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A functionalized collagen scaffold neutralizing the myelin inhibitory molecules promoted neurites outgrowth in vitro and facilitated spinal cord regeneration in vivo
Xing Li,Jin Han,Yannan Zhao,Wenyong Ding,Jianshu Wei,Sufang Han,Xianping Shang,Bin Wang,Bing Chen,Zhifeng Xiao,and Jianwu Dai
ACS Applied Materials and Interfaces
Abstract
Research has demonstrated that many myelin-associated inhibitory molecules jointly contribute to the failure of adult spinal cord regeneration. Therapies comprehensively targeting the multiple inhibitory nature of the injured spinal cord are being concerned. Here, two collagen-binding proteins, CBD-EphA4LBD and CBD-PlexinB1LBD were constructed respectively to neutralize the axon guidance molecules ephrinB3 and sema4D which inhibit the regeneration of nerve fibers. The two neutralizing proteins have been proven their ability to specifically bind the collagen and to continuously release from collagen scaffolds. They could also promote neurites outgrowth of cerebellar granular neurons and dorsal root ganglion neurons in vitro. Subsequently, the functionalized collagen scaffolds by physically absorbing NEP1-40 and immobilizing CBD-EphA4LBD and CBD-PlexinB1LBD were transplanted into a rat T10 complete spinal cord transection model. Our results showed that rats received the treatment of transplanting the functionalized collagen scaffold exhibited great advantage on axonal regeneration and locomotion recovery after spinal cord injury.
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论文编号: |
DOI:10.1021/acsami.5b03879 |
论文题目: |
A functionalized collagen scaffold neutralizing the myelin inhibitory molecules promoted neurites outgrowth in vitro and facilitated spinal cord regeneration in vivo |
英文论文题目: |
A functionalized collagen scaffold neutralizing the myelin inhibitory molecules promoted neurites outgrowth in vitro and facilitated spinal cord regeneration in vivo |
第一作者: |
Xing Li,Jin Han,Yannan Zhao,Wenyong Ding,Jianshu Wei,Sufang Han,Xianping Shang,Bin Wang,Bing Chen,Zhifeng Xiao,and Jianwu Dai |
英文第一作者: |
Xing Li,Jin Han,Yannan Zhao,Wenyong Ding,Jianshu Wei,Sufang Han,Xianping Shang,Bin Wang,Bing Chen,Zhifeng Xiao,and Jianwu Dai |
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2015-06-16 |
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摘要: |
Research has demonstrated that many myelin-associated inhibitory molecules jointly contribute to the failure of adult spinal cord regeneration. Therapies comprehensively targeting the multiple inhibitory nature of the injured spinal cord are being concerned. Here, two collagen-binding proteins, CBD-EphA4LBD and CBD-PlexinB1LBD were constructed respectively to neutralize the axon guidance molecules ephrinB3 and sema4D which inhibit the regeneration of nerve fibers. The two neutralizing proteins have been proven their ability to specifically bind the collagen and to continuously release from collagen scaffolds. They could also promote neurites outgrowth of cerebellar granular neurons and dorsal root ganglion neurons in vitro. Subsequently, the functionalized collagen scaffolds by physically absorbing NEP1-40 and immobilizing CBD-EphA4LBD and CBD-PlexinB1LBD were transplanted into a rat T10 complete spinal cord transection model. Our results showed that rats received the treatment of transplanting the functionalized collagen scaffold exhibited great advantage on axonal regeneration and locomotion recovery after spinal cord injury. |
英文摘要: |
Research has demonstrated that many myelin-associated inhibitory molecules jointly contribute to the failure of adult spinal cord regeneration. Therapies comprehensively targeting the multiple inhibitory nature of the injured spinal cord are being concerned. Here, two collagen-binding proteins, CBD-EphA4LBD and CBD-PlexinB1LBD were constructed respectively to neutralize the axon guidance molecules ephrinB3 and sema4D which inhibit the regeneration of nerve fibers. The two neutralizing proteins have been proven their ability to specifically bind the collagen and to continuously release from collagen scaffolds. They could also promote neurites outgrowth of cerebellar granular neurons and dorsal root ganglion neurons in vitro. Subsequently, the functionalized collagen scaffolds by physically absorbing NEP1-40 and immobilizing CBD-EphA4LBD and CBD-PlexinB1LBD were transplanted into a rat T10 complete spinal cord transection model. Our results showed that rats received the treatment of transplanting the functionalized collagen scaffold exhibited great advantage on axonal regeneration and locomotion recovery after spinal cord injury. |
刊物名称: |
ACS Applied Materials and Interfaces |
英文刊物名称: |
ACS Applied Materials and Interfaces |
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其它备注: |
Xing Li,Jin Han,Yannan Zhao,Wenyong Ding,Jianshu Wei,Sufang Han,Xianping Shang,Bin Wang,Bing Chen,Zhifeng Xiao,and Jianwu Dai. A functionalized collagen scaffold neutralizing the myelin inhibitory molecules promoted neurites outgrowth in vitro and facilitated spinal cord regeneration in vivo. ACS Applied Materials and Interfaces. DOI:10.1021/acsami.5b03879 |
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