|
Regulation of Rice Tillering by RNA-directed DNA Methylation at Miniature Inverted-repeat Transposable Elements
Le Xu, Kun Yuan, Meng Yuan, Xiangbing Meng, Min Chen, Jianguo Wu, Jiayang Li, Yijun Qi
Molecular Plant
Abstract
Tillering is a major determinant of rice plant architecture and grain yield. Here we report that depletion of rice OsNRPD1a and OsNRPD1b, two orthologues of the largest subunit of RNA polymerase IV, leads to a high-tillering phenotype, in addition to dwarfism and smaller panicles. OsNRPD1a and OsNRPD1b are required for the production of 24-nt siRNAs that direct DNA methylation at transposable elements (TEs) including Miniature Inverted-repeat TEs (MITEs). Interestingly, many genes are regulated either positively or negatively by TE methylation. Among them, OsMIR156d and OsMIR156j, which promote rice tillering, are repressed by CHH methylation at two MITEs in the promoters. By contrast, D14, which suppresses rice tillering, is activated by CHH methylation at a MITE in its downstream. Our findings reveal control of rice tillering by RdDM at MITEs and provide potential targets for agronomic trait enhancement by epigenome editing.
|
论文编号: |
DOI:10.1016/j.molp.2020.02.009 |
论文题目: |
Regulation of Rice Tillering by RNA-directed DNA Methylation at Miniature Inverted-repeat Transposable Elements |
英文论文题目: |
Regulation of Rice Tillering by RNA-directed DNA Methylation at Miniature Inverted-repeat Transposable Elements |
第一作者: |
Le Xu, Kun Yuan, Meng Yuan, Xiangbing Meng, Min Chen, Jianguo Wu, Jiayang Li, Yijun Qi |
英文第一作者: |
Le Xu, Kun Yuan, Meng Yuan, Xiangbing Meng, Min Chen, Jianguo Wu, Jiayang Li, Yijun Qi |
联系作者: |
|
英文联系作者: |
|
外单位作者单位: |
|
英文外单位作者单位: |
|
发表年度: |
2020-02-28 |
卷: |
|
期: |
|
页码: |
|
摘要: |
Tillering is a major determinant of rice plant architecture and grain yield. Here we report that depletion of rice OsNRPD1a and OsNRPD1b, two orthologues of the largest subunit of RNA polymerase IV, leads to a high-tillering phenotype, in addition to dwarfism and smaller panicles. OsNRPD1a and OsNRPD1b are required for the production of 24-nt siRNAs that direct DNA methylation at transposable elements (TEs) including Miniature Inverted-repeat TEs (MITEs). Interestingly, many genes are regulated either positively or negatively by TE methylation. Among them, OsMIR156d and OsMIR156j, which promote rice tillering, are repressed by CHH methylation at two MITEs in the promoters. By contrast, D14, which suppresses rice tillering, is activated by CHH methylation at a MITE in its downstream. Our findings reveal control of rice tillering by RdDM at MITEs and provide potential targets for agronomic trait enhancement by epigenome editing. |
英文摘要: |
Tillering is a major determinant of rice plant architecture and grain yield. Here we report that depletion of rice OsNRPD1a and OsNRPD1b, two orthologues of the largest subunit of RNA polymerase IV, leads to a high-tillering phenotype, in addition to dwarfism and smaller panicles. OsNRPD1a and OsNRPD1b are required for the production of 24-nt siRNAs that direct DNA methylation at transposable elements (TEs) including Miniature Inverted-repeat TEs (MITEs). Interestingly, many genes are regulated either positively or negatively by TE methylation. Among them, OsMIR156d and OsMIR156j, which promote rice tillering, are repressed by CHH methylation at two MITEs in the promoters. By contrast, D14, which suppresses rice tillering, is activated by CHH methylation at a MITE in its downstream. Our findings reveal control of rice tillering by RdDM at MITEs and provide potential targets for agronomic trait enhancement by epigenome editing. |
刊物名称: |
Molecular Plant |
英文刊物名称: |
Molecular Plant |
论文全文: |
|
英文论文全文: |
|
全文链接: |
|
其它备注: |
Le Xu, Kun Yuan, Meng Yuan, Xiangbing Meng, Min Chen, Jianguo Wu, Jiayang Li, Yijun Qi. Regulation of Rice Tillering by RNA-directed DNA Methylation at Miniature Inverted-repeat Transposable Elements. Molecular Plant. DOI:10.1016/j.molp.2020.02.009 |
英文其它备注: |
|
学科: |
|
英文学科: |
|
影响因子: |
|
第一作者所在部门: |
|
英文第一作者所在部门: |
|
论文出处: |
|
英文论文出处: |
|
论文类别: |
|
英文论文类别: |
|
参与作者: |
|
英文参与作者: |
|
|