|
Quantitative Profiling of Spreading-Coupled Protein Tyrosine Phosphorylation in Migratory Cells
Yajun Xie, Jinlong Wang, Yuanya Zhang, Xiaofei Liu, Xiaorong Wang, Kehui Liu, Xiahe Huang, and Yingchun Wang
Scientific Reports
Abstract
Protein tyrosine phosphorylation is an important mechanism that regulates cytoskeleton reorganization and cell spreading of migratory cells. A number of cytoskeletal proteins are known to be tyrosine phosphorylated (pY) in different cellular processes. However, the profile of pY proteins during different stages of cell spreading has not been available. Using immunoafffinity enrichment of pY proteins coupled with label free quantitative proteomics, we quantitatively identified 447 pY proteins in the migratory ECV-304 cells at the early spreading (adhesion) and the active spreading stages. We found that pY levels of the majority of the quantified proteins were significantly increased in the active spreading stage compared with the early spreading stage, suggesting that active cell spreading is concomitant with extra tyrosine phosphorylation. The major categories of proteins impacted by tyrosine phosphorylation are involved in cytoskeleton and focal adhesion regulation, protein translation and degradation. Our findings, for the first time, dissect the cell spreading-specific pY signals from the adhesion induced pY signals, and provide a valuable resource for the future mechanistic research regarding the regulation of cell spreading.
|
论文编号: |
DOI:10.1038/srep31811 |
论文题目: |
Quantitative Profiling of Spreading-Coupled Protein Tyrosine Phosphorylation in Migratory Cells |
英文论文题目: |
Quantitative Profiling of Spreading-Coupled Protein Tyrosine Phosphorylation in Migratory Cells |
第一作者: |
Yajun Xie, Jinlong Wang, Yuanya Zhang, Xiaofei Liu, Xiaorong Wang, Kehui Liu, Xiahe Huang, and Yingchun Wang |
英文第一作者: |
Yajun Xie, Jinlong Wang, Yuanya Zhang, Xiaofei Liu, Xiaorong Wang, Kehui Liu, Xiahe Huang, and Yingchun Wang |
联系作者: |
|
英文联系作者: |
|
外单位作者单位: |
|
英文外单位作者单位: |
|
发表年度: |
2016-09-02 |
卷: |
|
期: |
|
页码: |
|
摘要: |
Protein tyrosine phosphorylation is an important mechanism that regulates cytoskeleton reorganization and cell spreading of migratory cells. A number of cytoskeletal proteins are known to be tyrosine phosphorylated (pY) in different cellular processes. However, the profile of pY proteins during different stages of cell spreading has not been available. Using immunoafffinity enrichment of pY proteins coupled with label free quantitative proteomics, we quantitatively identified 447 pY proteins in the migratory ECV-304 cells at the early spreading (adhesion) and the active spreading stages. We found that pY levels of the majority of the quantified proteins were significantly increased in the active spreading stage compared with the early spreading stage, suggesting that active cell spreading is concomitant with extra tyrosine phosphorylation. The major categories of proteins impacted by tyrosine phosphorylation are involved in cytoskeleton and focal adhesion regulation, protein translation and degradation. Our findings, for the first time, dissect the cell spreading-specific pY signals from the adhesion induced pY signals, and provide a valuable resource for the future mechanistic research regarding the regulation of cell spreading. |
英文摘要: |
Protein tyrosine phosphorylation is an important mechanism that regulates cytoskeleton reorganization and cell spreading of migratory cells. A number of cytoskeletal proteins are known to be tyrosine phosphorylated (pY) in different cellular processes. However, the profile of pY proteins during different stages of cell spreading has not been available. Using immunoafffinity enrichment of pY proteins coupled with label free quantitative proteomics, we quantitatively identified 447 pY proteins in the migratory ECV-304 cells at the early spreading (adhesion) and the active spreading stages. We found that pY levels of the majority of the quantified proteins were significantly increased in the active spreading stage compared with the early spreading stage, suggesting that active cell spreading is concomitant with extra tyrosine phosphorylation. The major categories of proteins impacted by tyrosine phosphorylation are involved in cytoskeleton and focal adhesion regulation, protein translation and degradation. Our findings, for the first time, dissect the cell spreading-specific pY signals from the adhesion induced pY signals, and provide a valuable resource for the future mechanistic research regarding the regulation of cell spreading. |
刊物名称: |
Scientific Reports |
英文刊物名称: |
Scientific Reports |
论文全文: |
|
英文论文全文: |
|
全文链接: |
|
其它备注: |
Yajun Xie, Jinlong Wang, Yuanya Zhang, Xiaofei Liu, Xiaorong Wang, Kehui Liu, Xiahe Huang, and Yingchun Wang. Quantitative Profiling of Spreading-Coupled Protein Tyrosine Phosphorylation in Migratory Cells. Scientific Reports. DOI: 10.1038/srep31811 |
英文其它备注: |
|
学科: |
|
英文学科: |
|
影响因子: |
|
第一作者所在部门: |
|
英文第一作者所在部门: |
|
论文出处: |
|
英文论文出处: |
|
论文类别: |
|
英文论文类别: |
|
参与作者: |
|
英文参与作者: |
|
|