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Efficient C-to-T base Editing in Plants Using a Fusion of nCas9 and Human APOBEC3A
Yuan Zong, Qianna Song, Chao Li, Shuai Jin, Dingbo Zhang, Yanpeng Wang, Jin-Long Qiu & Caixia Gao
Nature Biotechnology
Abstract
Base editors (BEs) have been used to create C-to-T substitutions in various organisms. However, editing with rat APOBEC1-based BE3 is limited to a 5-nt sequence editing window and is inefficient in GC contexts. Here, we show that a base editor fusion protein composed of Cas9 nickase and human APOBEC3A (A3A-PBE) converts cytidine to thymidine efficiently in wheat, rice and potato with a 17-nucleotide editing window at all examined sites, independent of sequence context.
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论文编号: |
DOI:10.1038/nbt.4261 |
论文题目: |
Efficient C-to-T base Editing in Plants Using a Fusion of nCas9 and Human APOBEC3A |
英文论文题目: |
Efficient C-to-T base Editing in Plants Using a Fusion of nCas9 and Human APOBEC3A |
第一作者: |
Yuan Zong, Qianna Song, Chao Li, Shuai Jin, Dingbo Zhang, Yanpeng Wang, Jin-Long Qiu & Caixia Gao |
英文第一作者: |
Yuan Zong, Qianna Song, Chao Li, Shuai Jin, Dingbo Zhang, Yanpeng Wang, Jin-Long Qiu & Caixia Gao |
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2018-10-02 |
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摘要: |
Base editors (BEs) have been used to create C-to-T substitutions in various organisms. However, editing with rat APOBEC1-based BE3 is limited to a 5-nt sequence editing window and is inefficient in GC contexts. Here, we show that a base editor fusion protein composed of Cas9 nickase and human APOBEC3A (A3A-PBE) converts cytidine to thymidine efficiently in wheat, rice and potato with a 17-nucleotide editing window at all examined sites, independent of sequence context. |
英文摘要: |
Base editors (BEs) have been used to create C-to-T substitutions in various organisms. However, editing with rat APOBEC1-based BE3 is limited to a 5-nt sequence editing window and is inefficient in GC contexts. Here, we show that a base editor fusion protein composed of Cas9 nickase and human APOBEC3A (A3A-PBE) converts cytidine to thymidine efficiently in wheat, rice and potato with a 17-nucleotide editing window at all examined sites, independent of sequence context. |
刊物名称: |
Nature Biotechnology |
英文刊物名称: |
Nature Biotechnology |
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Yuan Zong, Qianna Song, Chao Li, Shuai Jin, Dingbo Zhang, Yanpeng Wang, Jin-Long Qiu & Caixia Gao. Efficient C-to-T base Editing in Plants Using a Fusion of nCas9 and Human APOBEC3A. Nature Biotechnology. DOI: 10.1038/nbt.4261 |
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