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Mitogen-Activated Protein Kinase Cascade MKK7-MPK6 Plays an Important Role in Plant Development and Regulates Shoot Branching by Phosphorylating PIN1 in Arabidopsis
Weiyan Jia, Baohua Li, Shujia Li, Yan Liang, Xiaowei Wu, Mei Ma, Jiyao Wang, Jin Gao, Yueyue Cai, Yuanya Zhang, Yingchun Wang, Jiayang Li, and Yonghong Wang
PLOS Biology
Abstract
Emerging evidences exhibit that mitogen-activated protein kinase (MAPK or MPK) signaling pathways are connected with many aspects of plant development. The complexity of MAPK cascades raises challenges not only to identify the MAPK module in planta but also to define the specific role of an individual module. So far, our knowledge of MAPK signaling has been largely restricted to a small subset of MAPK cascades. Our previous study has characterized an Arabidopsis bushy and dwarf1 ( bud1) mutant, in which the MAP Kinase Kinase 7 (MKK7) was constitutively activated, resulting in multiple phenotypic alterations. In this study, we found that MPK3 and MPK6 are the substrates for phosphorylation by MKK7 in planta. Genetic analysis showed that MKK7-MPK6 cascade is specifically responsible for the regulation of shoot branching, hypocotyl gravitropism, filament elongation, and lateral root formation, while MKK7-MPK3 cascade is mainly involved in leaf morphology. We further demonstrated that the MKK7-MPK6 cascade controls shoot branching by phosphorylating Ser337 on PIN1, which affects the basal localization of PIN1 in xylem parenchyma cells and polar auxin transport in the primary stem. Our results not only specify the functions of the MKK7-MPK6 cascade but also reveal a novel mechanism for PIN1 phosphorylation, establishing a molecular link between the MAPK cascade and auxin-regulated plant development.
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DOI:10.1371/journal.pbio.1002550 |
论文题目: |
Mitogen-Activated Protein Kinase Cascade MKK7-MPK6 Plays an Important Role in Plant Development and Regulates Shoot Branching by Phosphorylating PIN1 in Arabidopsis |
英文论文题目: |
Mitogen-Activated Protein Kinase Cascade MKK7-MPK6 Plays an Important Role in Plant Development and Regulates Shoot Branching by Phosphorylating PIN1 in Arabidopsis |
第一作者: |
Weiyan Jia, Baohua Li, Shujia Li, Yan Liang, Xiaowei Wu, Mei Ma, Jiyao Wang, Jin Gao, Yueyue Cai, Yuanya Zhang, Yingchun Wang, Jiayang Li, and Yonghong Wang |
英文第一作者: |
Weiyan Jia, Baohua Li, Shujia Li, Yan Liang, Xiaowei Wu, Mei Ma, Jiyao Wang, Jin Gao, Yueyue Cai, Yuanya Zhang, Yingchun Wang, Jiayang Li, and Yonghong Wang |
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2016-09-13 |
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Emerging evidences exhibit that mitogen-activated protein kinase (MAPK or MPK) signaling pathways are connected with many aspects of plant development. The complexity of MAPK cascades raises challenges not only to identify the MAPK module in planta but also to define the specific role of an individual module. So far, our knowledge of MAPK signaling has been largely restricted to a small subset of MAPK cascades. Our previous study has characterized an Arabidopsis bushy and dwarf1 ( bud1) mutant, in which the MAP Kinase Kinase 7 (MKK7) was constitutively activated, resulting in multiple phenotypic alterations. In this study, we found that MPK3 and MPK6 are the substrates for phosphorylation by MKK7 in planta. Genetic analysis showed that MKK7-MPK6 cascade is specifically responsible for the regulation of shoot branching, hypocotyl gravitropism, filament elongation, and lateral root formation, while MKK7-MPK3 cascade is mainly involved in leaf morphology. We further demonstrated that the MKK7-MPK6 cascade controls shoot branching by phosphorylating Ser337 on PIN1, which affects the basal localization of PIN1 in xylem parenchyma cells and polar auxin transport in the primary stem. Our results not only specify the functions of the MKK7-MPK6 cascade but also reveal a novel mechanism for PIN1 phosphorylation, establishing a molecular link between the MAPK cascade and auxin-regulated plant development. |
英文摘要: |
Emerging evidences exhibit that mitogen-activated protein kinase (MAPK or MPK) signaling pathways are connected with many aspects of plant development. The complexity of MAPK cascades raises challenges not only to identify the MAPK module in planta but also to define the specific role of an individual module. So far, our knowledge of MAPK signaling has been largely restricted to a small subset of MAPK cascades. Our previous study has characterized an Arabidopsis bushy and dwarf1 ( bud1) mutant, in which the MAP Kinase Kinase 7 (MKK7) was constitutively activated, resulting in multiple phenotypic alterations. In this study, we found that MPK3 and MPK6 are the substrates for phosphorylation by MKK7 in planta. Genetic analysis showed that MKK7-MPK6 cascade is specifically responsible for the regulation of shoot branching, hypocotyl gravitropism, filament elongation, and lateral root formation, while MKK7-MPK3 cascade is mainly involved in leaf morphology. We further demonstrated that the MKK7-MPK6 cascade controls shoot branching by phosphorylating Ser337 on PIN1, which affects the basal localization of PIN1 in xylem parenchyma cells and polar auxin transport in the primary stem. Our results not only specify the functions of the MKK7-MPK6 cascade but also reveal a novel mechanism for PIN1 phosphorylation, establishing a molecular link between the MAPK cascade and auxin-regulated plant development. |
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PLOS Biology |
英文刊物名称: |
PLOS Biology |
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其它备注: |
Weiyan Jia, Baohua Li, Shujia Li, Yan Liang, Xiaowei Wu, Mei Ma, Jiyao Wang, Jin Gao, Yueyue Cai, Yuanya Zhang, Yingchun Wang, Jiayang Li, and Yonghong Wang. Mitogen-Activated Protein Kinase Cascade MKK7-MPK6 Plays an Important Role in Plant Development and Regulates Shoot Branching by Phosphorylating PIN1 in Arabidopsis. PLOS Biology. DOI:10.1371/journal.pbio.1002550 |
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