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CAMSAP3 Accumulates in the Pericentrosomal Area and Accompanies Microtubules Release from the Centrosome via Katanin
Congcong Dong, Honglin Xu, Rui Zhang, Nobutoshi Tanaka, Masatoshi Takeichi, Wenxiang Meng
Journal of Cell Science
Abstract
The epithelium has an apico-basal axis polarity that plays an important role in absorption, excretion and other physiological functions. In epithelial cells, a substantial number of non-centrosomal microtubules (MTs) are scattered in the cytoplasm with an apico-basal polarity and reorientate as epithelial cells perform different functions. Several previous studies have found that non-centrosomal MTs are nucleated at the centrosome, and then released and translocated elsewhere. However, the detailed process and molecular mechanism remain largely unknown. In this study, we found that Nezha, also called calmodulin-regulated spectrin-associated protein 3 (CAMSAP3), a non-centrosomal MT minus-end protein, accumulates in the pericentrosomal area and accompanies the release of MTs from the centrosome; whereas depletion of CAMSAP3 prevented MT release and instead caused focusing of MTs at centrosomes. Further studies demonstrated that CAMSAP3 precisely coordinates with dynein and katanin to regulate the MT detachment process. In conclusion, our results indicate that CAMSAP3 is a key molecule for generation of non-centrosomal MTs.
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论文编号: |
DOI:10.1242/jcs.198010 |
论文题目: |
CAMSAP3 Accumulates in the Pericentrosomal Area and Accompanies Microtubules Release from the Centrosome via Katanin |
英文论文题目: |
CAMSAP3 Accumulates in the Pericentrosomal Area and Accompanies Microtubules Release from the Centrosome via Katanin |
第一作者: |
Congcong Dong, Honglin Xu, Rui Zhang, Nobutoshi Tanaka, Masatoshi Takeichi, Wenxiang Meng |
英文第一作者: |
Congcong Dong, Honglin Xu, Rui Zhang, Nobutoshi Tanaka, Masatoshi Takeichi, Wenxiang Meng |
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2017-05-25 |
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摘要: |
The epithelium has an apico-basal axis polarity that plays an important role in absorption, excretion and other physiological functions. In epithelial cells, a substantial number of non-centrosomal microtubules (MTs) are scattered in the cytoplasm with an apico-basal polarity and reorientate as epithelial cells perform different functions. Several previous studies have found that non-centrosomal MTs are nucleated at the centrosome, and then released and translocated elsewhere. However, the detailed process and molecular mechanism remain largely unknown. In this study, we found that Nezha, also called calmodulin-regulated spectrin-associated protein 3 (CAMSAP3), a non-centrosomal MT minus-end protein, accumulates in the pericentrosomal area and accompanies the release of MTs from the centrosome; whereas depletion of CAMSAP3 prevented MT release and instead caused focusing of MTs at centrosomes. Further studies demonstrated that CAMSAP3 precisely coordinates with dynein and katanin to regulate the MT detachment process. In conclusion, our results indicate that CAMSAP3 is a key molecule for generation of non-centrosomal MTs. |
英文摘要: |
The epithelium has an apico-basal axis polarity that plays an important role in absorption, excretion and other physiological functions. In epithelial cells, a substantial number of non-centrosomal microtubules (MTs) are scattered in the cytoplasm with an apico-basal polarity and reorientate as epithelial cells perform different functions. Several previous studies have found that non-centrosomal MTs are nucleated at the centrosome, and then released and translocated elsewhere. However, the detailed process and molecular mechanism remain largely unknown. In this study, we found that Nezha, also called calmodulin-regulated spectrin-associated protein 3 (CAMSAP3), a non-centrosomal MT minus-end protein, accumulates in the pericentrosomal area and accompanies the release of MTs from the centrosome; whereas depletion of CAMSAP3 prevented MT release and instead caused focusing of MTs at centrosomes. Further studies demonstrated that CAMSAP3 precisely coordinates with dynein and katanin to regulate the MT detachment process. In conclusion, our results indicate that CAMSAP3 is a key molecule for generation of non-centrosomal MTs. |
刊物名称: |
Journal of Cell Science |
英文刊物名称: |
Journal of Cell Science |
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其它备注: |
Congcong Dong, Honglin Xu, Rui Zhang, Nobutoshi Tanaka, Masatoshi Takeichi, Wenxiang Meng. CAMSAP3 Accumulates in the Pericentrosomal Area and Accompanies Microtubules Release from the Centrosome via Katanin. Journal of Cell Science. DOI: 10.1242/jcs.198010 |
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