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OsDMC1 is not required for homologous pairing in rice meiosis
Hongjun Wang,Qing Hu,Ding Tang,Xiaofei Liu,Guijie Du,Yi Shen,Yafei Li and Zhukuan Cheng
Plant Physiology
Abstract
Meiotic homologous recombination is pivotal to sexual reproduction. DMC1, a conserved recombinase, is involved in directing single end invasion (SEI) between interhomologs during meiotic recombination. In this study, we identified OsDMC1A and OsDMC1B, two closely related proteins in rice with high sequence similarity to DMC1 proteins from other species. Analysis of Osdmc1a and Osdmc1b Tos17 insertion mutants indicated that these genes are functionally redundant. Immunolocalization analysis revealed OsDMC1 foci occurred at leptotene, which disappeared from late pachytene chromosomes in wild-type meiocytes. According to cytological analyses, homologous pairing is accomplished in the Osdmc1a Osdmc1b double mutant, but synapsis is seriously disrupted. The reduced number of bivalents and abnormal OsHEI10 foci in Osdmc1a Osdmc1b establishes an essential role for OsDMC1 in crossover formation. In the absence of OsDMC1, early recombination events probably occur normally, leading to normal localization of γH2AX, PAIR3, OsMRE11, OsCOM1 and OsRAD51C. Moreover, OsDMC1 was not detected in pairing-defective mutants, such as pair2, pair3, Oscom1 and Osrad51c, while it was loaded onto meiotic chromosomes in zep1, Osmer3, Oszip4 and Oshei10. Taken together, these results suggest that during meiosis, OsDMC1 is dispensable for homologous pairing in rice, which is quite different from the DMC1 homologs identified so far in other organisms.
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论文编号: |
DOI:10.1104/pp.16.00167 |
论文题目: |
OsDMC1 is not required for homologous pairing in rice meiosis |
英文论文题目: |
OsDMC1 is not required for homologous pairing in rice meiosis |
第一作者: |
Hongjun Wang,Qing Hu,Ding Tang,Xiaofei Liu,Guijie Du,Yi Shen,Yafei Li and Zhukuan Cheng |
英文第一作者: |
Hongjun Wang,Qing Hu,Ding Tang,Xiaofei Liu,Guijie Du,Yi Shen,Yafei Li and Zhukuan Cheng |
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2016-03-10 |
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Meiotic homologous recombination is pivotal to sexual reproduction. DMC1, a conserved recombinase, is involved in directing single end invasion (SEI) between interhomologs during meiotic recombination. In this study, we identified OsDMC1A and OsDMC1B, two closely related proteins in rice with high sequence similarity to DMC1 proteins from other species. Analysis of Osdmc1a and Osdmc1b Tos17 insertion mutants indicated that these genes are functionally redundant. Immunolocalization analysis revealed OsDMC1 foci occurred at leptotene, which disappeared from late pachytene chromosomes in wild-type meiocytes. According to cytological analyses, homologous pairing is accomplished in the Osdmc1a Osdmc1b double mutant, but synapsis is seriously disrupted. The reduced number of bivalents and abnormal OsHEI10 foci in Osdmc1a Osdmc1b establishes an essential role for OsDMC1 in crossover formation. In the absence of OsDMC1, early recombination events probably occur normally, leading to normal localization of γH2AX, PAIR3, OsMRE11, OsCOM1 and OsRAD51C. Moreover, OsDMC1 was not detected in pairing-defective mutants, such as pair2, pair3, Oscom1 and Osrad51c, while it was loaded onto meiotic chromosomes in zep1, Osmer3, Oszip4 and Oshei10. Taken together, these results suggest that during meiosis, OsDMC1 is dispensable for homologous pairing in rice, which is quite different from the DMC1 homologs identified so far in other organisms. |
英文摘要: |
Meiotic homologous recombination is pivotal to sexual reproduction. DMC1, a conserved recombinase, is involved in directing single end invasion (SEI) between interhomologs during meiotic recombination. In this study, we identified OsDMC1A and OsDMC1B, two closely related proteins in rice with high sequence similarity to DMC1 proteins from other species. Analysis of Osdmc1a and Osdmc1b Tos17 insertion mutants indicated that these genes are functionally redundant. Immunolocalization analysis revealed OsDMC1 foci occurred at leptotene, which disappeared from late pachytene chromosomes in wild-type meiocytes. According to cytological analyses, homologous pairing is accomplished in the Osdmc1a Osdmc1b double mutant, but synapsis is seriously disrupted. The reduced number of bivalents and abnormal OsHEI10 foci in Osdmc1a Osdmc1b establishes an essential role for OsDMC1 in crossover formation. In the absence of OsDMC1, early recombination events probably occur normally, leading to normal localization of γH2AX, PAIR3, OsMRE11, OsCOM1 and OsRAD51C. Moreover, OsDMC1 was not detected in pairing-defective mutants, such as pair2, pair3, Oscom1 and Osrad51c, while it was loaded onto meiotic chromosomes in zep1, Osmer3, Oszip4 and Oshei10. Taken together, these results suggest that during meiosis, OsDMC1 is dispensable for homologous pairing in rice, which is quite different from the DMC1 homologs identified so far in other organisms. |
刊物名称: |
Plant Physiology |
英文刊物名称: |
Plant Physiology |
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其它备注: |
Hongjun Wang,Qing Hu,Ding Tang,Xiaofei Liu,Guijie Du,Yi Shen,Yafei Li and Zhukuan Cheng. OsDMC1 is not required for homologous pairing in rice meiosis. Plant Physiology. DOI:10.1104/pp.16.00167 |
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