|
Generation of Herbicide Tolerance Traits and a New Selectable Marker in Wheat Using Base-editing
Rui Zhang, Jinxing Liu, Zhuangzhuang Chai, Sha Chen, Yang Bai, Yuan Zong, Kunling Chen, Jiayang Li, Linjian Jiang and Caixia Gao
Nature Plants
Abstract
Developing herbicide tolerant (HT) varieties by genome editing holds great promise for addressing the worsening weed problems in wheat cultivation. Here we generated transgene-free wheat germplasms harboring HT mutations that confer tolerance to sulfonylurea-, imidazolinone- and aryloxyphenoxy propionate-type herbicides by base editing the acetolactate synthase (ALS) and acetyl-coenzyme A carboxylase (ACCase) genes. These stackable HT traits provide a potentially powerful tool for weed management. In addition, we found that base editing at the wheat ALS P174 codon (TaALS-P174) endowed wheat with sufficient resistance to nicosulfuron herbicide in MS growth medium to allow selection. When the TaALS-P174 editor was coupled with editors for other targets of interest, co-editing occurred in the nicosulfuron-resistant plants, and selection for resistance in growth medium enriched the frequency of coupled targets by several-fold. This selectable co-editing system has the potential to greatly bolster adoption of base editing for crop improvement applications.
|
论文编号: |
DOI:10.1038/s41477-019-0405-0 |
论文题目: |
Generation of Herbicide Tolerance Traits and a New Selectable Marker in Wheat Using Base-editing |
英文论文题目: |
Generation of Herbicide Tolerance Traits and a New Selectable Marker in Wheat Using Base-editing |
第一作者: |
Rui Zhang, Jinxing Liu, Zhuangzhuang Chai, Sha Chen, Yang Bai, Yuan Zong, Kunling Chen, Jiayang Li, Linjian Jiang and Caixia Gao |
英文第一作者: |
Rui Zhang, Jinxing Liu, Zhuangzhuang Chai, Sha Chen, Yang Bai, Yuan Zong, Kunling Chen, Jiayang Li, Linjian Jiang and Caixia Gao |
联系作者: |
|
英文联系作者: |
|
外单位作者单位: |
|
英文外单位作者单位: |
|
发表年度: |
2019-04-16 |
卷: |
|
期: |
|
页码: |
|
摘要: |
Developing herbicide tolerant (HT) varieties by genome editing holds great promise for addressing the worsening weed problems in wheat cultivation. Here we generated transgene-free wheat germplasms harboring HT mutations that confer tolerance to sulfonylurea-, imidazolinone- and aryloxyphenoxy propionate-type herbicides by base editing the acetolactate synthase (ALS) and acetyl-coenzyme A carboxylase (ACCase) genes. These stackable HT traits provide a potentially powerful tool for weed management. In addition, we found that base editing at the wheat ALS P174 codon (TaALS-P174) endowed wheat with sufficient resistance to nicosulfuron herbicide in MS growth medium to allow selection. When the TaALS-P174 editor was coupled with editors for other targets of interest, co-editing occurred in the nicosulfuron-resistant plants, and selection for resistance in growth medium enriched the frequency of coupled targets by several-fold. This selectable co-editing system has the potential to greatly bolster adoption of base editing for crop improvement applications. |
英文摘要: |
Developing herbicide tolerant (HT) varieties by genome editing holds great promise for addressing the worsening weed problems in wheat cultivation. Here we generated transgene-free wheat germplasms harboring HT mutations that confer tolerance to sulfonylurea-, imidazolinone- and aryloxyphenoxy propionate-type herbicides by base editing the acetolactate synthase (ALS) and acetyl-coenzyme A carboxylase (ACCase) genes. These stackable HT traits provide a potentially powerful tool for weed management. In addition, we found that base editing at the wheat ALS P174 codon (TaALS-P174) endowed wheat with sufficient resistance to nicosulfuron herbicide in MS growth medium to allow selection. When the TaALS-P174 editor was coupled with editors for other targets of interest, co-editing occurred in the nicosulfuron-resistant plants, and selection for resistance in growth medium enriched the frequency of coupled targets by several-fold. This selectable co-editing system has the potential to greatly bolster adoption of base editing for crop improvement applications. |
刊物名称: |
Nature Plants |
英文刊物名称: |
Nature Plants |
论文全文: |
|
英文论文全文: |
|
全文链接: |
|
其它备注: |
Rui Zhang, Jinxing Liu, Zhuangzhuang Chai, Sha Chen, Yang Bai, Yuan Zong, Kunling Chen, Jiayang Li, Linjian Jiang and Caixia Gao. Generation of Herbicide Tolerance Traits and a New Selectable Marker in Wheat Using Base-editing. Nature Plants. DOI:10.1038/s41477-019-0405-0 |
英文其它备注: |
|
学科: |
|
英文学科: |
|
影响因子: |
|
第一作者所在部门: |
|
英文第一作者所在部门: |
|
论文出处: |
|
英文论文出处: |
|
论文类别: |
|
英文论文类别: |
|
参与作者: |
|
英文参与作者: |
|
|