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Functional Conservation and Divergence of GmCHLI Genes in Polyploid Soybean.
Qing Li, Chao Fang, Zongbiao Duan, Yucheng Liu, Hao Qin, Jixiang Zhang, Peng Sun, Wenbin Li,
Guodong Wang and Zhixi Tian
Plant Journal
Abstract
Polyploidy is prevalent in nature. As the fate of duplicated genes becomes more complicated when the encoded proteins function as oligomers, functional investigations into duplicated oligomer-encoding genes in polyploid genomes will facilitate our understanding of how traits are expressed. In this study, we identified GmCHLI1, a gene encoding the I subunit of magnesium (Mg)-chelatase, which functions in hexamers as responsible for the semi-dominant etiolation phenotype in soybean. Four GmCHLI copies derived from two polyploidy events were identified in the soybean genome. Further investigation with regard to expression patterns indicated that these four copies have diverged into two pairs; mutation in the other copy of the pair that includes GmCHLI1 also resulted in a chlorophyll-deficient phenotype. Protein interaction assays showed that these four GmCHLIs can interact with each other, but stronger interactions were found with mutated subunits. The results indicate that, in polyploidy, deficiency in each copy of duplicated oligomer-encoding genes could result in a mutant phenotype due to hetero-oligomer formation, which is different from the model of allelic dosage or functional redundancy. In addition, we interestingly found an increase in isoflavonoids in the heterozygous etiolated plants, which might be useful for improving soybean seed quality.
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论文编号: |
DOI:10.1111/tpj.13282 |
论文题目: |
Functional Conservation and Divergence of GmCHLI Genes in Polyploid Soybean. |
英文论文题目: |
Functional Conservation and Divergence of GmCHLI Genes in Polyploid Soybean. |
第一作者: |
Qing Li, Chao Fang, Zongbiao Duan, Yucheng Liu, Hao Qin, Jixiang Zhang, Peng Sun, Wenbin Li,
Guodong Wang and Zhixi Tian |
英文第一作者: |
Qing Li, Chao Fang, Zongbiao Duan, Yucheng Liu, Hao Qin, Jixiang Zhang, Peng Sun, Wenbin Li,
Guodong Wang and Zhixi Tian |
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2016-11-11 |
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摘要: |
Polyploidy is prevalent in nature. As the fate of duplicated genes becomes more complicated when the encoded proteins function as oligomers, functional investigations into duplicated oligomer-encoding genes in polyploid genomes will facilitate our understanding of how traits are expressed. In this study, we identified GmCHLI1, a gene encoding the I subunit of magnesium (Mg)-chelatase, which functions in hexamers as responsible for the semi-dominant etiolation phenotype in soybean. Four GmCHLI copies derived from two polyploidy events were identified in the soybean genome. Further investigation with regard to expression patterns indicated that these four copies have diverged into two pairs; mutation in the other copy of the pair that includes GmCHLI1 also resulted in a chlorophyll-deficient phenotype. Protein interaction assays showed that these four GmCHLIs can interact with each other, but stronger interactions were found with mutated subunits. The results indicate that, in polyploidy, deficiency in each copy of duplicated oligomer-encoding genes could result in a mutant phenotype due to hetero-oligomer formation, which is different from the model of allelic dosage or functional redundancy. In addition, we interestingly found an increase in isoflavonoids in the heterozygous etiolated plants, which might be useful for improving soybean seed quality. |
英文摘要: |
Polyploidy is prevalent in nature. As the fate of duplicated genes becomes more complicated when the encoded proteins function as oligomers, functional investigations into duplicated oligomer-encoding genes in polyploid genomes will facilitate our understanding of how traits are expressed. In this study, we identified GmCHLI1, a gene encoding the I subunit of magnesium (Mg)-chelatase, which functions in hexamers as responsible for the semi-dominant etiolation phenotype in soybean. Four GmCHLI copies derived from two polyploidy events were identified in the soybean genome. Further investigation with regard to expression patterns indicated that these four copies have diverged into two pairs; mutation in the other copy of the pair that includes GmCHLI1 also resulted in a chlorophyll-deficient phenotype. Protein interaction assays showed that these four GmCHLIs can interact with each other, but stronger interactions were found with mutated subunits. The results indicate that, in polyploidy, deficiency in each copy of duplicated oligomer-encoding genes could result in a mutant phenotype due to hetero-oligomer formation, which is different from the model of allelic dosage or functional redundancy. In addition, we interestingly found an increase in isoflavonoids in the heterozygous etiolated plants, which might be useful for improving soybean seed quality. |
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Plant Journal |
英文刊物名称: |
Plant Journal |
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其它备注: |
Qing Li, Chao Fang, Zongbiao Duan, Yucheng Liu, Hao Qin, Jixiang Zhang, Peng Sun, Wenbin Li, Guodong Wang and Zhixi Tian. Functional Conservation and Divergence of GmCHLI Genes in Polyploid Soybean. Plant Journal. DOI: 10.1111/tpj.13282 |
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