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Establishing a CRISPR–Cas-like immune system conferring DNA virus resistance in plants
Xiang Ji,Huawei Zhang,Yi Zhang,Yanpeng Wang & Caixia Gao
Nature Plants
Abstract
CRISPR–Cas (clustered, regularly interspaced short palindromic repeats–CRISPR-associated proteins) is an adaptive immune system in many archaea and bacteria that cleaves foreign DNA on the basis of sequence complementarity. Here, using the geminivirus, beet severe curly top virus (BSCTV), transient assays performed in Nicotiana benthamiana demonstrate that the sgRNA–Cas9 constructs inhibit virus accumulation and introduce mutations at the target sequences. Further, transgenic Arabidopsis and N. benthamiana plants overexpressing sgRNA–Cas9 are highly resistant to virus infection.
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论文编号: |
DOI:10.1038/nplants.2015.144 |
论文题目: |
Establishing a CRISPR–Cas-like immune system conferring DNA virus resistance in plants |
英文论文题目: |
Establishing a CRISPR–Cas-like immune system conferring DNA virus resistance in plants |
第一作者: |
Xiang Ji,Huawei Zhang,Yi Zhang,Yanpeng Wang & Caixia Gao |
英文第一作者: |
Xiang Ji,Huawei Zhang,Yi Zhang,Yanpeng Wang & Caixia Gao |
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2015-09-29 |
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CRISPR–Cas (clustered, regularly interspaced short palindromic repeats–CRISPR-associated proteins) is an adaptive immune system in many archaea and bacteria that cleaves foreign DNA on the basis of sequence complementarity. Here, using the geminivirus, beet severe curly top virus (BSCTV), transient assays performed in Nicotiana benthamiana demonstrate that the sgRNA–Cas9 constructs inhibit virus accumulation and introduce mutations at the target sequences. Further, transgenic Arabidopsis and N. benthamiana plants overexpressing sgRNA–Cas9 are highly resistant to virus infection. |
英文摘要: |
CRISPR–Cas (clustered, regularly interspaced short palindromic repeats–CRISPR-associated proteins) is an adaptive immune system in many archaea and bacteria that cleaves foreign DNA on the basis of sequence complementarity. Here, using the geminivirus, beet severe curly top virus (BSCTV), transient assays performed in Nicotiana benthamiana demonstrate that the sgRNA–Cas9 constructs inhibit virus accumulation and introduce mutations at the target sequences. Further, transgenic Arabidopsis and N. benthamiana plants overexpressing sgRNA–Cas9 are highly resistant to virus infection. |
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Nature Plants |
英文刊物名称: |
Nature Plants |
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Xiang Ji,Huawei Zhang,Yi Zhang,Yanpeng Wang & Caixia Gao. Establishing a CRISPR–Cas-like immune system conferring DNA virus resistance in plants. Nature Plants. DOI:10.1038/nplants.2015.144 |
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