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Endosperm Sugar Accumulation Caused by Mutation of PHS8/ISA1 Leads to Pre-harvest Sprouting in Rice
Lin Du, Fan Xu, Jun Fang, Shaopei Gao, Jiuyou Tang, Shuang Fang, Hongru Wang, Hongning Tong, Fengxia Zhang, Jinfang Chu, Guodong Wang, Chengcai Chu
The Plant Journal
Abstract
Pre‐harvest sprouting (PHS) is an unfavorable trait in cereal crops which could seriously decrease grain yield and quality. Although some PHS‐associated quantitative trait loci (QTL) or genes in cereals have been reported, the molecular mechanism underlying PHS remains largely elusive. Here, we characterized a rice mutant, phs8, which exhibits PHS phenotype accompanied by sugary endosperm. Map‐based cloning revealed that PHS8encodes a starch debranching enzyme named isoamylase1. Mutation in PHS8 resulted in the phytoglycogen breakdown and sugar accumulation in the endosperm. Intriguingly, with increase of sugar contents, decreased expression of OsABI3 and OsABI5 as well as reduced sensitivity to ABA were found in the phs8 mutant. Using rice suspension cell system, we confirmed that exogenous sugar is sufficient to suppress the expression of both OsABI3 and OsABI5. Furthermore, overexpression of OsABI3 or OsABI5 could partially rescue the PHS phenotype of phs8. Therefore, our study presents important evidence supporting that endosperm sugar not only acts as an essential energy source for seed germination but also determines seed dormancy and germination by affecting ABA signaling.
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论文编号: |
DOI:10.1111/tpj.13970 |
论文题目: |
Endosperm Sugar Accumulation Caused by Mutation of PHS8/ISA1 Leads to Pre-harvest Sprouting in Rice |
英文论文题目: |
Endosperm Sugar Accumulation Caused by Mutation of PHS8/ISA1 Leads to Pre-harvest Sprouting in Rice |
第一作者: |
Lin Du, Fan Xu, Jun Fang, Shaopei Gao, Jiuyou Tang, Shuang Fang, Hongru Wang, Hongning Tong, Fengxia Zhang, Jinfang Chu, Guodong Wang, Chengcai Chu |
英文第一作者: |
Lin Du, Fan Xu, Jun Fang, Shaopei Gao, Jiuyou Tang, Shuang Fang, Hongru Wang, Hongning Tong, Fengxia Zhang, Jinfang Chu, Guodong Wang, Chengcai Chu |
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2018-05-23 |
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摘要: |
Pre‐harvest sprouting (PHS) is an unfavorable trait in cereal crops which could seriously decrease grain yield and quality. Although some PHS‐associated quantitative trait loci (QTL) or genes in cereals have been reported, the molecular mechanism underlying PHS remains largely elusive. Here, we characterized a rice mutant, phs8, which exhibits PHS phenotype accompanied by sugary endosperm. Map‐based cloning revealed that PHS8encodes a starch debranching enzyme named isoamylase1. Mutation in PHS8 resulted in the phytoglycogen breakdown and sugar accumulation in the endosperm. Intriguingly, with increase of sugar contents, decreased expression of OsABI3 and OsABI5 as well as reduced sensitivity to ABA were found in the phs8 mutant. Using rice suspension cell system, we confirmed that exogenous sugar is sufficient to suppress the expression of both OsABI3 and OsABI5. Furthermore, overexpression of OsABI3 or OsABI5 could partially rescue the PHS phenotype of phs8. Therefore, our study presents important evidence supporting that endosperm sugar not only acts as an essential energy source for seed germination but also determines seed dormancy and germination by affecting ABA signaling. |
英文摘要: |
Pre‐harvest sprouting (PHS) is an unfavorable trait in cereal crops which could seriously decrease grain yield and quality. Although some PHS‐associated quantitative trait loci (QTL) or genes in cereals have been reported, the molecular mechanism underlying PHS remains largely elusive. Here, we characterized a rice mutant, phs8, which exhibits PHS phenotype accompanied by sugary endosperm. Map‐based cloning revealed that PHS8encodes a starch debranching enzyme named isoamylase1. Mutation in PHS8 resulted in the phytoglycogen breakdown and sugar accumulation in the endosperm. Intriguingly, with increase of sugar contents, decreased expression of OsABI3 and OsABI5 as well as reduced sensitivity to ABA were found in the phs8 mutant. Using rice suspension cell system, we confirmed that exogenous sugar is sufficient to suppress the expression of both OsABI3 and OsABI5. Furthermore, overexpression of OsABI3 or OsABI5 could partially rescue the PHS phenotype of phs8. Therefore, our study presents important evidence supporting that endosperm sugar not only acts as an essential energy source for seed germination but also determines seed dormancy and germination by affecting ABA signaling. |
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The Plant Journal |
英文刊物名称: |
The Plant Journal |
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其它备注: |
Lin Du, Fan Xu, Jun Fang, Shaopei Gao, Jiuyou Tang, Shuang Fang, Hongru Wang, Hongning Tong, Fengxia Zhang, Jinfang Chu, Guodong Wang, Chengcai Chu. Endosperm Sugar Accumulation Caused by Mutation of PHS8/ISA1 Leads to Pre-harvest Sprouting in Rice. The Plant Journal. DOI:10.1111/tpj.13970 |
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