|
BICELLULAR POLLEN 1 is a Modulator of DNA Replication and Pollen Development in Arabidopsis
Yanping Long, Dongjiang Xie, Yanyan Zhao, Dongqiao Shi, Weicai Yang
New Phytologist
Abstract
During male gametogenesis in Arabidopsis, the haploid microspore undergoes an asymmetric division to produce a vegetative and a generative cell, the latter of which continues to divide symmetrically to form two sperms. This simple system couples cell cycle with cell fate specification. Here we addressed the role of DNA replication in male gametogenesis using a mutant bicellular pollen 1 (bice1), which produces bicellular, rather than tricellular, pollen grains as in the wild‐type plant at anthesis. The mutation prolonged DNA synthesis of the generative cell, which resulted in c. 40% of pollen grains arrested at the two‐nucleate stage. The extended S phase did not impact the cell fate of the generative cell as shown by cell‐specific markers. BICE1 encodes a plant homolog of human D123 protein that is required for G1 progression, but the underlying mechanism is unknown. Here we showed that BICE1 interacts with MCM4 and MCM7 of the pre‐replication complex. Consistently, double mutations in BICE1 and MCM4, or MCM7, also led to bicellular pollen and condensed chromosomes. These suggest that BICE1 plays a role in modulating DNA replication via interaction with MCM4 and MCM7.
|
论文编号: |
DOI:10.1111/nph.15610 |
论文题目: |
BICELLULAR POLLEN 1 is a Modulator of DNA Replication and Pollen Development in Arabidopsis |
英文论文题目: |
BICELLULAR POLLEN 1 is a Modulator of DNA Replication and Pollen Development in Arabidopsis |
第一作者: |
Yanping Long, Dongjiang Xie, Yanyan Zhao, Dongqiao Shi, Weicai Yang |
英文第一作者: |
Yanping Long, Dongjiang Xie, Yanyan Zhao, Dongqiao Shi, Weicai Yang |
联系作者: |
|
英文联系作者: |
|
外单位作者单位: |
|
英文外单位作者单位: |
|
发表年度: |
2019-01-24 |
卷: |
|
期: |
|
页码: |
|
摘要: |
During male gametogenesis in Arabidopsis, the haploid microspore undergoes an asymmetric division to produce a vegetative and a generative cell, the latter of which continues to divide symmetrically to form two sperms. This simple system couples cell cycle with cell fate specification. Here we addressed the role of DNA replication in male gametogenesis using a mutant bicellular pollen 1 (bice1), which produces bicellular, rather than tricellular, pollen grains as in the wild‐type plant at anthesis. The mutation prolonged DNA synthesis of the generative cell, which resulted in c. 40% of pollen grains arrested at the two‐nucleate stage. The extended S phase did not impact the cell fate of the generative cell as shown by cell‐specific markers. BICE1 encodes a plant homolog of human D123 protein that is required for G1 progression, but the underlying mechanism is unknown. Here we showed that BICE1 interacts with MCM4 and MCM7 of the pre‐replication complex. Consistently, double mutations in BICE1 and MCM4, or MCM7, also led to bicellular pollen and condensed chromosomes. These suggest that BICE1 plays a role in modulating DNA replication via interaction with MCM4 and MCM7. |
英文摘要: |
During male gametogenesis in Arabidopsis, the haploid microspore undergoes an asymmetric division to produce a vegetative and a generative cell, the latter of which continues to divide symmetrically to form two sperms. This simple system couples cell cycle with cell fate specification. Here we addressed the role of DNA replication in male gametogenesis using a mutant bicellular pollen 1 (bice1), which produces bicellular, rather than tricellular, pollen grains as in the wild‐type plant at anthesis. The mutation prolonged DNA synthesis of the generative cell, which resulted in c. 40% of pollen grains arrested at the two‐nucleate stage. The extended S phase did not impact the cell fate of the generative cell as shown by cell‐specific markers. BICE1 encodes a plant homolog of human D123 protein that is required for G1 progression, but the underlying mechanism is unknown. Here we showed that BICE1 interacts with MCM4 and MCM7 of the pre‐replication complex. Consistently, double mutations in BICE1 and MCM4, or MCM7, also led to bicellular pollen and condensed chromosomes. These suggest that BICE1 plays a role in modulating DNA replication via interaction with MCM4 and MCM7. |
刊物名称: |
New Phytologist |
英文刊物名称: |
New Phytologist |
论文全文: |
|
英文论文全文: |
|
全文链接: |
|
其它备注: |
Yanping Long, Dongjiang Xie, Yanyan Zhao, Dongqiao Shi, Weicai Yang. BICELLULAR POLLEN 1 is a Modulator of DNA Replication and Pollen Development in Arabidopsis. New Phytologist. DOI:10.1111/nph.15610 |
英文其它备注: |
|
学科: |
|
英文学科: |
|
影响因子: |
|
第一作者所在部门: |
|
英文第一作者所在部门: |
|
论文出处: |
|
英文论文出处: |
|
论文类别: |
|
英文论文类别: |
|
参与作者: |
|
英文参与作者: |
|
|