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Precise Base Editing in Rice, Wheat and Maize with a Cas9- cytidine Deaminase Fusion
Yuan Zong, Yanpeng Wang, Chao Li, Rui Zhang, Kunling Chen, Yidong Ran, Jin-Long Qiu, Daowen Wang, Caixia Gao
Nature Biotechnology
Abstract
Targeted base editing in plants without the need for a foreign DNA donor or double-stranded DNA cleavage would accelerate genome modification and breeding in a wide array of crops. We used a CRISPR–Cas9 nickase-cytidine deaminase fusion to achieve targeted conversion of cytosine to thymine from position 3 to 9 within the protospacer in both protoplasts and regenerated rice, wheat and maize plants at frequencies of up to 43.48%
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论文编号: |
DOI:10.1038/nbt.3811 |
论文题目: |
Precise Base Editing in Rice, Wheat and Maize with a Cas9- cytidine Deaminase Fusion |
英文论文题目: |
Precise Base Editing in Rice, Wheat and Maize with a Cas9- cytidine Deaminase Fusion |
第一作者: |
Yuan Zong, Yanpeng Wang, Chao Li, Rui Zhang, Kunling Chen, Yidong Ran, Jin-Long Qiu, Daowen Wang, Caixia Gao |
英文第一作者: |
Yuan Zong, Yanpeng Wang, Chao Li, Rui Zhang, Kunling Chen, Yidong Ran, Jin-Long Qiu, Daowen Wang, Caixia Gao |
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2017-02-28 |
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Targeted base editing in plants without the need for a foreign DNA donor or double-stranded DNA cleavage would accelerate genome modification and breeding in a wide array of crops. We used a CRISPR–Cas9 nickase-cytidine deaminase fusion to achieve targeted conversion of cytosine to thymine from position 3 to 9 within the protospacer in both protoplasts and regenerated rice, wheat and maize plants at frequencies of up to 43.48% |
英文摘要: |
Targeted base editing in plants without the need for a foreign DNA donor or double-stranded DNA cleavage would accelerate genome modification and breeding in a wide array of crops. We used a CRISPR–Cas9 nickase-cytidine deaminase fusion to achieve targeted conversion of cytosine to thymine from position 3 to 9 within the protospacer in both protoplasts and regenerated rice, wheat and maize plants at frequencies of up to 43.48% |
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Nature Biotechnology |
英文刊物名称: |
Nature Biotechnology |
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Yuan Zong, Yanpeng Wang, Chao Li, Rui Zhang, Kunling Chen, Yidong Ran, Jin-Long Qiu, Daowen Wang, Caixia Gao. Precise Base Editing in Rice, Wheat and Maize with a Cas9- cytidine Deaminase Fusion. Nature Biotechnology. DOI:10.1038/nbt.3811 |
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