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Ethylene-Inhibited Jasmonic Acid Biosynthesis Promotes Mesocotyl/Coleoptile Elongation of Etiolated Rice Seedlings
Qing Xiong, Biao Ma, Xiang Lu, Yi-Hua Huang, Si-Jie He, Chao Yang, Cui-Cui Yin, He Zhao, Yang Zhou, Wan-Ke Zhang, Wen-Sheng Wang, Zhi-Kang Li, Shou-Yi Chen and Jin-Song Zhang
The Plant Cell
Abstract
Elongation of the mesocotyl and coleoptile facilitates the emergence of rice seedlings from soil, and is affected by various genetic and environment factors. The regulatory mechanism underlying this process remains largely unclear. Here, we examined the regulation of mesocotyl and coleoptile growth by characterizing a gaoyao1 (gy1) mutant that exhibits a longer mesocotyl and longer coleoptile than its original variety of rice (Oryza sativa). GY1 was identified through map-based cloning and encodes a PLA1-type phospholipase that localizes in chloroplasts. GY1 functions at the initial step of jasmonic acid (JA) biosynthesis to repress mesocotyl and coleoptile elongation in etiolated rice seedlings. Ethylene inhibits the expression of GY1 and other genes in the JA biosynthesis pathway to reduce JA levels and enhance mesocotyl and coleoptile growth by promoting cell elongation. Genetically, GY1 acts downstream of the OsEIN2-mediated ethylene signaling pathway to regulate mesocotyl/coleoptile growth. Through analysis of the re-sequencing data from 3,000 rice accessions, we identified a single natural variation of the GY1 gene, GY1376T, which contributes to mesocotyl elongation in rice varieties. Our study reveals novel insights into the regulatory mechanism of mesocotyl/coleoptile elongation, and should have practical applications in rice breeding programs.
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论文编号: |
DOI:10.1105/tpc.16.00981 |
论文题目: |
Ethylene-Inhibited Jasmonic Acid Biosynthesis Promotes Mesocotyl/Coleoptile Elongation of Etiolated Rice Seedlings |
英文论文题目: |
Ethylene-Inhibited Jasmonic Acid Biosynthesis Promotes Mesocotyl/Coleoptile Elongation of Etiolated Rice Seedlings |
第一作者: |
Qing Xiong, Biao Ma, Xiang Lu, Yi-Hua Huang, Si-Jie He, Chao Yang, Cui-Cui Yin, He Zhao, Yang Zhou, Wan-Ke Zhang, Wen-Sheng Wang, Zhi-Kang Li, Shou-Yi Chen and Jin-Song Zhang |
英文第一作者: |
Qing Xiong, Biao Ma, Xiang Lu, Yi-Hua Huang, Si-Jie He, Chao Yang, Cui-Cui Yin, He Zhao, Yang Zhou, Wan-Ke Zhang, Wen-Sheng Wang, Zhi-Kang Li, Shou-Yi Chen and Jin-Song Zhang |
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2017-05-05 |
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摘要: |
Elongation of the mesocotyl and coleoptile facilitates the emergence of rice seedlings from soil, and is affected by various genetic and environment factors. The regulatory mechanism underlying this process remains largely unclear. Here, we examined the regulation of mesocotyl and coleoptile growth by characterizing a gaoyao1 (gy1) mutant that exhibits a longer mesocotyl and longer coleoptile than its original variety of rice (Oryza sativa). GY1 was identified through map-based cloning and encodes a PLA1-type phospholipase that localizes in chloroplasts. GY1 functions at the initial step of jasmonic acid (JA) biosynthesis to repress mesocotyl and coleoptile elongation in etiolated rice seedlings. Ethylene inhibits the expression of GY1 and other genes in the JA biosynthesis pathway to reduce JA levels and enhance mesocotyl and coleoptile growth by promoting cell elongation. Genetically, GY1 acts downstream of the OsEIN2-mediated ethylene signaling pathway to regulate mesocotyl/coleoptile growth. Through analysis of the re-sequencing data from 3,000 rice accessions, we identified a single natural variation of the GY1 gene, GY1376T, which contributes to mesocotyl elongation in rice varieties. Our study reveals novel insights into the regulatory mechanism of mesocotyl/coleoptile elongation, and should have practical applications in rice breeding programs. |
英文摘要: |
Elongation of the mesocotyl and coleoptile facilitates the emergence of rice seedlings from soil, and is affected by various genetic and environment factors. The regulatory mechanism underlying this process remains largely unclear. Here, we examined the regulation of mesocotyl and coleoptile growth by characterizing a gaoyao1 (gy1) mutant that exhibits a longer mesocotyl and longer coleoptile than its original variety of rice (Oryza sativa). GY1 was identified through map-based cloning and encodes a PLA1-type phospholipase that localizes in chloroplasts. GY1 functions at the initial step of jasmonic acid (JA) biosynthesis to repress mesocotyl and coleoptile elongation in etiolated rice seedlings. Ethylene inhibits the expression of GY1 and other genes in the JA biosynthesis pathway to reduce JA levels and enhance mesocotyl and coleoptile growth by promoting cell elongation. Genetically, GY1 acts downstream of the OsEIN2-mediated ethylene signaling pathway to regulate mesocotyl/coleoptile growth. Through analysis of the re-sequencing data from 3,000 rice accessions, we identified a single natural variation of the GY1 gene, GY1376T, which contributes to mesocotyl elongation in rice varieties. Our study reveals novel insights into the regulatory mechanism of mesocotyl/coleoptile elongation, and should have practical applications in rice breeding programs. |
刊物名称: |
The Plant Cell |
英文刊物名称: |
The Plant Cell |
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其它备注: |
Qing Xiong, Biao Ma, Xiang Lu, Yi-Hua Huang, Si-Jie He, Chao Yang, Cui-Cui Yin, He Zhao, Yang Zhou, Wan-Ke Zhang, Wen-Sheng Wang, Zhi-Kang Li, Shou-Yi Chen and Jin-Song Zhang. Ethylene-Inhibited Jasmonic Acid Biosynthesis Promotes Mesocotyl/Coleoptile Elongation of Etiolated Rice Seedlings. The Plant Cell. DOI: 10.1105/tpc.16.00981 |
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