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P31comet, a Member of the Synaptonemal Complex, Participates in Meiotic DSB Formation in Rice
Jianhui Ji, Ding Tang, Yi Shen, Zhihui Xue, Hongjun Wang, Wenqing Shi, Chao Zhang, Guijie Du, Yafei Li, and Zhukuan Cheng
Proc Natl Acad Sci U S A
Abstract
The human mitotic arrest-deficient 2 (Mad2) binding protein p31comet participates in the spindle checkpoint and coordinates cell cycle events in mitosis although its function in meiosis remains unknown in all organisms. Here, we reveal P31comet as a synaptonemal complex (SC) protein in rice (Oryza sativa L.). In p31comet, homologous pairing and synapsis are eliminated, leading to the homologous nondisjunction and complete sterility. The failure in loading of histone H2AX phosphorylation (γH2AX) in p31comet, together with the suppressed chromosome fragmentation in rice completion of meiotic recombination 1 (com1) p31comet and radiation sensitive 51c (rad51c) p31comet double mutants, indicates that P31comet plays an essential role in double-strand break (DSB) formation. Interestingly, the dynamic colocalization pattern between P31comet and ZEP1 (a transverse filament protein of SC) by immunostaining, as well as the interaction between P31comet and CENTRAL REGION COMPONENT 1 (CRC1) in yeast two-hybrid assays, suggests possible involvement of P31comet in SC installation. Together, these data indicate that P31comet plays a key role in DSB formation and SC installation, mainly through its cooperation with CRC1.
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论文编号: |
DOI:10.1073/pnas.1607334113 |
论文题目: |
P31comet, a Member of the Synaptonemal Complex, Participates in Meiotic DSB Formation in Rice |
英文论文题目: |
P31comet, a Member of the Synaptonemal Complex, Participates in Meiotic DSB Formation in Rice |
第一作者: |
Jianhui Ji, Ding Tang, Yi Shen, Zhihui Xue, Hongjun Wang, Wenqing Shi, Chao Zhang, Guijie Du, Yafei Li, and Zhukuan Cheng |
英文第一作者: |
Jianhui Ji, Ding Tang, Yi Shen, Zhihui Xue, Hongjun Wang, Wenqing Shi, Chao Zhang, Guijie Du, Yafei Li, and Zhukuan Cheng |
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2016-09-07 |
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The human mitotic arrest-deficient 2 (Mad2) binding protein p31comet participates in the spindle checkpoint and coordinates cell cycle events in mitosis although its function in meiosis remains unknown in all organisms. Here, we reveal P31comet as a synaptonemal complex (SC) protein in rice (Oryza sativa L.). In p31comet, homologous pairing and synapsis are eliminated, leading to the homologous nondisjunction and complete sterility. The failure in loading of histone H2AX phosphorylation (γH2AX) in p31comet, together with the suppressed chromosome fragmentation in rice completion of meiotic recombination 1 (com1) p31comet and radiation sensitive 51c (rad51c) p31comet double mutants, indicates that P31comet plays an essential role in double-strand break (DSB) formation. Interestingly, the dynamic colocalization pattern between P31comet and ZEP1 (a transverse filament protein of SC) by immunostaining, as well as the interaction between P31comet and CENTRAL REGION COMPONENT 1 (CRC1) in yeast two-hybrid assays, suggests possible involvement of P31comet in SC installation. Together, these data indicate that P31comet plays a key role in DSB formation and SC installation, mainly through its cooperation with CRC1. |
英文摘要: |
The human mitotic arrest-deficient 2 (Mad2) binding protein p31comet participates in the spindle checkpoint and coordinates cell cycle events in mitosis although its function in meiosis remains unknown in all organisms. Here, we reveal P31comet as a synaptonemal complex (SC) protein in rice (Oryza sativa L.). In p31comet, homologous pairing and synapsis are eliminated, leading to the homologous nondisjunction and complete sterility. The failure in loading of histone H2AX phosphorylation (γH2AX) in p31comet, together with the suppressed chromosome fragmentation in rice completion of meiotic recombination 1 (com1) p31comet and radiation sensitive 51c (rad51c) p31comet double mutants, indicates that P31comet plays an essential role in double-strand break (DSB) formation. Interestingly, the dynamic colocalization pattern between P31comet and ZEP1 (a transverse filament protein of SC) by immunostaining, as well as the interaction between P31comet and CENTRAL REGION COMPONENT 1 (CRC1) in yeast two-hybrid assays, suggests possible involvement of P31comet in SC installation. Together, these data indicate that P31comet plays a key role in DSB formation and SC installation, mainly through its cooperation with CRC1. |
刊物名称: |
Proc Natl Acad Sci U S A |
英文刊物名称: |
Proc Natl Acad Sci U S A |
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其它备注: |
Jianhui Ji, Ding Tang, Yi Shen, Zhihui Xue, Hongjun Wang, Wenqing Shi, Chao Zhang, Guijie Du, Yafei Li, and Zhukuan Cheng. P31comet, a Member of the Synaptonemal Complex, Participates in Meiotic DSB Formation in Rice. Proc Natl Acad Sci U S A. DOI:10.1073/pnas.1607334113 |
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