|
Two Trichome Birefringence-like proteins Mediate Xylan Acetylation, Which Is Essential for Leaf Blight Resistance in Rice
Yaping Gao, Congwu He, Dongmei Zhang, Xiangling Liu, Zuopeng Xu, Tian Yanbao, Xue-Hui Liu, Shanshan Zang, Markus Pauly, Yihua Zhou and Baocai Zhang
Plant Physiology
Abstract
Acetylation is a ubiquitous modification on cell wall polymers, which play a structural role in plant growth and stress defenses. However, the mechanisms for how crop plants accomplish cell-wall polymer O-acetylation are largely unknown. Here, we report on the isolation and characterization of two trichome birefringence-like (tbl) mutants in rice, which are affected in xylan O-acetylation. ostbl1 and ostbl2 single mutant and the tbl1 tbl2 double mutant displayed a stunted growth phenotype with varied degree of dwarfism. As shown by chemical assays, the wall acetylation level is affected in the mutants and the knock-down and overexpression transgenic plants. Furthermore, nuclear magnetic resonance (NMR) spectroscopy analyses showed that all those mutants have varied decreases in xylan monoacetylation. The divergent expression level of OsTBL1 and OsTBL2 explained the chemotype difference and indicated that OsTBL1 is a functionally dominant gene. OsTBL1 was found to be Golgi-localized. The recombinant OsTBL1 protein incorporates acetyl groups onto xylan. By using xylopentaose (X5), a preferring acceptor substrate, OsTBL1 can transfer up to four acetyl residues onto X5 and this activity showed saturable kinetics. 2D-NMR spectroscopy specified that OsTBL1 transfers acetate to both 2-O and 3-O site of xylosyl residues. In addition, ostbl1 and tbl1 tbl2 displayed susceptibility to rice blight disease, indicating that this xylan modification is required for pathogen resistance. This study offers us the insights into the mechanisms and functions of xylan acetylation in crop plants.
|
论文编号: |
DOI:10.1104/pp.16.01618 |
论文题目: |
Two Trichome Birefringence-like proteins Mediate Xylan Acetylation, Which Is Essential for Leaf Blight Resistance in Rice |
英文论文题目: |
Two Trichome Birefringence-like proteins Mediate Xylan Acetylation, Which Is Essential for Leaf Blight Resistance in Rice |
第一作者: |
Yaping Gao, Congwu He, Dongmei Zhang, Xiangling Liu, Zuopeng Xu, Tian Yanbao, Xue-Hui Liu, Shanshan Zang, Markus Pauly, Yihua Zhou and Baocai Zhang |
英文第一作者: |
Yaping Gao, Congwu He, Dongmei Zhang, Xiangling Liu, Zuopeng Xu, Tian Yanbao, Xue-Hui Liu, Shanshan Zang, Markus Pauly, Yihua Zhou and Baocai Zhang |
联系作者: |
|
英文联系作者: |
|
外单位作者单位: |
|
英文外单位作者单位: |
|
发表年度: |
2016-11-19 |
卷: |
|
期: |
|
页码: |
|
摘要: |
Acetylation is a ubiquitous modification on cell wall polymers, which play a structural role in plant growth and stress defenses. However, the mechanisms for how crop plants accomplish cell-wall polymer O-acetylation are largely unknown. Here, we report on the isolation and characterization of two trichome birefringence-like (tbl) mutants in rice, which are affected in xylan O-acetylation. ostbl1 and ostbl2 single mutant and the tbl1 tbl2 double mutant displayed a stunted growth phenotype with varied degree of dwarfism. As shown by chemical assays, the wall acetylation level is affected in the mutants and the knock-down and overexpression transgenic plants. Furthermore, nuclear magnetic resonance (NMR) spectroscopy analyses showed that all those mutants have varied decreases in xylan monoacetylation. The divergent expression level of OsTBL1 and OsTBL2 explained the chemotype difference and indicated that OsTBL1 is a functionally dominant gene. OsTBL1 was found to be Golgi-localized. The recombinant OsTBL1 protein incorporates acetyl groups onto xylan. By using xylopentaose (X5), a preferring acceptor substrate, OsTBL1 can transfer up to four acetyl residues onto X5 and this activity showed saturable kinetics. 2D-NMR spectroscopy specified that OsTBL1 transfers acetate to both 2-O and 3-O site of xylosyl residues. In addition, ostbl1 and tbl1 tbl2 displayed susceptibility to rice blight disease, indicating that this xylan modification is required for pathogen resistance. This study offers us the insights into the mechanisms and functions of xylan acetylation in crop plants. |
英文摘要: |
Acetylation is a ubiquitous modification on cell wall polymers, which play a structural role in plant growth and stress defenses. However, the mechanisms for how crop plants accomplish cell-wall polymer O-acetylation are largely unknown. Here, we report on the isolation and characterization of two trichome birefringence-like (tbl) mutants in rice, which are affected in xylan O-acetylation. ostbl1 and ostbl2 single mutant and the tbl1 tbl2 double mutant displayed a stunted growth phenotype with varied degree of dwarfism. As shown by chemical assays, the wall acetylation level is affected in the mutants and the knock-down and overexpression transgenic plants. Furthermore, nuclear magnetic resonance (NMR) spectroscopy analyses showed that all those mutants have varied decreases in xylan monoacetylation. The divergent expression level of OsTBL1 and OsTBL2 explained the chemotype difference and indicated that OsTBL1 is a functionally dominant gene. OsTBL1 was found to be Golgi-localized. The recombinant OsTBL1 protein incorporates acetyl groups onto xylan. By using xylopentaose (X5), a preferring acceptor substrate, OsTBL1 can transfer up to four acetyl residues onto X5 and this activity showed saturable kinetics. 2D-NMR spectroscopy specified that OsTBL1 transfers acetate to both 2-O and 3-O site of xylosyl residues. In addition, ostbl1 and tbl1 tbl2 displayed susceptibility to rice blight disease, indicating that this xylan modification is required for pathogen resistance. This study offers us the insights into the mechanisms and functions of xylan acetylation in crop plants. |
刊物名称: |
Plant Physiology |
英文刊物名称: |
Plant Physiology |
论文全文: |
|
英文论文全文: |
|
全文链接: |
|
其它备注: |
Yaping Gao, Congwu He, Dongmei Zhang, Xiangling Liu, Zuopeng Xu, Tian Yanbao, Xue-Hui Liu, Shanshan Zang, Markus Pauly, Yihua Zhou and Baocai Zhang. Two Trichome Birefringence-like proteins Mediate Xylan Acetylation, Which Is Essential for Leaf Blight Resistance in Rice. Plant Physiology. DOI:10.1104/pp.16.01618 |
英文其它备注: |
|
学科: |
|
英文学科: |
|
影响因子: |
|
第一作者所在部门: |
|
英文第一作者所在部门: |
|
论文出处: |
|
英文论文出处: |
|
论文类别: |
|
英文论文类别: |
|
参与作者: |
|
英文参与作者: |
|
|