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Translating Divergent Environmental Stresses into a Common Proteome Response through Hik33 in a Model Cyanobacterium
Haitao Ge, Longfa Fang, Xiahe Huang, Jinlong Wang, Weiyang Chen, Ye Liu, Yuanya Zhang, Xiaorong Wang, Wu Xu, Qingfang He, and Yingchun Wang
Molecular & Cellular Proteomics
Abstract
The histidine kinase Hik33 plays important roles in mediating cyanobacterial response to divergent types of abiotic stresses including cold, salt, high light (HL), and osmotic stresses. However, how these functions are regulated by Hik33 remains to be addressed. Using a hik33-deficient strain (Δhik33) of Synechocystis sp. PCC 6803 (Synechocystis) and quantitative proteomics, we found that Hik33 depletion induces differential protein expression highly similar to that induced by divergent types of stresses. This typically includes downregulation of proteins in photosynthesis and carbon assimilation that are necessary for cell propagation, and upregulation of heat shock proteins, chaperons, and proteases that are important for cell survival. This observation indicates that depletion of Hik33 alone mimics divergent types of abiotic stresses, and that Hik33 could be important for preventing abnormal stress response in the normal condition. Moreover, we found the majority of proteins of plasmid origin were significantly upregulated in Δhik33, though their biological significance remains to be addressed. Together, the systematically characterized Hik33-regulated cyanobacterial proteome, which is largely involved in stress responses, builds the molecular basis for Hik33 as a general regulator of stress responses.
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论文编号: |
DOI:10.1074/mcp.M117.068080 |
论文题目: |
Translating Divergent Environmental Stresses into a Common Proteome Response through Hik33 in a Model Cyanobacterium |
英文论文题目: |
Translating Divergent Environmental Stresses into a Common Proteome Response through Hik33 in a Model Cyanobacterium |
第一作者: |
Haitao Ge, Longfa Fang, Xiahe Huang, Jinlong Wang, Weiyang Chen, Ye Liu, Yuanya Zhang, Xiaorong Wang, Wu Xu, Qingfang He, and Yingchun Wang |
英文第一作者: |
Haitao Ge, Longfa Fang, Xiahe Huang, Jinlong Wang, Weiyang Chen, Ye Liu, Yuanya Zhang, Xiaorong Wang, Wu Xu, Qingfang He, and Yingchun Wang |
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2017-05-15 |
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摘要: |
The histidine kinase Hik33 plays important roles in mediating cyanobacterial response to divergent types of abiotic stresses including cold, salt, high light (HL), and osmotic stresses. However, how these functions are regulated by Hik33 remains to be addressed. Using a hik33-deficient strain (Δhik33) of Synechocystis sp. PCC 6803 (Synechocystis) and quantitative proteomics, we found that Hik33 depletion induces differential protein expression highly similar to that induced by divergent types of stresses. This typically includes downregulation of proteins in photosynthesis and carbon assimilation that are necessary for cell propagation, and upregulation of heat shock proteins, chaperons, and proteases that are important for cell survival. This observation indicates that depletion of Hik33 alone mimics divergent types of abiotic stresses, and that Hik33 could be important for preventing abnormal stress response in the normal condition. Moreover, we found the majority of proteins of plasmid origin were significantly upregulated in Δhik33, though their biological significance remains to be addressed. Together, the systematically characterized Hik33-regulated cyanobacterial proteome, which is largely involved in stress responses, builds the molecular basis for Hik33 as a general regulator of stress responses. |
英文摘要: |
The histidine kinase Hik33 plays important roles in mediating cyanobacterial response to divergent types of abiotic stresses including cold, salt, high light (HL), and osmotic stresses. However, how these functions are regulated by Hik33 remains to be addressed. Using a hik33-deficient strain (Δhik33) of Synechocystis sp. PCC 6803 (Synechocystis) and quantitative proteomics, we found that Hik33 depletion induces differential protein expression highly similar to that induced by divergent types of stresses. This typically includes downregulation of proteins in photosynthesis and carbon assimilation that are necessary for cell propagation, and upregulation of heat shock proteins, chaperons, and proteases that are important for cell survival. This observation indicates that depletion of Hik33 alone mimics divergent types of abiotic stresses, and that Hik33 could be important for preventing abnormal stress response in the normal condition. Moreover, we found the majority of proteins of plasmid origin were significantly upregulated in Δhik33, though their biological significance remains to be addressed. Together, the systematically characterized Hik33-regulated cyanobacterial proteome, which is largely involved in stress responses, builds the molecular basis for Hik33 as a general regulator of stress responses. |
刊物名称: |
Molecular & Cellular Proteomics |
英文刊物名称: |
Molecular & Cellular Proteomics |
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Haitao Ge, Longfa Fang, Xiahe Huang, Jinlong Wang, Weiyang Chen, Ye Liu, Yuanya Zhang, Xiaorong Wang, Wu Xu, Qingfang He, and Yingchun Wang. Translating Divergent Environmental Stresses into a Common Proteome Response through Hik33 in a Model Cyanobacterium. Molecular & Cellular Proteomics. DOI: 10.1074/mcp.M117.068080 |
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