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Rice Ferredoxin-Dependent Glutamate Synthase Regulates Nitrogen-Carbon Metabolomes and Is Genetically Differentiated between japonica and indica Subspecies
Xiaolu Yang, Jinqiang Nian, Qingjun Xie, Jian Feng, Fengxia Zhang, Hongwei Jing, Jian Zhang, Guojun Dong, Yan Liang, Juli Peng, Guodong Wang, Qian Qian, Jianru Zuo
Molecular Plant
Abstract
Plants assimilate inorganic nitrogen absorbed from soil into organic forms as Gln and Glu through the glutamine synthetase/glutamine:2-oxoglutarate amidotransferase (GS/GOGAT) cycle. Whereas GS catalyzes the formation of Gln from Glu and ammonia, GOGAT catalyzes the transfer of an amide group from Gln to 2-oxoglutarate to produce two molecules of Glu. However, the regulatory role of the GS/GOGAT cycle in the carbon-nitrogen balance is not well understood. Here, we report the functional characterization of the rice ABNORMAL CYTOKININ RESPONSE 1 (ABC1) gene that encodes an Fd-GOGAT. Whereas the weak mutant allele abc1-1 mutant shows a typical nitrogen-deficient syndrome, the T-DNA insertional mutant abc1-2 is seedling lethal. Metabolomics analysis revealed the accumulation of excessive amount of amino acids with high N/C ratio (Gln and Asn) and several intermediates in the tricarboxylic acid cycle in abc1-1, suggesting that ABC1 plays a critical role in regulating nitrogen assimilation and carbon-nitrogen balance. Five non-synonymous single nucleotide polymorphisms were identified in the ABC1 coding region and characterized as 3 distinct haplotypes, which have been highly and specifically differentiated between japonica and indicasubspecies. These results suggest that ABC1/OsFd-GOGAT is essential for plant growth and development by modulating nitrogen assimilation and the carbon-nitrogen balance.
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DOI:10.1016/j.molp.2016.09.004 |
论文题目: |
Rice Ferredoxin-Dependent Glutamate Synthase Regulates Nitrogen-Carbon Metabolomes and Is Genetically Differentiated between japonica and indica Subspecies |
英文论文题目: |
Rice Ferredoxin-Dependent Glutamate Synthase Regulates Nitrogen-Carbon Metabolomes and Is Genetically Differentiated between japonica and indica Subspecies |
第一作者: |
Xiaolu Yang, Jinqiang Nian, Qingjun Xie, Jian Feng, Fengxia Zhang, Hongwei Jing, Jian Zhang, Guojun Dong, Yan Liang, Juli Peng, Guodong Wang, Qian Qian, Jianru Zuo |
英文第一作者: |
Xiaolu Yang, Jinqiang Nian, Qingjun Xie, Jian Feng, Fengxia Zhang, Hongwei Jing, Jian Zhang, Guojun Dong, Yan Liang, Juli Peng, Guodong Wang, Qian Qian, Jianru Zuo |
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2016-09-26 |
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摘要: |
Plants assimilate inorganic nitrogen absorbed from soil into organic forms as Gln and Glu through the glutamine synthetase/glutamine:2-oxoglutarate amidotransferase (GS/GOGAT) cycle. Whereas GS catalyzes the formation of Gln from Glu and ammonia, GOGAT catalyzes the transfer of an amide group from Gln to 2-oxoglutarate to produce two molecules of Glu. However, the regulatory role of the GS/GOGAT cycle in the carbon-nitrogen balance is not well understood. Here, we report the functional characterization of the rice ABNORMAL CYTOKININ RESPONSE 1 (ABC1) gene that encodes an Fd-GOGAT. Whereas the weak mutant allele abc1-1 mutant shows a typical nitrogen-deficient syndrome, the T-DNA insertional mutant abc1-2 is seedling lethal. Metabolomics analysis revealed the accumulation of excessive amount of amino acids with high N/C ratio (Gln and Asn) and several intermediates in the tricarboxylic acid cycle in abc1-1, suggesting that ABC1 plays a critical role in regulating nitrogen assimilation and carbon-nitrogen balance. Five non-synonymous single nucleotide polymorphisms were identified in the ABC1 coding region and characterized as 3 distinct haplotypes, which have been highly and specifically differentiated between japonica and indicasubspecies. These results suggest that ABC1/OsFd-GOGAT is essential for plant growth and development by modulating nitrogen assimilation and the carbon-nitrogen balance. |
英文摘要: |
Plants assimilate inorganic nitrogen absorbed from soil into organic forms as Gln and Glu through the glutamine synthetase/glutamine:2-oxoglutarate amidotransferase (GS/GOGAT) cycle. Whereas GS catalyzes the formation of Gln from Glu and ammonia, GOGAT catalyzes the transfer of an amide group from Gln to 2-oxoglutarate to produce two molecules of Glu. However, the regulatory role of the GS/GOGAT cycle in the carbon-nitrogen balance is not well understood. Here, we report the functional characterization of the rice ABNORMAL CYTOKININ RESPONSE 1 (ABC1) gene that encodes an Fd-GOGAT. Whereas the weak mutant allele abc1-1 mutant shows a typical nitrogen-deficient syndrome, the T-DNA insertional mutant abc1-2 is seedling lethal. Metabolomics analysis revealed the accumulation of excessive amount of amino acids with high N/C ratio (Gln and Asn) and several intermediates in the tricarboxylic acid cycle in abc1-1, suggesting that ABC1 plays a critical role in regulating nitrogen assimilation and carbon-nitrogen balance. Five non-synonymous single nucleotide polymorphisms were identified in the ABC1 coding region and characterized as 3 distinct haplotypes, which have been highly and specifically differentiated between japonica and indicasubspecies. These results suggest that ABC1/OsFd-GOGAT is essential for plant growth and development by modulating nitrogen assimilation and the carbon-nitrogen balance. |
刊物名称: |
Molecular Plant |
英文刊物名称: |
Molecular Plant |
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其它备注: |
Xiaolu Yang, Jinqiang Nian, Qingjun Xie, Jian Feng, Fengxia Zhang, Hongwei Jing, Jian Zhang, Guojun Dong, Yan Liang, Juli Peng, Guodong Wang, Qian Qian, Jianru Zuo. Rice Ferredoxin-Dependent Glutamate Synthase Regulates Nitrogen-Carbon Metabolomes and Is Genetically Differentiated between japonica and indica Subspecies. Molecular Plant. DOI:10.1016/j.molp.2016.09.004 |
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