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Control of Secondary Cell Wall Patterning Involves Xylan Deacetylation by a GDSL Esterase
Baocai Zhang, Lanjun Zhang, Feng Li, Dongmei Zhang, Xiangling Liu, Hang Wang, Zuopeng Xu, Chengcai Chu & Yihua Zhou
Nature Plants
Abstract
O-acetylation, a ubiquitous modification of cell wall polymers, has striking impacts on plant growth and biomass utilization and needs to be tightly controlled. However, the mechanisms that underpin the control of cell wall acetylation remain elusive. Here, we show a ricebrittle leaf sheath1 (bs1) mutant, which contains a lesion in a Golgi-localized GDSL esterase that deacetylates the prominent hemicellulose xylan. Cell wall composition, detailed xylan structure characterization and enzyme kinetics and activity assays on acetylated sugars and xylooligosaccharides demonstrate that BS1 is an esterase that cleaves acetyl moieties from the xylan backbone at O-2 and O-3 positions of xylopyranosyl residues. BS1 thus plays an important role in the maintenance of proper acetylation level on the xylan backbone, which is crucial for secondary wall formation and patterning. Our findings outline a mechanism for how plants modulate wall acetylation and endow a plethora of uncharacterized GDSL esterases with surmisable activities
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DOI:10.1038/nplants.2017.17 |
论文题目: |
Control of Secondary Cell Wall Patterning Involves Xylan Deacetylation by a GDSL Esterase |
英文论文题目: |
Control of Secondary Cell Wall Patterning Involves Xylan Deacetylation by a GDSL Esterase |
第一作者: |
Baocai Zhang, Lanjun Zhang, Feng Li, Dongmei Zhang, Xiangling Liu, Hang Wang, Zuopeng Xu, Chengcai Chu & Yihua Zhou |
英文第一作者: |
Baocai Zhang, Lanjun Zhang, Feng Li, Dongmei Zhang, Xiangling Liu, Hang Wang, Zuopeng Xu, Chengcai Chu & Yihua Zhou |
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2017-03-05 |
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O-acetylation, a ubiquitous modification of cell wall polymers, has striking impacts on plant growth and biomass utilization and needs to be tightly controlled. However, the mechanisms that underpin the control of cell wall acetylation remain elusive. Here, we show a ricebrittle leaf sheath1 (bs1) mutant, which contains a lesion in a Golgi-localized GDSL esterase that deacetylates the prominent hemicellulose xylan. Cell wall composition, detailed xylan structure characterization and enzyme kinetics and activity assays on acetylated sugars and xylooligosaccharides demonstrate that BS1 is an esterase that cleaves acetyl moieties from the xylan backbone at O-2 and O-3 positions of xylopyranosyl residues. BS1 thus plays an important role in the maintenance of proper acetylation level on the xylan backbone, which is crucial for secondary wall formation and patterning. Our findings outline a mechanism for how plants modulate wall acetylation and endow a plethora of uncharacterized GDSL esterases with surmisable activities |
英文摘要: |
O-acetylation, a ubiquitous modification of cell wall polymers, has striking impacts on plant growth and biomass utilization and needs to be tightly controlled. However, the mechanisms that underpin the control of cell wall acetylation remain elusive. Here, we show a ricebrittle leaf sheath1 (bs1) mutant, which contains a lesion in a Golgi-localized GDSL esterase that deacetylates the prominent hemicellulose xylan. Cell wall composition, detailed xylan structure characterization and enzyme kinetics and activity assays on acetylated sugars and xylooligosaccharides demonstrate that BS1 is an esterase that cleaves acetyl moieties from the xylan backbone at O-2 and O-3 positions of xylopyranosyl residues. BS1 thus plays an important role in the maintenance of proper acetylation level on the xylan backbone, which is crucial for secondary wall formation and patterning. Our findings outline a mechanism for how plants modulate wall acetylation and endow a plethora of uncharacterized GDSL esterases with surmisable activities |
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Nature Plants |
英文刊物名称: |
Nature Plants |
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Baocai Zhang, Lanjun Zhang, Feng Li, Dongmei Zhang, Xiangling Liu, Hang Wang, Zuopeng Xu, Chengcai Chu & Yihua Zhou. Control of Secondary Cell Wall Patterning Involves Xylan Deacetylation by a GDSL Esterase. Nature Plants. DOI: 10.1038/nplants.2017.17. |
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