|
CRISPR/Cas9: a powerful genetic engineering tool for establishing large animal models of neurodegenerative diseases
Zhuchi Tu,Weili Yang,Sen Yan,Xiangyu Guo and Xiao-Jiang Li
Molecular Neurodegeneration
Abstract
Animal models are extremely valuable to help us understand the pathogenesis of neurodegenerative disorders and to find treatments for them. Since large animals are more like humans than rodents, they make good models to identify the important pathological events that may be seen in humans but not in small animals; large animals are also very important for validating effective treatments or confirming therapeutic targets. Due to the lack of embryonic stem cell lines from large animals, it has been difficult to use traditional gene targeting technology to establish large animal models of neurodegenerative diseases. Recently, CRISPR/Cas9 was used successfully to genetically modify genomes in various species. Here we discuss the use of CRISPR/Cas9 technology to establish large animal models that can more faithfully mimic human neurodegenerative diseases. Keywords: CRISPR/Cas9; Non-human primates; Neurodegenerative diseases; Animal model
|
论文编号: |
DOI:10.1186/s13024-015-0031-x |
论文题目: |
CRISPR/Cas9: a powerful genetic engineering tool for establishing large animal models of neurodegenerative diseases |
英文论文题目: |
CRISPR/Cas9: a powerful genetic engineering tool for establishing large animal models of neurodegenerative diseases |
第一作者: |
Zhuchi Tu,Weili Yang,Sen Yan,Xiangyu Guo and Xiao-Jiang Li |
英文第一作者: |
Zhuchi Tu,Weili Yang,Sen Yan,Xiangyu Guo and Xiao-Jiang Li |
联系作者: |
|
英文联系作者: |
|
外单位作者单位: |
|
英文外单位作者单位: |
|
发表年度: |
2015-08-07 |
卷: |
|
期: |
|
页码: |
|
摘要: |
Animal models are extremely valuable to help us understand the pathogenesis of neurodegenerative disorders and to find treatments for them. Since large animals are more like humans than rodents, they make good models to identify the important pathological events that may be seen in humans but not in small animals; large animals are also very important for validating effective treatments or confirming therapeutic targets. Due to the lack of embryonic stem cell lines from large animals, it has been difficult to use traditional gene targeting technology to establish large animal models of neurodegenerative diseases. Recently, CRISPR/Cas9 was used successfully to genetically modify genomes in various species. Here we discuss the use of CRISPR/Cas9 technology to establish large animal models that can more faithfully mimic human neurodegenerative diseases. Keywords: CRISPR/Cas9; Non-human primates; Neurodegenerative diseases; Animal model |
英文摘要: |
Animal models are extremely valuable to help us understand the pathogenesis of neurodegenerative disorders and to find treatments for them. Since large animals are more like humans than rodents, they make good models to identify the important pathological events that may be seen in humans but not in small animals; large animals are also very important for validating effective treatments or confirming therapeutic targets. Due to the lack of embryonic stem cell lines from large animals, it has been difficult to use traditional gene targeting technology to establish large animal models of neurodegenerative diseases. Recently, CRISPR/Cas9 was used successfully to genetically modify genomes in various species. Here we discuss the use of CRISPR/Cas9 technology to establish large animal models that can more faithfully mimic human neurodegenerative diseases. Keywords: CRISPR/Cas9; Non-human primates; Neurodegenerative diseases; Animal model |
刊物名称: |
Molecular Neurodegeneration |
英文刊物名称: |
Molecular Neurodegeneration |
论文全文: |
|
英文论文全文: |
|
全文链接: |
|
其它备注: |
Zhuchi Tu,Weili Yang,Sen Yan,Xiangyu Guo and Xiao-Jiang Li. CRISPR/Cas9: a powerful genetic engineering tool for establishing large animal models of neurodegenerative diseases. Molecular Neurodegeneration. DOI:10.1186/s13024-015-0031-x |
英文其它备注: |
|
学科: |
|
英文学科: |
|
影响因子: |
|
第一作者所在部门: |
|
英文第一作者所在部门: |
|
论文出处: |
|
英文论文出处: |
|
论文类别: |
|
英文论文类别: |
|
参与作者: |
|
英文参与作者: |
|
|