas9 to target the monkey dystrophin gene to create mutations that lead to Duchenne muscular dystrophy (DMD), a recessive X-linked form of muscular dystrophy. Examination of the relative targeting rate revealed that Crispr/Cas9 targeting could lead to mosaic mutations in up to 87% of the dystrophin alleles in monkey muscle. Moreover, CRISPR/Cas9 induced mutations in both male and female monkeys, with the markedly depleted dystrophin and muscle degeneration seen in early DMD. Our findings indicate that CRISPR/Cas9 can efficiently generate monkey models of human diseases, regardless of inheritance patterns. The presence of degenerated muscle cells in newborn Cas9-targeted monkeys suggests that therapeutic interventions at the early disease stage may be effective at alleviating the myopathy. |
英文摘要: |
CRISPR/Cas9 has been used to genetically modify genomes in a variety of species, including non-human primates. Unfortunately, this new technology does cause mosaic mutations, and we do not yet know whether such mutations can functionally disrupt the targeted gene, or cause the pathology seen in human disease. Addressing these issues is necessary if we are to generate large animal models of human diseases using CRISPR/Cas9. Here we used CRISPR/Cas9 to target the monkey dystrophin gene to create mutations that lead to Duchenne muscular dystrophy (DMD), a recessive X-linked form of muscular dystrophy. Examination of the relative targeting rate revealed that Crispr/Cas9 targeting could lead to mosaic mutations in up to 87% of the dystrophin alleles in monkey muscle. Moreover, CRISPR/Cas9 induced mutations in both male and female monkeys, with the markedly depleted dystrophin and muscle degeneration seen in early DMD. Our findings indicate that CRISPR/Cas9 can efficiently generate monkey models of human diseases, regardless of inheritance patterns. The presence of degenerated muscle cells in newborn Cas9-targeted monkeys suggests that therapeutic interventions at the early disease stage may be effective at alleviating the myopathy. |
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Human Molecular Genetics |
英文刊物名称: |
Human Molecular Genetics |
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Yongchang Chen,Yinghui Zheng,Yu Kang,Weili Yang,Yuyu Niu,Xiangyu Guo,Zhuchi Tu,Chenyang Si,Hong Wang,Ruxiao Xing,Xiuqiong Pu,Shang-Hsun Yang,Shihua Li,Weizhi Ji and Xiao-Jiang Li. Functional disruption of the dystrophin gene in Rhesus Monkey Using CRISPR/Cas9. Human Molecular Genetics. DOI:10.1093/hmg/ddv120 |
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