|
FAR-RED ELONGATED HYPOCOTYL3 Activates SEPALLATA2 but Inhibits CLAVATA3 to Regulate Meristem Determinacy and Maintenance in Arabidopsis
Dongming Li, Xing Fu, Lin Guo, Zhigang Huang, Yongpeng Li, Yang Liu, Zishan He, Xiuwei Cao, Xiaohan Ma, Meicheng Zhao, Guohui Zhu, Langtao Xiao, Haiyang Wang, Xuemei Chen, Renyi Liu, and Xigang Liu
Proc Natl Acad Sci U S A
Abstract
Plant meristems are responsible for the generation of all plant tissues and organs. Here we show that the transcription factor (TF) FAR-RED ELONGATED HYPOCOTYL3 (FHY3) plays an important role in both floral meristem (FM) determinacy and shoot apical meristem maintenance in Arabidopsis, in addition to its well-known multifaceted roles in plant growth and development during the vegetative stage. Through genetic analyses, we show that WUSCHEL (WUS) and CLAVATA3 (CLV3), two central players in the establishment and maintenance of meristems, are epistatic to FHY3. Using genome-wide ChIP-seq and RNA-seq data, we identify hundreds of FHY3 target genes in flowers and find that FHY3 mainly acts as a transcriptional repressor in flower development, in contrast to its transcriptional activator role in seedlings. Binding motif-enrichment analyses indicate that FHY3 may coregulate flower development with three flower-specific MADS-domain TFs and four basic helix–loop–helix TFs that are involved in photomorphogenesis. We further demonstrate that CLV3, SEPALLATA1 (SEP1), and SEP2 are FHY3 target genes. In shoot apical meristem, FHY3 directly represses CLV3, which consequently regulates WUS to maintain the stem cell pool. Intriguingly, CLV3 expression did not change significantly in fhy3 and phytochrome B mutants before and after light treatment, indicating that FHY3 and phytochrome B are involved in light-regulated meristem activity. In FM, FHY3 directly represses CLV3, but activates SEP2, to ultimately promote FM determinacy. Taken together, our results reveal insights into the mechanisms of meristem maintenance and determinacy, and illustrate how the roles of a single TF may vary in different organs and developmental stages.
|
论文编号: |
DOI:10.1073/pnas.1602960113 |
论文题目: |
FAR-RED ELONGATED HYPOCOTYL3 Activates SEPALLATA2 but Inhibits CLAVATA3 to Regulate Meristem Determinacy and Maintenance in Arabidopsis |
英文论文题目: |
FAR-RED ELONGATED HYPOCOTYL3 Activates SEPALLATA2 but Inhibits CLAVATA3 to Regulate Meristem Determinacy and Maintenance in Arabidopsis |
第一作者: |
Dongming Li, Xing Fu, Lin Guo, Zhigang Huang, Yongpeng Li, Yang Liu, Zishan He, Xiuwei Cao, Xiaohan Ma, Meicheng Zhao, Guohui Zhu, Langtao Xiao, Haiyang Wang, Xuemei Chen, Renyi Liu, and Xigang Liu |
英文第一作者: |
Dongming Li, Xing Fu, Lin Guo, Zhigang Huang, Yongpeng Li, Yang Liu, Zishan He, Xiuwei Cao, Xiaohan Ma, Meicheng Zhao, Guohui Zhu, Langtao Xiao, Haiyang Wang, Xuemei Chen, Renyi Liu, and Xigang Liu |
联系作者: |
|
英文联系作者: |
|
外单位作者单位: |
|
英文外单位作者单位: |
|
发表年度: |
2016-07-29 |
卷: |
|
期: |
|
页码: |
|
摘要: |
Plant meristems are responsible for the generation of all plant tissues and organs. Here we show that the transcription factor (TF) FAR-RED ELONGATED HYPOCOTYL3 (FHY3) plays an important role in both floral meristem (FM) determinacy and shoot apical meristem maintenance in Arabidopsis, in addition to its well-known multifaceted roles in plant growth and development during the vegetative stage. Through genetic analyses, we show that WUSCHEL (WUS) and CLAVATA3 (CLV3), two central players in the establishment and maintenance of meristems, are epistatic to FHY3. Using genome-wide ChIP-seq and RNA-seq data, we identify hundreds of FHY3 target genes in flowers and find that FHY3 mainly acts as a transcriptional repressor in flower development, in contrast to its transcriptional activator role in seedlings. Binding motif-enrichment analyses indicate that FHY3 may coregulate flower development with three flower-specific MADS-domain TFs and four basic helix–loop–helix TFs that are involved in photomorphogenesis. We further demonstrate that CLV3, SEPALLATA1 (SEP1), and SEP2 are FHY3 target genes. In shoot apical meristem, FHY3 directly represses CLV3, which consequently regulates WUS to maintain the stem cell pool. Intriguingly, CLV3 expression did not change significantly in fhy3 and phytochrome B mutants before and after light treatment, indicating that FHY3 and phytochrome B are involved in light-regulated meristem activity. In FM, FHY3 directly represses CLV3, but activates SEP2, to ultimately promote FM determinacy. Taken together, our results reveal insights into the mechanisms of meristem maintenance and determinacy, and illustrate how the roles of a single TF may vary in different organs and developmental stages. |
英文摘要: |
Plant meristems are responsible for the generation of all plant tissues and organs. Here we show that the transcription factor (TF) FAR-RED ELONGATED HYPOCOTYL3 (FHY3) plays an important role in both floral meristem (FM) determinacy and shoot apical meristem maintenance in Arabidopsis, in addition to its well-known multifaceted roles in plant growth and development during the vegetative stage. Through genetic analyses, we show that WUSCHEL (WUS) and CLAVATA3 (CLV3), two central players in the establishment and maintenance of meristems, are epistatic to FHY3. Using genome-wide ChIP-seq and RNA-seq data, we identify hundreds of FHY3 target genes in flowers and find that FHY3 mainly acts as a transcriptional repressor in flower development, in contrast to its transcriptional activator role in seedlings. Binding motif-enrichment analyses indicate that FHY3 may coregulate flower development with three flower-specific MADS-domain TFs and four basic helix–loop–helix TFs that are involved in photomorphogenesis. We further demonstrate that CLV3, SEPALLATA1 (SEP1), and SEP2 are FHY3 target genes. In shoot apical meristem, FHY3 directly represses CLV3, which consequently regulates WUS to maintain the stem cell pool. Intriguingly, CLV3 expression did not change significantly in fhy3 and phytochrome B mutants before and after light treatment, indicating that FHY3 and phytochrome B are involved in light-regulated meristem activity. In FM, FHY3 directly represses CLV3, but activates SEP2, to ultimately promote FM determinacy. Taken together, our results reveal insights into the mechanisms of meristem maintenance and determinacy, and illustrate how the roles of a single TF may vary in different organs and developmental stages. |
刊物名称: |
Proc Natl Acad Sci U S A |
英文刊物名称: |
Proc Natl Acad Sci U S A |
论文全文: |
|
英文论文全文: |
|
全文链接: |
|
其它备注: |
Dongming Li, Xing Fu, Lin Guo, Zhigang Huang, Yongpeng Li, Yang Liu, Zishan He, Xiuwei Cao, Xiaohan Ma, Meicheng Zhao, Guohui Zhu, Langtao Xiao, Haiyang Wang, Xuemei Chen, Renyi Liu, and Xigang Liu. FAR-RED ELONGATED HYPOCOTYL3 Activates SEPALLATA2 but Inhibits CLAVATA3 to Regulate Meristem Determinacy and Maintenance in Arabidopsis. Proc Natl Acad Sci U S A. DOI:10.1073/pnas.1602960113 |
英文其它备注: |
|
学科: |
|
英文学科: |
|
影响因子: |
|
第一作者所在部门: |
|
英文第一作者所在部门: |
|
论文出处: |
|
英文论文出处: |
|
论文类别: |
|
英文论文类别: |
|
参与作者: |
|
英文参与作者: |
|
|