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OsSPL Regulates Meiotic Fate Acquisition in Rice
Lijun Ren, Ding Tang, Tingting Zhao, Fanfan Zhang, Changzhen Liu, Zhihui Xue, Wenqing Shi, Guijie Du, Yi Shen, Yafei Li, Zhukuan Cheng
New Phytologist
Abstract
In angiosperms, the key step in sexual reproduction is successful acquisition of meiotic fate. However, the molecular mechanism determining meiotic fate remains largely unknown. Here, we report that OsSPOROCYTELESS (OsSPL) is critical for meiotic entry in rice (Oryza sativa). We performed a large-scale genetic screen of rice sterile mutants aimed to identify genes regulating meiotic entry and identified OsSPL using map-based cloning. We showed that meiosis-specific callose deposition, chromatin organization, and centromere-specific histone H3 loading were altered in the cells corresponding to pollen mother cells in Osspl anthers. Global transcriptome analysis showed that the enriched differentially expressed genes in Osspl were mainly related to redox status, meiotic process, and parietal cell development. OsSPL might form homodimers and interact with TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) transcription factor OsTCP5 via the SPL dimerization and TCP interaction domain. OsSPL also interacts with TPL (TOPLESS) corepressors, OsTPL2 and OsTPL3, via the EAR motif. Our results suggest that the OsSPL-mediated signaling pathway plays a crucial role in rice meiotic entry, which appears to be a conserved regulatory mechanism for meiotic fate acquisition in angiosperms.
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论文编号: |
DOI:10.1111/nph.15017 |
论文题目: |
OsSPL Regulates Meiotic Fate Acquisition in Rice |
英文论文题目: |
OsSPL Regulates Meiotic Fate Acquisition in Rice |
第一作者: |
Lijun Ren, Ding Tang, Tingting Zhao, Fanfan Zhang, Changzhen Liu, Zhihui Xue, Wenqing Shi, Guijie Du, Yi Shen, Yafei Li, Zhukuan Cheng |
英文第一作者: |
Lijun Ren, Ding Tang, Tingting Zhao, Fanfan Zhang, Changzhen Liu, Zhihui Xue, Wenqing Shi, Guijie Du, Yi Shen, Yafei Li, Zhukuan Cheng |
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2018-02-27 |
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摘要: |
In angiosperms, the key step in sexual reproduction is successful acquisition of meiotic fate. However, the molecular mechanism determining meiotic fate remains largely unknown. Here, we report that OsSPOROCYTELESS (OsSPL) is critical for meiotic entry in rice (Oryza sativa). We performed a large-scale genetic screen of rice sterile mutants aimed to identify genes regulating meiotic entry and identified OsSPL using map-based cloning. We showed that meiosis-specific callose deposition, chromatin organization, and centromere-specific histone H3 loading were altered in the cells corresponding to pollen mother cells in Osspl anthers. Global transcriptome analysis showed that the enriched differentially expressed genes in Osspl were mainly related to redox status, meiotic process, and parietal cell development. OsSPL might form homodimers and interact with TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) transcription factor OsTCP5 via the SPL dimerization and TCP interaction domain. OsSPL also interacts with TPL (TOPLESS) corepressors, OsTPL2 and OsTPL3, via the EAR motif. Our results suggest that the OsSPL-mediated signaling pathway plays a crucial role in rice meiotic entry, which appears to be a conserved regulatory mechanism for meiotic fate acquisition in angiosperms. |
英文摘要: |
In angiosperms, the key step in sexual reproduction is successful acquisition of meiotic fate. However, the molecular mechanism determining meiotic fate remains largely unknown. Here, we report that OsSPOROCYTELESS (OsSPL) is critical for meiotic entry in rice (Oryza sativa). We performed a large-scale genetic screen of rice sterile mutants aimed to identify genes regulating meiotic entry and identified OsSPL using map-based cloning. We showed that meiosis-specific callose deposition, chromatin organization, and centromere-specific histone H3 loading were altered in the cells corresponding to pollen mother cells in Osspl anthers. Global transcriptome analysis showed that the enriched differentially expressed genes in Osspl were mainly related to redox status, meiotic process, and parietal cell development. OsSPL might form homodimers and interact with TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) transcription factor OsTCP5 via the SPL dimerization and TCP interaction domain. OsSPL also interacts with TPL (TOPLESS) corepressors, OsTPL2 and OsTPL3, via the EAR motif. Our results suggest that the OsSPL-mediated signaling pathway plays a crucial role in rice meiotic entry, which appears to be a conserved regulatory mechanism for meiotic fate acquisition in angiosperms. |
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New Phytologist |
英文刊物名称: |
New Phytologist |
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其它备注: |
Lijun Ren, Ding Tang, Tingting Zhao, Fanfan Zhang, Changzhen Liu, Zhihui Xue, Wenqing Shi, Guijie Du, Yi Shen, Yafei Li, Zhukuan Cheng. OsSPL Regulates Meiotic Fate Acquisition in Rice. New Phytologist. DOI:10.1111/nph.15017 |
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