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Cohesion and Centromere Activity Are Required for Histone H3 Phosphorylation in Maize
Yang Liu, Handong Su, Yalin Liu, Jing Zhang, Qianhua Dong, James A. Birchler, Fangpu Han
The Plant Journal
Abstract
Haspin-mediated histone H3-threonine 3 phosphorylation (H3T3ph) promotes proper deposition of Aurora B at the inner centromere to ensure faithful chromosome segregation in metazoans. However, the function of H3T3ph remains relatively unexplored in plants. Here, we show that in maize (Zea mays L.) mitotic cells, H3T3ph is concentrated at pericentromeric and centromeric regions. Additional weak H3T3ph signals occur between cohered sister chromatids at prometaphase. Immunostaining on dicentric chromosomes reveals that an inactive centromere cannot maintain H3T3ph at metaphase, indicating that a functional centromere is required for H3T3 phosphorylation. H3T3ph locates at a newly formed centromeric region that lacks detectable CentC sequences and strongly reduced CRM and ZmBs repeat sequences at metaphase II. These results suggest that centromeric localization of H3T3ph is not dependent on centromeric sequences. In maize meiocytes, H3T3 phosphorylation occurs at the late diakinesis and extends to the entire chromosome at metaphase I, but is exclusively limited to the centromere at metaphase II. The H3T3ph signals are absent in the afd1 (absence of first division) and sgo1 (shugoshin) mutants during meiosis II when the sister chromatids exhibit random distribution. Further, we showed that H3T3ph is mainly located at the pericentromere during meiotic prophase II, but is restricted to the inner centromere at metaphase II. We propose that this H3T3ph relocation depends on tension at centromere and is required to promote bi-orientation of sister chromatids.
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论文编号: |
DOI:10.1111/tpj.13748 |
论文题目: |
Cohesion and Centromere Activity Are Required for Histone H3 Phosphorylation in Maize |
英文论文题目: |
Cohesion and Centromere Activity Are Required for Histone H3 Phosphorylation in Maize |
第一作者: |
Yang Liu, Handong Su, Yalin Liu, Jing Zhang, Qianhua Dong, James A. Birchler, Fangpu Han |
英文第一作者: |
Yang Liu, Handong Su, Yalin Liu, Jing Zhang, Qianhua Dong, James A. Birchler, Fangpu Han |
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2017-10-16 |
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摘要: |
Haspin-mediated histone H3-threonine 3 phosphorylation (H3T3ph) promotes proper deposition of Aurora B at the inner centromere to ensure faithful chromosome segregation in metazoans. However, the function of H3T3ph remains relatively unexplored in plants. Here, we show that in maize (Zea mays L.) mitotic cells, H3T3ph is concentrated at pericentromeric and centromeric regions. Additional weak H3T3ph signals occur between cohered sister chromatids at prometaphase. Immunostaining on dicentric chromosomes reveals that an inactive centromere cannot maintain H3T3ph at metaphase, indicating that a functional centromere is required for H3T3 phosphorylation. H3T3ph locates at a newly formed centromeric region that lacks detectable CentC sequences and strongly reduced CRM and ZmBs repeat sequences at metaphase II. These results suggest that centromeric localization of H3T3ph is not dependent on centromeric sequences. In maize meiocytes, H3T3 phosphorylation occurs at the late diakinesis and extends to the entire chromosome at metaphase I, but is exclusively limited to the centromere at metaphase II. The H3T3ph signals are absent in the afd1 (absence of first division) and sgo1 (shugoshin) mutants during meiosis II when the sister chromatids exhibit random distribution. Further, we showed that H3T3ph is mainly located at the pericentromere during meiotic prophase II, but is restricted to the inner centromere at metaphase II. We propose that this H3T3ph relocation depends on tension at centromere and is required to promote bi-orientation of sister chromatids. |
英文摘要: |
Haspin-mediated histone H3-threonine 3 phosphorylation (H3T3ph) promotes proper deposition of Aurora B at the inner centromere to ensure faithful chromosome segregation in metazoans. However, the function of H3T3ph remains relatively unexplored in plants. Here, we show that in maize (Zea mays L.) mitotic cells, H3T3ph is concentrated at pericentromeric and centromeric regions. Additional weak H3T3ph signals occur between cohered sister chromatids at prometaphase. Immunostaining on dicentric chromosomes reveals that an inactive centromere cannot maintain H3T3ph at metaphase, indicating that a functional centromere is required for H3T3 phosphorylation. H3T3ph locates at a newly formed centromeric region that lacks detectable CentC sequences and strongly reduced CRM and ZmBs repeat sequences at metaphase II. These results suggest that centromeric localization of H3T3ph is not dependent on centromeric sequences. In maize meiocytes, H3T3 phosphorylation occurs at the late diakinesis and extends to the entire chromosome at metaphase I, but is exclusively limited to the centromere at metaphase II. The H3T3ph signals are absent in the afd1 (absence of first division) and sgo1 (shugoshin) mutants during meiosis II when the sister chromatids exhibit random distribution. Further, we showed that H3T3ph is mainly located at the pericentromere during meiotic prophase II, but is restricted to the inner centromere at metaphase II. We propose that this H3T3ph relocation depends on tension at centromere and is required to promote bi-orientation of sister chromatids. |
刊物名称: |
The Plant Journal |
英文刊物名称: |
The Plant Journal |
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其它备注: |
Yang Liu, Handong Su, Yalin Liu, Jing Zhang, Qianhua Dong, James A. Birchler, Fangpu Han. Cohesion and Centromere Activity Are Required for Histone H3 Phosphorylation in Maize. The Plant Journal. DOI: 10.1111/tpj.13748 |
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