|
The Glycosphingolipid MacCer Promotes Synaptic Bouton Formation in Drosophila by Interacting with Wnt
Yan Huang, Sheng Huang, Coralie Di Scala, Qifu Wang, Hans H Wandall, Jacques Fantini, Yong Q Zhang
eLIFE
Abstract
Lipids are structural components of cellular membranes and signaling molecules that are widely involved in development and diseases, but the underlying molecular mechanisms are poorly understood, partly because of the vast variety of lipid species and complexity of synthetic and turnover pathways. From a genetic screen, we identify that mannosyl glucosylceramide (MacCer), a species of glycosphingolipid (GSL), promotes synaptic bouton formation at the Drosophilaneuromuscular junction (NMJ). Pharmacological and genetic analysis shows that the NMJ growth-promoting effect of MacCer depends on normal lipid rafts, which are known to be composed of sphingolipids, sterols and select proteins. MacCer positively regulates the synaptic level of Wnt1/Wingless (Wg) and facilitates presynaptic Wg signaling, whose activity is raft-dependent. Furthermore, a functional GSL-binding motif in Wg exhibiting a high affinity for MacCer is required for normal NMJ growth. These findings reveal a novel mechanism whereby the GSL MacCer promotes synaptic bouton formation via Wg signaling.
|
论文编号: |
DOI:10.7554/eLife.38183 |
论文题目: |
The Glycosphingolipid MacCer Promotes Synaptic Bouton Formation in Drosophila by Interacting with Wnt |
英文论文题目: |
The Glycosphingolipid MacCer Promotes Synaptic Bouton Formation in Drosophila by Interacting with Wnt |
第一作者: |
Yan Huang, Sheng Huang, Coralie Di Scala, Qifu Wang, Hans H Wandall, Jacques Fantini, Yong Q Zhang |
英文第一作者: |
Yan Huang, Sheng Huang, Coralie Di Scala, Qifu Wang, Hans H Wandall, Jacques Fantini, Yong Q Zhang |
联系作者: |
|
英文联系作者: |
|
外单位作者单位: |
|
英文外单位作者单位: |
|
发表年度: |
2018-10-29 |
卷: |
|
期: |
|
页码: |
|
摘要: |
Lipids are structural components of cellular membranes and signaling molecules that are widely involved in development and diseases, but the underlying molecular mechanisms are poorly understood, partly because of the vast variety of lipid species and complexity of synthetic and turnover pathways. From a genetic screen, we identify that mannosyl glucosylceramide (MacCer), a species of glycosphingolipid (GSL), promotes synaptic bouton formation at the Drosophilaneuromuscular junction (NMJ). Pharmacological and genetic analysis shows that the NMJ growth-promoting effect of MacCer depends on normal lipid rafts, which are known to be composed of sphingolipids, sterols and select proteins. MacCer positively regulates the synaptic level of Wnt1/Wingless (Wg) and facilitates presynaptic Wg signaling, whose activity is raft-dependent. Furthermore, a functional GSL-binding motif in Wg exhibiting a high affinity for MacCer is required for normal NMJ growth. These findings reveal a novel mechanism whereby the GSL MacCer promotes synaptic bouton formation via Wg signaling. |
英文摘要: |
Lipids are structural components of cellular membranes and signaling molecules that are widely involved in development and diseases, but the underlying molecular mechanisms are poorly understood, partly because of the vast variety of lipid species and complexity of synthetic and turnover pathways. From a genetic screen, we identify that mannosyl glucosylceramide (MacCer), a species of glycosphingolipid (GSL), promotes synaptic bouton formation at the Drosophilaneuromuscular junction (NMJ). Pharmacological and genetic analysis shows that the NMJ growth-promoting effect of MacCer depends on normal lipid rafts, which are known to be composed of sphingolipids, sterols and select proteins. MacCer positively regulates the synaptic level of Wnt1/Wingless (Wg) and facilitates presynaptic Wg signaling, whose activity is raft-dependent. Furthermore, a functional GSL-binding motif in Wg exhibiting a high affinity for MacCer is required for normal NMJ growth. These findings reveal a novel mechanism whereby the GSL MacCer promotes synaptic bouton formation via Wg signaling. |
刊物名称: |
eLIFE |
英文刊物名称: |
eLIFE |
论文全文: |
|
英文论文全文: |
|
全文链接: |
|
其它备注: |
Yan Huang, Sheng Huang, Coralie Di Scala, Qifu Wang, Hans H Wandall, Jacques Fantini, Yong Q Zhang. The Glycosphingolipid MacCer Promotes Synaptic Bouton Formation in Drosophila by Interacting with Wnt. eLIFE. DOI:10.7554/eLife.38183 |
英文其它备注: |
|
学科: |
|
英文学科: |
|
影响因子: |
|
第一作者所在部门: |
|
英文第一作者所在部门: |
|
论文出处: |
|
英文论文出处: |
|
论文类别: |
|
英文论文类别: |
|
参与作者: |
|
英文参与作者: |
|
|