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REF6 recognizes a specific DNA sequence to demethylate H3K27me3 and regulate organ boundary formation in Arabidopsis
Xia Cui,Falong Lu,Qi Qiu,Bing Zhou,Lianfeng Gu,Shuaibin Zhang,Yanyuan Kang,Xiekui Cui,Xuan Ma,Qingqing Yao,Jinbiao Ma,Xiaoyu Zhang,Xiaofeng Cao
Nature Genetics
Abstract
RELATIVE OF EARLY FLOWERING 6 (REF6, also known as JMJ12) counteracts Polycomb-mediated gene silencing by removing methyl groups from trimethylated histone H3 lysine 27 (H3K27me3) in hundreds of genes in Arabidopsis thaliana1. Here we show that REF6 function and genome-wide targeting require its four Cys 2His 2 zinc fingers, which directly recognize a CTCTGYTY motif. Motifs bound by REF6 tend to cluster and reside in loci with active chromatin states. Furthermore, REF6 targets CUP-SHAPED COTYLEDON 1 ( CUC1), which harbors CTCTGYTY motifs, to modulate H3K27me3 levels and activate CUC1 expression. Loss of REF6 causes CUC1 repression and defects in cotyledon separation. In contrast, REF6 does not bind CUC2, encoding a close homolog of CUC1, which lacks the CTCTGYTY motif. Collectively, these results identify a new targeting mechanism of an H3K27 demethylase to counteract Polycomb-mediated gene silencing that regulates plant development, including organ boundary formation.
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DOI:10.1038/ng.3556 |
论文题目: |
REF6 recognizes a specific DNA sequence to demethylate H3K27me3 and regulate organ boundary formation in Arabidopsis |
英文论文题目: |
REF6 recognizes a specific DNA sequence to demethylate H3K27me3 and regulate organ boundary formation in Arabidopsis |
第一作者: |
Xia Cui,Falong Lu,Qi Qiu,Bing Zhou,Lianfeng Gu,Shuaibin Zhang,Yanyuan Kang,Xiekui Cui,Xuan Ma,Qingqing Yao,Jinbiao Ma,Xiaoyu Zhang,Xiaofeng Cao |
英文第一作者: |
Xia Cui,Falong Lu,Qi Qiu,Bing Zhou,Lianfeng Gu,Shuaibin Zhang,Yanyuan Kang,Xiekui Cui,Xuan Ma,Qingqing Yao,Jinbiao Ma,Xiaoyu Zhang,Xiaofeng Cao |
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2016-04-26 |
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摘要: |
RELATIVE OF EARLY FLOWERING 6 (REF6, also known as JMJ12) counteracts Polycomb-mediated gene silencing by removing methyl groups from trimethylated histone H3 lysine 27 (H3K27me3) in hundreds of genes in Arabidopsis thaliana1. Here we show that REF6 function and genome-wide targeting require its four Cys 2His 2 zinc fingers, which directly recognize a CTCTGYTY motif. Motifs bound by REF6 tend to cluster and reside in loci with active chromatin states. Furthermore, REF6 targets CUP-SHAPED COTYLEDON 1 ( CUC1), which harbors CTCTGYTY motifs, to modulate H3K27me3 levels and activate CUC1 expression. Loss of REF6 causes CUC1 repression and defects in cotyledon separation. In contrast, REF6 does not bind CUC2, encoding a close homolog of CUC1, which lacks the CTCTGYTY motif. Collectively, these results identify a new targeting mechanism of an H3K27 demethylase to counteract Polycomb-mediated gene silencing that regulates plant development, including organ boundary formation. |
英文摘要: |
RELATIVE OF EARLY FLOWERING 6 (REF6, also known as JMJ12) counteracts Polycomb-mediated gene silencing by removing methyl groups from trimethylated histone H3 lysine 27 (H3K27me3) in hundreds of genes in Arabidopsis thaliana1. Here we show that REF6 function and genome-wide targeting require its four Cys 2His 2 zinc fingers, which directly recognize a CTCTGYTY motif. Motifs bound by REF6 tend to cluster and reside in loci with active chromatin states. Furthermore, REF6 targets CUP-SHAPED COTYLEDON 1 ( CUC1), which harbors CTCTGYTY motifs, to modulate H3K27me3 levels and activate CUC1 expression. Loss of REF6 causes CUC1 repression and defects in cotyledon separation. In contrast, REF6 does not bind CUC2, encoding a close homolog of CUC1, which lacks the CTCTGYTY motif. Collectively, these results identify a new targeting mechanism of an H3K27 demethylase to counteract Polycomb-mediated gene silencing that regulates plant development, including organ boundary formation. |
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Nature Genetics |
英文刊物名称: |
Nature Genetics |
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其它备注: |
Xia Cui,Falong Lu,Qi Qiu,Bing Zhou,Lianfeng Gu,Shuaibin Zhang,Yanyuan Kang,Xiekui Cui,Xuan Ma,Qingqing Yao,Jinbiao Ma,Xiaoyu Zhang,Xiaofeng Cao. REF6 recognizes a specific DNA sequence to demethylate H3K27me3 and regulate organ boundary formation in Arabidopsis. Nature Genetics. DOI:10.1038/ng.3556 |
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