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Electrostatic Potentials of the S-Locus F-Box Proteins Contribute to the Pollen S Specificity in Self-Incompatibility in Petunia hybrida
Junhui Li, Yue Zhang, Yanzhai Song, Hui Zhang, Jiangbo Fan, Qun Li, Dongfen Zhang, Yongbiao Xue
Plant Journal
Abstract
Self-incompatibility (SI) is a self/non-self discrimination system widely found in angiosperms and, in many species, is controlled by a single polymorphic S-locus. In the Solanaceae, Rosaceae and Plantaginaceae, the S-locus encodes a single S-RNase and a cluster of S-locus F-box (SLF) proteins to control the pistil and pollen expression of SI, respectively. Previous studies have shown that their cytosolic interactions determine their recognition specificity, but the physical force between their interactions remains unclear. In this study, we show that the electrostatic potentials of SLF contribute to the pollen S specificity through a physical mechanism of “like charges repel and unlike charges attract” between SLFs and S-RNases in Petunia hybrida. Strikingly, alteration of a single C-terminal amino acid of SLF reversed its surface electrostatic potentials and subsequently the pollen S specificity. Collectively, our results reveal that the electrostatic potentials act as a major physical force between cytosolic SLFs and S-RNases, providing a mechanistic insight into the self/non-self discrimination between cytosolic proteins in angiosperms.
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论文编号: |
DOI:10.1111/tpj.13318 |
论文题目: |
Electrostatic Potentials of the S-Locus F-Box Proteins Contribute to the Pollen S Specificity in Self-Incompatibility in Petunia hybrida |
英文论文题目: |
Electrostatic Potentials of the S-Locus F-Box Proteins Contribute to the Pollen S Specificity in Self-Incompatibility in Petunia hybrida |
第一作者: |
Junhui Li, Yue Zhang, Yanzhai Song, Hui Zhang, Jiangbo Fan, Qun Li, Dongfen Zhang, Yongbiao Xue |
英文第一作者: |
Junhui Li, Yue Zhang, Yanzhai Song, Hui Zhang, Jiangbo Fan, Qun Li, Dongfen Zhang, Yongbiao Xue |
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2016-08-30 |
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摘要: |
Self-incompatibility (SI) is a self/non-self discrimination system widely found in angiosperms and, in many species, is controlled by a single polymorphic S-locus. In the Solanaceae, Rosaceae and Plantaginaceae, the S-locus encodes a single S-RNase and a cluster of S-locus F-box (SLF) proteins to control the pistil and pollen expression of SI, respectively. Previous studies have shown that their cytosolic interactions determine their recognition specificity, but the physical force between their interactions remains unclear. In this study, we show that the electrostatic potentials of SLF contribute to the pollen S specificity through a physical mechanism of “like charges repel and unlike charges attract” between SLFs and S-RNases in Petunia hybrida. Strikingly, alteration of a single C-terminal amino acid of SLF reversed its surface electrostatic potentials and subsequently the pollen S specificity. Collectively, our results reveal that the electrostatic potentials act as a major physical force between cytosolic SLFs and S-RNases, providing a mechanistic insight into the self/non-self discrimination between cytosolic proteins in angiosperms. |
英文摘要: |
Self-incompatibility (SI) is a self/non-self discrimination system widely found in angiosperms and, in many species, is controlled by a single polymorphic S-locus. In the Solanaceae, Rosaceae and Plantaginaceae, the S-locus encodes a single S-RNase and a cluster of S-locus F-box (SLF) proteins to control the pistil and pollen expression of SI, respectively. Previous studies have shown that their cytosolic interactions determine their recognition specificity, but the physical force between their interactions remains unclear. In this study, we show that the electrostatic potentials of SLF contribute to the pollen S specificity through a physical mechanism of “like charges repel and unlike charges attract” between SLFs and S-RNases in Petunia hybrida. Strikingly, alteration of a single C-terminal amino acid of SLF reversed its surface electrostatic potentials and subsequently the pollen S specificity. Collectively, our results reveal that the electrostatic potentials act as a major physical force between cytosolic SLFs and S-RNases, providing a mechanistic insight into the self/non-self discrimination between cytosolic proteins in angiosperms. |
刊物名称: |
Plant Journal |
英文刊物名称: |
Plant Journal |
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其它备注: |
Junhui Li, Yue Zhang, Yanzhai Song, Hui Zhang, Jiangbo Fan, Qun Li, Dongfen Zhang, Yongbiao Xue. Electrostatic Potentials of the S-Locus F-Box Proteins Contribute to the Pollen S Specificity in Self-Incompatibility in Petunia hybrida. Plant Journal. DOI:10.1111/tpj.13318 |
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