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AUXIN RESPONSE FACTOR3 Regulates Floral Meristem Determinacy By Repressing Cytokinin Biosynthesis and Signaling
Ke Zhang, Ruozhong Wang, Hailing Zi, Yongpeng Li, Xiuwei Cao, Dongming Li, Lin Guo, Jianhua Tong, Yanyun Pan, Yuling Jiao, Renyi Liu, Langtao Xiao, Xigang Liu
The Plant Cell
Abstract
Successful floral meristem (FM) determinacy is critical for subsequent reproductive development and the plant life cycle. Although the phytohormones cytokinin and auxin interact to coregulate many aspects of plant development, whether and how cytokinin and auxin function in FM determinacy remain unclear. Here we show that in Arabidopsis thaliana, cytokinin homeostasis is critical for FM determinacy. In this developmental context, auxin promotes the expression of AUXIN RESPONSE FACTOR3 (ARF3) to repress cytokinin activity. ARF3 directly represses the expression of ISOPENTENYLTRANSFERASE (IPT) family genes and indirectly represses LONELY GUY (LOG) family genes, both of which encode enzymes required for cytokinin biosynthesis. ARF3 also directly inhibits the expression of ARABIDOPSIS HISTIDINE KINASE4 (AHK4), a cytokinin receptor gene, resulting in reduced cytokinin activity. Consequently, ARF3 controls cell division by regulating cell cycle gene expression through cytokinin. In flowers, we show that AGAMOUS (AG) dynamically regulates the expression of ARF3 and IPTs, resulting in coordinated regulation of FM maintenance and termination through cell division. Moreover, genome-wide transcriptional profiling revealed both repressive and active roles for ARF3 in early flower development. Our findings establish a molecular link between AG and auxin/cytokinin and shed light on the mechanisms of stem cell maintenance and termination in the FM.
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论文编号: |
DOI:10.1105/tpc.17.00705 |
论文题目: |
AUXIN RESPONSE FACTOR3 Regulates Floral Meristem Determinacy By Repressing Cytokinin Biosynthesis and Signaling |
英文论文题目: |
AUXIN RESPONSE FACTOR3 Regulates Floral Meristem Determinacy By Repressing Cytokinin Biosynthesis and Signaling |
第一作者: |
Ke Zhang, Ruozhong Wang, Hailing Zi, Yongpeng Li, Xiuwei Cao, Dongming Li, Lin Guo, Jianhua Tong, Yanyun Pan, Yuling Jiao, Renyi Liu, Langtao Xiao, Xigang Liu |
英文第一作者: |
Ke Zhang, Ruozhong Wang, Hailing Zi, Yongpeng Li, Xiuwei Cao, Dongming Li, Lin Guo, Jianhua Tong, Yanyun Pan, Yuling Jiao, Renyi Liu, Langtao Xiao, Xigang Liu |
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2018-01-25 |
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Successful floral meristem (FM) determinacy is critical for subsequent reproductive development and the plant life cycle. Although the phytohormones cytokinin and auxin interact to coregulate many aspects of plant development, whether and how cytokinin and auxin function in FM determinacy remain unclear. Here we show that in Arabidopsis thaliana, cytokinin homeostasis is critical for FM determinacy. In this developmental context, auxin promotes the expression of AUXIN RESPONSE FACTOR3 (ARF3) to repress cytokinin activity. ARF3 directly represses the expression of ISOPENTENYLTRANSFERASE (IPT) family genes and indirectly represses LONELY GUY (LOG) family genes, both of which encode enzymes required for cytokinin biosynthesis. ARF3 also directly inhibits the expression of ARABIDOPSIS HISTIDINE KINASE4 (AHK4), a cytokinin receptor gene, resulting in reduced cytokinin activity. Consequently, ARF3 controls cell division by regulating cell cycle gene expression through cytokinin. In flowers, we show that AGAMOUS (AG) dynamically regulates the expression of ARF3 and IPTs, resulting in coordinated regulation of FM maintenance and termination through cell division. Moreover, genome-wide transcriptional profiling revealed both repressive and active roles for ARF3 in early flower development. Our findings establish a molecular link between AG and auxin/cytokinin and shed light on the mechanisms of stem cell maintenance and termination in the FM. |
英文摘要: |
Successful floral meristem (FM) determinacy is critical for subsequent reproductive development and the plant life cycle. Although the phytohormones cytokinin and auxin interact to coregulate many aspects of plant development, whether and how cytokinin and auxin function in FM determinacy remain unclear. Here we show that in Arabidopsis thaliana, cytokinin homeostasis is critical for FM determinacy. In this developmental context, auxin promotes the expression of AUXIN RESPONSE FACTOR3 (ARF3) to repress cytokinin activity. ARF3 directly represses the expression of ISOPENTENYLTRANSFERASE (IPT) family genes and indirectly represses LONELY GUY (LOG) family genes, both of which encode enzymes required for cytokinin biosynthesis. ARF3 also directly inhibits the expression of ARABIDOPSIS HISTIDINE KINASE4 (AHK4), a cytokinin receptor gene, resulting in reduced cytokinin activity. Consequently, ARF3 controls cell division by regulating cell cycle gene expression through cytokinin. In flowers, we show that AGAMOUS (AG) dynamically regulates the expression of ARF3 and IPTs, resulting in coordinated regulation of FM maintenance and termination through cell division. Moreover, genome-wide transcriptional profiling revealed both repressive and active roles for ARF3 in early flower development. Our findings establish a molecular link between AG and auxin/cytokinin and shed light on the mechanisms of stem cell maintenance and termination in the FM. |
刊物名称: |
The Plant Cell |
英文刊物名称: |
The Plant Cell |
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其它备注: |
Ke Zhang, Ruozhong Wang, Hailing Zi, Yongpeng Li, Xiuwei Cao, Dongming Li, Lin Guo, Jianhua Tong, Yanyun Pan, Yuling Jiao, Renyi Liu, Langtao Xiao, Xigang Liu. AUXIN RESPONSE FACTOR3 Regulates Floral Meristem Determinacy By Repressing Cytokinin Biosynthesis and Signaling. The Plant Cell. DOI:10.1105/tpc.17.00705 |
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