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Expanded Base Editing in Rice and Wheat Using a Cas9-adenosine Deaminase Fusion
Chao Li, Yuan Zong, Yanpeng Wang, Shuai Jin, Dingbo Zhang, Qianna Song, Rui Zhang and Caixia Gao
Genome Biology
Abstract
Nucleotide base editors in plants have been limited to conversion of cytosine to thymine. Here, we describe a new plant adenine base editor based on an evolved tRNA adenosine deaminase fused to the nickase CRISPR/Cas9, enabling A?T to G?C conversion at frequencies up to 7.5% in protoplasts and 59.1% in regenerated rice and wheat plants. An endogenous gene is also successfully modified through introducing a gain-of-function point mutation to directly produce an herbicide-tolerant rice plant. With this new adenine base editing system, it is now possible to precisely edit all base pairs, thus expanding the toolset for precise editing in plants.
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论文编号: |
DOI:10.1186/s13059-018-1443-z |
论文题目: |
Expanded Base Editing in Rice and Wheat Using a Cas9-adenosine Deaminase Fusion |
英文论文题目: |
Expanded Base Editing in Rice and Wheat Using a Cas9-adenosine Deaminase Fusion |
第一作者: |
Chao Li, Yuan Zong, Yanpeng Wang, Shuai Jin, Dingbo Zhang, Qianna Song, Rui Zhang and Caixia Gao |
英文第一作者: |
Chao Li, Yuan Zong, Yanpeng Wang, Shuai Jin, Dingbo Zhang, Qianna Song, Rui Zhang and Caixia Gao |
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2018-05-30 |
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摘要: |
Nucleotide base editors in plants have been limited to conversion of cytosine to thymine. Here, we describe a new plant adenine base editor based on an evolved tRNA adenosine deaminase fused to the nickase CRISPR/Cas9, enabling A?T to G?C conversion at frequencies up to 7.5% in protoplasts and 59.1% in regenerated rice and wheat plants. An endogenous gene is also successfully modified through introducing a gain-of-function point mutation to directly produce an herbicide-tolerant rice plant. With this new adenine base editing system, it is now possible to precisely edit all base pairs, thus expanding the toolset for precise editing in plants. |
英文摘要: |
Nucleotide base editors in plants have been limited to conversion of cytosine to thymine. Here, we describe a new plant adenine base editor based on an evolved tRNA adenosine deaminase fused to the nickase CRISPR/Cas9, enabling A?T to G?C conversion at frequencies up to 7.5% in protoplasts and 59.1% in regenerated rice and wheat plants. An endogenous gene is also successfully modified through introducing a gain-of-function point mutation to directly produce an herbicide-tolerant rice plant. With this new adenine base editing system, it is now possible to precisely edit all base pairs, thus expanding the toolset for precise editing in plants. |
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Genome Biology |
英文刊物名称: |
Genome Biology |
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Chao Li, Yuan Zong, Yanpeng Wang, Shuai Jin, Dingbo Zhang, Qianna Song, Rui Zhang and Caixia Gao. Expanded Base Editing in Rice and Wheat Using a Cas9-Adenosine Deaminase Fusion. Genome Biology. DOI:10.1186/s13059-018-1443-z |
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